AB174268

Anti-TRIML1 antibody [EPR12520]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal TRIML1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RNF209, TRIML1, Probable E3 ubiquitin-protein ligase TRIML1, RING finger protein 209, RING-type E3 ubiquitin transferase TRIML1, Tripartite motif family-like protein 1

1 Images

Supplier Data

Western blot - Anti-TRIML1 antibody [EPR12520] (AB174268)

All lanes:

Western blot - Anti-TRIML1 antibody [EPR12520] (ab174268) at 1/1000 dilution

Lane 1:

293T lysates at 10 µg

Lane 2:

Human placenta lysates at 10 µg

Predicted band size: 53 kDa

false

Carrier free

Anti-TRIML1 antibody [EPR12520] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12520

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIML1 also known as Tripartite Motif Containing Like 1 is a protein involved in E3 ubiquitin ligase activity. The protein promotes ubiquitination by attaching ubiquitin molecules to substrate proteins tagging them for various cellular fates. TRIML1 weighs approximately 74 kDa and it is expressed in various tissues including immune cells and specific brain regions. The protein’s modular structure consists of a RING finger domain B-boxes and a coiled-coil region which aids in its regulatory functions.

Biological function summary

The protein plays a role in immune response regulation and maintaining protein homeostasis. TRIML1 does not exist as part of a large complex but interacts directly with specific substrates and signaling molecules. The RING finger domain facilitates its binding to E2 ubiquitin-conjugating enzyme enhancing its function in protein degradation and signal transduction. Furthermore TRIML1 modulates inflammation by its action on pro-inflammatory signaling pathways.

Pathways

TRIML1 is engaged mainly in the ubiquitin-proteasome system and the inflammatory signaling pathways. In the context of the ubiquitin-proteasome system TRIML1 influences protein turnover and cellular responses to stress. Additionally it participates in the regulation of NF-kB signaling which is essential for inflammatory responses. Its interactions with proteins such as UBE2D serve as critical connections in these pathways assisting in both protein ubiquitination and inflammatory regulation.

TRIML1 shows connections particularly to neurodegenerative diseases and certain immune disorders. Alterations in TRIML1 expression or function can contribute to proteostasis imbalances implicated in disorders such as Alzheimer's disease. The protein's influence on inflammatory pathways suggests a potential role in autoimmune conditions. In these contexts proteins like NF-kB become highly relevant as both TRIML1 and NF-kB coordinate immune signaling which can exacerbate or mitigate disease development.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Probable E3 ubiquitin-protein ligase which plays an important role in blastocyst development.

See full target information TRIML1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 10:644 PubMed31501420

2019

CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Liu,Xiao Zhang,Jiafei Lin,Yuxin Chen,Yongxia Qiao,Susu Guo,Yueyue Yang,Guoqing Zhu,Qiuhui Pan,Jiayi Wang,Fenyong Sun

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIML1 antibody [EPR12520]

Western blot - Anti-TRIML1 antibody [EPR12520] (AB174268)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise