Rabbit Polyclonal TRIOBP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRIO and F-actin-binding protein aa 1800-2100.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes 10mM Citrate buffer Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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Isoform 1Regulates actin cytoskeletal organization, cell spreading and cell contraction by directly binding and stabilizing filamentous F-actin and prevents its depolymerization (PubMed:18194665, PubMed:28438837). May also serve as a linker protein to recruit proteins required for F-actin formation and turnover (PubMed:18194665). Essential for correct mitotic progression (PubMed:22820163, PubMed:24692559).Isoform 5Plays a pivotal role in the formation of stereocilia rootlets.Isoform 4Plays a pivotal role in the formation of stereocilia rootlets.
KIAA1662, TARA, HRIHFB2122, TRIOBP, KIAA1662, TARA, HRIHFB2122, TRIO and F-actin-binding protein, Protein Tara, TRF1-associated protein of 68 kDa, Trio-associated repeat on actin
Rabbit Polyclonal TRIOBP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRIO and F-actin-binding protein aa 1800-2100.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The TRIOBP protein also known as Trio-Binding Protein has a molecular mass of approximately 75 kDa though it may vary depending on the isoform. It localizes in various tissues with strong expression in the brain suggesting a role in neural processes. TRIOBP interacts with the actin cytoskeleton mainly influencing cell shape and movement. It is known for binding to Trio a guanine nucleotide exchange factor involved in actin rearrangement which highlights its importance in maintaining structural integrity at the cellular level.
This protein influences cellular dynamics by maintaining cytoskeletal architecture. TRIOBP can exist in several isoforms implicated in different cellular processes. It interacts with signaling complexes typically playing roles in neuronal development and synaptic functioning. In the context of cellular networks TRIOBP's involvement in communication pathways between cells supports its significance in maintaining efficient cellular communication.
TRIOBP integrates into regulatory systems impacting neuronal signaling and actin cytoskeleton reorganization. The protein operates within pathways including the Rho GTPase signaling where it connects with proteins like Filamin A to mediate cytoskeletal alterations. It is also associated with pathways influencing neuronal growth working alongside DLG family proteins pivotal in synaptic signaling and plasticity.
Mutations or modifications in TRIOBP link to certain health conditions. It associates notably with schizophrenia where altered expression impacts neural connectivity. The protein also bears relevance to hearing loss interacting closely with DFNB28 gene products that affect inner ear hair cell function. Both conditions illustrate the protein's influence on nervous system integrity and sensory processing.
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10% SDS PAGE
All lanes: Western blot - Anti-TRIOBP antibody - C-terminal (ab151320) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 30 µg
Lane 2: HepG2 whole cell lysate at 30 µg
Predicted band size: 261 kDa
10% SDS PAGE
All lanes: Western blot - Anti-TRIOBP antibody - C-terminal (ab151320) at 1/1000 dilution
All lanes: NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 261 kDa
Immunohistochemical analysis of paraffin-embedded Human MDA-MB-157 xenograft tissue labeling TRIOBP with ab151320 at 1/100 dilution.
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling TRIOBP using ab151320 at 1/200 dilution. Right image shows cells co-stained with Hoechst 33342.
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