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AB151320

Anti-TRIOBP antibody - C-terminal

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(2 Publications)

Rabbit Polyclonal TRIOBP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIOBP aa 1800-2100.

View Alternative Names

KIAA1662, TARA, HRIHFB2122, TRIOBP, TRIO and F-actin-binding protein, Protein Tara, TRF1-associated protein of 68 kDa, Trio-associated repeat on actin

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TRIOBP antibody - C-terminal (AB151320)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRIOBP antibody - C-terminal (AB151320)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling TRIOBP using ab151320 at 1/200 dilution. Right image shows cells co-stained with Hoechst 33342.

Western blot - Anti-TRIOBP antibody - C-terminal (AB151320)
  • WB

Supplier Data

Western blot - Anti-TRIOBP antibody - C-terminal (AB151320)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-TRIOBP antibody - C-terminal (ab151320) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 261 kDa

false

Western blot - Anti-TRIOBP antibody - C-terminal (AB151320)
  • WB

Supplier Data

Western blot - Anti-TRIOBP antibody - C-terminal (AB151320)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-TRIOBP antibody - C-terminal (ab151320) at 1/5000 dilution

All lanes:

Rat2 cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 261 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TRIOBP aa 1800-2100. The exact immunogen used to generate this antibody is proprietary information.

Q9H2D6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>10mM Citrate buffer</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TRIOBP protein also known as Trio-Binding Protein has a molecular mass of approximately 75 kDa though it may vary depending on the isoform. It localizes in various tissues with strong expression in the brain suggesting a role in neural processes. TRIOBP interacts with the actin cytoskeleton mainly influencing cell shape and movement. It is known for binding to Trio a guanine nucleotide exchange factor involved in actin rearrangement which highlights its importance in maintaining structural integrity at the cellular level.
Biological function summary

This protein influences cellular dynamics by maintaining cytoskeletal architecture. TRIOBP can exist in several isoforms implicated in different cellular processes. It interacts with signaling complexes typically playing roles in neuronal development and synaptic functioning. In the context of cellular networks TRIOBP's involvement in communication pathways between cells supports its significance in maintaining efficient cellular communication.

Pathways

TRIOBP integrates into regulatory systems impacting neuronal signaling and actin cytoskeleton reorganization. The protein operates within pathways including the Rho GTPase signaling where it connects with proteins like Filamin A to mediate cytoskeletal alterations. It is also associated with pathways influencing neuronal growth working alongside DLG family proteins pivotal in synaptic signaling and plasticity.

Mutations or modifications in TRIOBP link to certain health conditions. It associates notably with schizophrenia where altered expression impacts neural connectivity. The protein also bears relevance to hearing loss interacting closely with DFNB28 gene products that affect inner ear hair cell function. Both conditions illustrate the protein's influence on nervous system integrity and sensory processing.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Regulates actin cytoskeletal organization, cell spreading and cell contraction by directly binding and stabilizing filamentous F-actin and prevents its depolymerization (PubMed : 18194665, PubMed : 28438837). May also serve as a linker protein to recruit proteins required for F-actin formation and turnover (PubMed : 18194665). Essential for correct mitotic progression (PubMed : 22820163, PubMed : 24692559).. Isoform 5. Plays a pivotal role in the formation of stereocilia rootlets.. Isoform 4. Plays a pivotal role in the formation of stereocilia rootlets.
See full target information TRIOBP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 81:13 PubMed38157020

2024

TRIOBP modulates β-catenin signaling by regulation of miR-29b in idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Wang,Wenyu Zhao,Cong Xia,Shuaichen Ma,Zhongzheng Li,Ningdan Wang,Linke Ding,Yaxuan Wang,Lianhui Cheng,Huibing Liu,Juntang Yang,Yajun Li,Ivan Rosas,Guoying Yu

Journal of cell science 131: PubMed29507111

2018

Localization and functional consequences of a direct interaction between TRIOBP-1 and hERG proteins in the heart.

Applications

Unspecified application

Species

Unspecified reactive species

David K Jones,Ashley C Johnson,Elon C Roti Roti,Fang Liu,Rebecca Uelmen,Rebecca A Ayers,Istvan Baczko,David J Tester,Michael J Ackerman,Matthew C Trudeau,Gail A Robertson
View all publications
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