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Rabbit Polyclonal TRIOBP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRIO and F-actin-binding protein aa 1800-2100.

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Images

Western blot - Anti-TRIOBP antibody - C-terminal (AB151320), expandable thumbnail
  • Western blot - Anti-TRIOBP antibody - C-terminal (AB151320), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIOBP antibody - C-terminal (AB151320), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TRIOBP antibody - C-terminal (AB151320), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human TRIO and F-actin-binding protein aa 1800-2100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H2D6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

10mM Citrate buffer

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Isoform 1Regulates actin cytoskeletal organization, cell spreading and cell contraction by directly binding and stabilizing filamentous F-actin and prevents its depolymerization (PubMed:18194665, PubMed:28438837). May also serve as a linker protein to recruit proteins required for F-actin formation and turnover (PubMed:18194665). Essential for correct mitotic progression (PubMed:22820163, PubMed:24692559).Isoform 5Plays a pivotal role in the formation of stereocilia rootlets.Isoform 4Plays a pivotal role in the formation of stereocilia rootlets.

Alternative names

Recommended products

Rabbit Polyclonal TRIOBP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRIO and F-actin-binding protein aa 1800-2100.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human TRIO and F-actin-binding protein aa 1800-2100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H2D6
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The TRIOBP protein also known as Trio-Binding Protein has a molecular mass of approximately 75 kDa though it may vary depending on the isoform. It localizes in various tissues with strong expression in the brain suggesting a role in neural processes. TRIOBP interacts with the actin cytoskeleton mainly influencing cell shape and movement. It is known for binding to Trio a guanine nucleotide exchange factor involved in actin rearrangement which highlights its importance in maintaining structural integrity at the cellular level.

Biological function summary

This protein influences cellular dynamics by maintaining cytoskeletal architecture. TRIOBP can exist in several isoforms implicated in different cellular processes. It interacts with signaling complexes typically playing roles in neuronal development and synaptic functioning. In the context of cellular networks TRIOBP's involvement in communication pathways between cells supports its significance in maintaining efficient cellular communication.

Pathways

TRIOBP integrates into regulatory systems impacting neuronal signaling and actin cytoskeleton reorganization. The protein operates within pathways including the Rho GTPase signaling where it connects with proteins like Filamin A to mediate cytoskeletal alterations. It is also associated with pathways influencing neuronal growth working alongside DLG family proteins pivotal in synaptic signaling and plasticity.

Associated diseases and disorders

Mutations or modifications in TRIOBP link to certain health conditions. It associates notably with schizophrenia where altered expression impacts neural connectivity. The protein also bears relevance to hearing loss interacting closely with DFNB28 gene products that affect inner ear hair cell function. Both conditions illustrate the protein's influence on nervous system integrity and sensory processing.

Product promise

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4 product images

  • Western blot - Anti-TRIOBP antibody - C-terminal (ab151320), expandable thumbnail

    Western blot - Anti-TRIOBP antibody - C-terminal (ab151320)

    10% SDS PAGE

    All lanes: Western blot - Anti-TRIOBP antibody - C-terminal (ab151320) at 1/1000 dilution

    Lane 1: HeLa whole cell lysate at 30 µg

    Lane 2: HepG2 whole cell lysate at 30 µg

    Predicted band size: 261 kDa

  • Western blot - Anti-TRIOBP antibody - C-terminal (ab151320), expandable thumbnail

    Western blot - Anti-TRIOBP antibody - C-terminal (ab151320)

    10% SDS PAGE

    All lanes: Western blot - Anti-TRIOBP antibody - C-terminal (ab151320) at 1/1000 dilution

    All lanes: NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 261 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIOBP antibody - C-terminal (ab151320), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIOBP antibody - C-terminal (ab151320)

    Immunohistochemical analysis of paraffin-embedded Human MDA-MB-157 xenograft tissue labeling TRIOBP with ab151320 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRIOBP antibody - C-terminal (ab151320), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TRIOBP antibody - C-terminal (ab151320)

    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling TRIOBP using ab151320 at 1/200 dilution. Right image shows cells co-stained with Hoechst 33342.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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