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AB28760

Anti-Triosephosphate isomerase antibody

4

(2 Reviews)

|

(13 Publications)

Goat Polyclonal Triosephosphate isomerase antibody. Suitable for WB, IHC-P, Flow Cyt and reacts with Human, Mouse, Dog samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human TPI1 aa 200 to C-terminus.

View Alternative Names

TPI, TPI1, Triosephosphate isomerase, TIM, Methylglyoxal synthase, Triose-phosphate isomerase

4 Images
Flow Cytometry - Anti-Triosephosphate isomerase antibody (AB28760)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Triosephosphate isomerase antibody (AB28760)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triosephosphate isomerase antibody (AB28760)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triosephosphate isomerase antibody (AB28760)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, staining Triosephosphate isomerase with ab28760 at 2 μg/mL. Antigen retrieval was performed by heat mediation in a Tris/EDTA buffer (pH 9).

Western blot - Anti-Triosephosphate isomerase antibody (AB28760)
  • WB

Supplier Data

Western blot - Anti-Triosephosphate isomerase antibody (AB28760)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-Triosephosphate isomerase antibody (ab28760) at 0.001 µg/mL

Lane 1:

HepG2 cell lysate at 35 µg

Lane 2:

HEK293 cell lysate at 35 µg

Lane 3:

HeLa cell lysate at 35 µg

Lane 4:

Jurkat cell lysate in RIPA buffer at 35 µg

Predicted band size: 31 kDa

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Western blot - Anti-Triosephosphate isomerase antibody (AB28760)
  • WB

Supplier Data

Western blot - Anti-Triosephosphate isomerase antibody (AB28760)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-Triosephosphate isomerase antibody (ab28760) at 0.001 µg/mL

Lane 1:

RAW264.7 cell lysate at 35 µg

Lane 2:

KNRK cell lysate at 35 µg

Lane 3:

MDCK cell lysate at 35 µg

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Dog, Human, Mouse

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human TPI1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P60174

Specificity

This antibody is expected to recognise both reported isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.01 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-5 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.01 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.01 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Triosephosphate isomerase also known as TPI is an enzyme that plays an important role in glycolysis and gluconeogenesis by catalyzing the reversible isomerization of dihydroxyacetone phosphate (DHAP) to glyceraldehyde 3-phosphate (G3P). It has a molecular mass of approximately 27 kDa. This enzyme is widely expressed in many tissues with high presence in red blood cells muscle and brain tissues due to their high energy demands. As a dimer enzyme it exhibits significant catalytic efficiency that supports cellular energy management.
Biological function summary

The enzyme facilitates the efficient production of ATP by maintaining balance between DHAP and G3P vital for energy-metabolizing cells. Triosephosphate isomerase is not part of a larger complex itself but it significantly interacts with other enzymes in the glycolytic pathway. This interaction supports cellular respiration by ensuring a steady supply of intermediates needed for downstream processes.

Pathways

Triosephosphate isomerase is central to glycolysis and gluconeogenesis pathways. In glycolysis it works in tandem with other enzymes such as hexokinase and phosphofructokinase to breakdown glucose into pyruvate yielding ATP and NADH. It provides a critical link between glycolysis and energy production processes impacting the cellular metabolism and biochemical energy cycles. The enzyme also contributes to gluconeogenesis where it helps synthesize glucose from non-carbohydrate sources ensuring energy supply during fasting.

Mutations or deficiencies in triosephosphate isomerase can lead to conditions such as triosephosphate isomerase deficiency a rare autosomal recessive disorder characterized by progressive neurological dysfunction hemolytic anemia and muscle weakness. This deficiency arises from dysfunctional or unstable isomerase activity leading to an accumulation of DHAP. Additionally research has shown links between this enzyme and Alzheimer's disease where altered glucose metabolism is a common feature. Abnormal triosephosphate isomerase activity may interact with amyloid precursor protein processing in the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.. It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.
See full target information TPI1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular and cellular cardiology 164:136-147 PubMed34923199

2021

A roadmap for the characterization of energy metabolism in human cardiomyocytes derived from induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Emanuelli,Anna Zoccarato,Christina M Reumiller,Angelos Papadopoulos,Mei Chong,Sabine Rebs,Kai Betteridge,Matteo Beretta,Katrin Streckfuss-Bömeke,Ajay M Shah

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 54:e9850 PubMed33656056

2021

Differential proteomic analysis of children infected with respiratory syncytial virus.

Applications

Unspecified application

Species

Unspecified reactive species

Gen-Quan Yin,Hui-Xuan Zeng,Zi-Long Li,Chen Chen,Jia-Yong Zhong,Mi-Si Xiao,Qiang Zeng,Wen-Hui Jiang,Pei-Qiong Wu,Jie-Min Zeng,Xiao-Yin Hu,Huan-Hui Chen, Ruo-Hu,Hai-Jin Zhao,Lin Gao,Cong Liu,Shao-Xi Cai

Endocrinology 162: PubMed33341896

2020

Endothelial Cell-Derived Triosephosphate Isomerase Attenuates Insulin Secretion From Pancreatic Beta Cells of Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Bareket Daniel,Ariela Livne,Guy Cohen,Shirin Kahremany,Shlomo Sasson

Frontiers in aging neuroscience 12:248 PubMed32973486

2020

Chronic Periodontitis and Alzheimer Disease: A Putative Link of Serum Proteins Identification by 2D-DIGE Proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Xianfang Rong,Liping Xiang,Yanfen Li,Hongfa Yang,Weijian Chen,Lei Li,Defeng Liang,Xincai Zhou

Archives of toxicology 93:1993-2006 PubMed31123803

2019

Proteomic analysis of hippocampal proteins in acrylamide-exposed Wistar rats.

Applications

Unspecified application

Species

Unspecified reactive species

Daichi Nagashima,Lingyi Zhang,Yuki Kitamura,Sahoko Ichihara,Eri Watanabe,Cai Zong,Yuko Yamano,Toshihiro Sakurai,Shinji Oikawa,Gaku Ichihara

Nature 548:112-116 PubMed28723898

2017

Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Song Zhang,Simon A Hawley,Yue Zong,Mengqi Li,Zhichao Wang,Alexander Gray,Teng Ma,Jiwen Cui,Jin-Wei Feng,Mingjiang Zhu,Yu-Qing Wu,Terytty Yang Li,Zhiyun Ye,Shu-Yong Lin,Huiyong Yin,Hai-Long Piao,D Grahame Hardie,Sheng-Cai Lin

Proteomics 16:70-9 PubMed26573124

2015

Analysis of the effect of a novel therapeutic for type 2 diabetes on the proteome of a muscle cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela A Young,Siobhán Leonard,Darren S D Martin,John B C Findlay

PloS one 10:e0116270 PubMed25643152

2015

Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ∆133p53α mimic.

Applications

WB

Species

Mouse

Sonal Sawhney,Kylie Hood,Alisha Shaw,Antony W Braithwaite,Richard Stubbs,Noelyn A Hung,Janice A Royds,Tania L Slatter

Laboratory investigation; a journal of technical m 94:557-68 PubMed24589856

2014

HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition.

Applications

Unspecified application

Species

Rat

Mari Ekman,Bengt Uvelius,Sebastian Albinsson,Karl Swärd

Journal of proteome research 11:2721-38 PubMed22416763

2012

Quantitative analysis of the detergent-insoluble brain proteome in frontotemporal lobar degeneration using SILAC internal standards.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas T Seyfried,Yair M Gozal,Laura E Donovan,Jeremy H Herskowitz,Eric B Dammer,Qiangwei Xia,Li Ku,Jianjun Chang,Duc M Duong,Howard D Rees,Deborah S Cooper,Jonathan D Glass,Marla Gearing,Malú G Tansey,James J Lah,Yue Feng,Allan I Levey,Junmin Peng
View all publications
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