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AB249779

Anti-TRIML1 antibody [EPR12520] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TRIML1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RNF209, TRIML1, Probable E3 ubiquitin-protein ligase TRIML1, RING finger protein 209, RING-type E3 ubiquitin transferase TRIML1, Tripartite motif family-like protein 1

1 Images
Western blot - Anti-TRIML1 antibody [EPR12520] - BSA and Azide free (AB249779)
  • WB

Supplier Data

Western blot - Anti-TRIML1 antibody [EPR12520] - BSA and Azide free (AB249779)

This data was developed using ab174268, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TRIML1 antibody [EPR12520] (<a href='/en-us/products/primary-antibodies/triml1-antibody-epr12520-ab174268'>ab174268</a>) at 1/1000 dilution

Lane 1:

293T lysates at 10 µg

Lane 2:

Human placenta lysates at 10 µg

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12520

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249779 is the carrier-free version of ab174268.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIML1 also known as Tripartite Motif Containing Like 1 is a protein involved in E3 ubiquitin ligase activity. The protein promotes ubiquitination by attaching ubiquitin molecules to substrate proteins tagging them for various cellular fates. TRIML1 weighs approximately 74 kDa and it is expressed in various tissues including immune cells and specific brain regions. The protein’s modular structure consists of a RING finger domain B-boxes and a coiled-coil region which aids in its regulatory functions.
Biological function summary

The protein plays a role in immune response regulation and maintaining protein homeostasis. TRIML1 does not exist as part of a large complex but interacts directly with specific substrates and signaling molecules. The RING finger domain facilitates its binding to E2 ubiquitin-conjugating enzyme enhancing its function in protein degradation and signal transduction. Furthermore TRIML1 modulates inflammation by its action on pro-inflammatory signaling pathways.

Pathways

TRIML1 is engaged mainly in the ubiquitin-proteasome system and the inflammatory signaling pathways. In the context of the ubiquitin-proteasome system TRIML1 influences protein turnover and cellular responses to stress. Additionally it participates in the regulation of NF-kB signaling which is essential for inflammatory responses. Its interactions with proteins such as UBE2D serve as critical connections in these pathways assisting in both protein ubiquitination and inflammatory regulation.

TRIML1 shows connections particularly to neurodegenerative diseases and certain immune disorders. Alterations in TRIML1 expression or function can contribute to proteostasis imbalances implicated in disorders such as Alzheimer's disease. The protein's influence on inflammatory pathways suggests a potential role in autoimmune conditions. In these contexts proteins like NF-kB become highly relevant as both TRIML1 and NF-kB coordinate immune signaling which can exacerbate or mitigate disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable E3 ubiquitin-protein ligase which plays an important role in blastocyst development.
See full target information TRIML1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com