AB196618

Anti-Triosephosphate isomerase antibody

Name: Anti-Triosephosphate isomerase antibody (ab196618) | Abcam
Brand: Abcam Limited
SKU: AB196618

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(3 Publications)

Rabbit Polyclonal Triosephosphate isomerase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TPI1.

View Alternative Names

TPI, TPI1, Triosephosphate isomerase, TIM, Methylglyoxal synthase, Triose-phosphate isomerase

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Triosephosphate isomerase antibody (AB196618)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Triosephosphate isomerase antibody (AB196618)

Immunofluorescence analysis of HeLa cells, labeling Triosephosphate isomerase using ab196618 at a 1/50 dilution. Blue : DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triosephosphate isomerase antibody (AB196618)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triosephosphate isomerase antibody (AB196618)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Triosephosphate isomerase using ab196618 at a 1/200 dilution

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triosephosphate isomerase antibody (AB196618)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Triosephosphate isomerase using ab196618 at a 1/200 dilution.

Supplier Data

Western blot - Anti-Triosephosphate isomerase antibody (AB196618)

All lanes:

Western blot - Anti-Triosephosphate isomerase antibody (ab196618) at 1/500 dilution

Lane 1:

22RV-1 cell lysate

Lane 2:

BT474 cell lysate

Lane 3:

HeLa cell lysate

Lane 4:

Jurkat cell lysate

Lane 5:

U251 cell lysate

Lane 6:

HepG2 cell lysate

Lane 7:

mouse kidney lysate

Lane 8:

mouse brain lysate

Lane 9:

mouse heart lysate

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TPI1.

P60174

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Triosephosphate isomerase also known as TPI is an enzyme that plays an important role in glycolysis and gluconeogenesis by catalyzing the reversible isomerization of dihydroxyacetone phosphate (DHAP) to glyceraldehyde 3-phosphate (G3P). It has a molecular mass of approximately 27 kDa. This enzyme is widely expressed in many tissues with high presence in red blood cells muscle and brain tissues due to their high energy demands. As a dimer enzyme it exhibits significant catalytic efficiency that supports cellular energy management.

Biological function summary

The enzyme facilitates the efficient production of ATP by maintaining balance between DHAP and G3P vital for energy-metabolizing cells. Triosephosphate isomerase is not part of a larger complex itself but it significantly interacts with other enzymes in the glycolytic pathway. This interaction supports cellular respiration by ensuring a steady supply of intermediates needed for downstream processes.

Pathways

Triosephosphate isomerase is central to glycolysis and gluconeogenesis pathways. In glycolysis it works in tandem with other enzymes such as hexokinase and phosphofructokinase to breakdown glucose into pyruvate yielding ATP and NADH. It provides a critical link between glycolysis and energy production processes impacting the cellular metabolism and biochemical energy cycles. The enzyme also contributes to gluconeogenesis where it helps synthesize glucose from non-carbohydrate sources ensuring energy supply during fasting.

Mutations or deficiencies in triosephosphate isomerase can lead to conditions such as triosephosphate isomerase deficiency a rare autosomal recessive disorder characterized by progressive neurological dysfunction hemolytic anemia and muscle weakness. This deficiency arises from dysfunctional or unstable isomerase activity leading to an accumulation of DHAP. Additionally research has shown links between this enzyme and Alzheimer's disease where altered glucose metabolism is a common feature. Abnormal triosephosphate isomerase activity may interact with amyloid precursor protein processing in the disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.. It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.

See full target information TPI1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39456698

2024

Role of IL3RA in a Family with Lumbar Spinal Stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Ming Liu,Chi-Fan Yang,Weng-Siong H'ng,Hui-Ping Chuang,Eunice Han Xian Khor,Pei-Chun Tsai,Vivia Khosasih,Liang-Suei Lu,Erh-Chan Yeh,Wan-Jia Lin,Feng-Jen Hsieh,Chien-Hsiun Chen,Shiuh-Lin Hwang,Jer-Yuarn Wu

Journal of cellular and molecular medicine 27:1664-1681 PubMed37132040

2023

Lyn-mediated glycolysis enhancement of microglia contributes to neuropathic pain through facilitating IRF5 nuclear translocation in spinal dorsal horn.

Applications

Unspecified application

Species

Unspecified reactive species

Erliang Kong,Yongchang Li,Peng Ma,Yixuan Zhang,Ruifeng Ding,Tong Hua,Mei Yang,Hongbin Yuan

Stem cells international 2022:4363632 PubMed35069747

2022

Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Qin Li,Hui-Jie Jiang,Xiu-Yun Su,Li Feng,Na-Zhi Zhan,Shan-Shan Li,Zi-Jie Chen,Bo-Han Chang,Peng-Zhen Cheng,Liu Yang,Guo-Xian Pei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Triosephosphate isomerase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Triosephosphate isomerase antibody (AB196618)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triosephosphate isomerase antibody (AB196618)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triosephosphate isomerase antibody (AB196618)

Western blot - Anti-Triosephosphate isomerase antibody (AB196618)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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