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AB96696

Anti-Triosephosphate isomerase antibody

5

(1 Review)

|

(13 Publications)

Rabbit Polyclonal Triosephosphate isomerase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human TPI1 aa 150 to C-terminus.

View Alternative Names

TPI, TPI1, Triosephosphate isomerase, TIM, Methylglyoxal synthase, Triose-phosphate isomerase

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triosephosphate isomerase antibody (AB96696)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triosephosphate isomerase antibody (AB96696)

ab96696, at a 1/100 dilution, staining Triosephosphate isomerase in paraffin embedded SNU16 (human) by Immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Triosephosphate isomerase antibody (AB96696)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Triosephosphate isomerase antibody (AB96696)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Triosephosphate isomerase protein at cytoplasm using ab96696 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).
Scale bar = 10 µM

Western blot - Anti-Triosephosphate isomerase antibody (AB96696)
  • WB

Supplier Data

Western blot - Anti-Triosephosphate isomerase antibody (AB96696)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Triosephosphate isomerase antibody (ab96696) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 27 kDa

false

Western blot - Anti-Triosephosphate isomerase antibody (AB96696)
  • WB

Supplier Data

Western blot - Anti-Triosephosphate isomerase antibody (AB96696)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Triosephosphate isomerase antibody (ab96696) at 1/1000 dilution

Lane 1:

PC-12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 27 kDa

false

Western blot - Anti-Triosephosphate isomerase antibody (AB96696)
  • WB

Supplier Data

Western blot - Anti-Triosephosphate isomerase antibody (AB96696)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Triosephosphate isomerase antibody (ab96696) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Lane 3:

C8D30 whole cell lysate at 30 µg

Lane 4:

NIH-3T3 whole cell lysate at 30 µg

Lane 5:

BCL-1 whole cell lysate at 30 µg

Lane 6:

Raw 264.7 whole cell lysate at 30 µg

Lane 7:

C2Cl2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human TPI1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P60174

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Triosephosphate isomerase also known as TPI is an enzyme that plays an important role in glycolysis and gluconeogenesis by catalyzing the reversible isomerization of dihydroxyacetone phosphate (DHAP) to glyceraldehyde 3-phosphate (G3P). It has a molecular mass of approximately 27 kDa. This enzyme is widely expressed in many tissues with high presence in red blood cells muscle and brain tissues due to their high energy demands. As a dimer enzyme it exhibits significant catalytic efficiency that supports cellular energy management.
Biological function summary

The enzyme facilitates the efficient production of ATP by maintaining balance between DHAP and G3P vital for energy-metabolizing cells. Triosephosphate isomerase is not part of a larger complex itself but it significantly interacts with other enzymes in the glycolytic pathway. This interaction supports cellular respiration by ensuring a steady supply of intermediates needed for downstream processes.

Pathways

Triosephosphate isomerase is central to glycolysis and gluconeogenesis pathways. In glycolysis it works in tandem with other enzymes such as hexokinase and phosphofructokinase to breakdown glucose into pyruvate yielding ATP and NADH. It provides a critical link between glycolysis and energy production processes impacting the cellular metabolism and biochemical energy cycles. The enzyme also contributes to gluconeogenesis where it helps synthesize glucose from non-carbohydrate sources ensuring energy supply during fasting.

Mutations or deficiencies in triosephosphate isomerase can lead to conditions such as triosephosphate isomerase deficiency a rare autosomal recessive disorder characterized by progressive neurological dysfunction hemolytic anemia and muscle weakness. This deficiency arises from dysfunctional or unstable isomerase activity leading to an accumulation of DHAP. Additionally research has shown links between this enzyme and Alzheimer's disease where altered glucose metabolism is a common feature. Abnormal triosephosphate isomerase activity may interact with amyloid precursor protein processing in the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.. It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.
See full target information TPI1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Aging 17:1929-1965 PubMed40748658

2025

Exosomes released from senescent cells and circulatory exosomes isolated from human plasma reveal aging-associated proteomic and lipid signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Sandip Kumar Patel,Joanna Bons,Jacob P Rose,Jessie R Chappel,Rebecca L Beres,Mark A Watson,Corey Webster,Jordan B Burton,Roland Bruderer,Pierre-Yves Desprez,Lukas Reiter,Judith Campisi,Erin S Baker,Birgit Schilling

American journal of translational research 15:982-994 PubMed36915794

2023

Beta-catenin knockdown impairs the viability of ovarian cancer cells by modulating YAP-dependent glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Shuang Jia,Ling Yang,Yong-Qing Zhu,Cheng-Bin Ma

Cancer discovery 13:1002-1025 PubMed36715544

2023

LKB1-Dependent Regulation of TPI1 Creates a Divergent Metabolic Liability between Human and Mouse Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin D Stein,John R Ferrarone,Eric E Gardner,Jae Won Chang,David Wu,Pablo E Hollstein,Roger J Liang,Min Yuan,Qiuying Chen,John S Coukos,Miriam Sindelar,Bryan Ngo,Steven S Gross,Reuben J Shaw,Chen Zhang,John M Asara,Raymond E Moellering,Harold Varmus,Lewis C Cantley

Nature cell biology 23:1240-1254 PubMed34887515

2021

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Zhang,Dennis K Jeppesen,James N Higginbotham,Ramona Graves-Deal,Vincent Q Trinh,Marisol A Ramirez,Yoojin Sohn,Abigail C Neininger,Nilay Taneja,Eliot T McKinley,Hiroaki Niitsu,Zheng Cao,Rachel Evans,Sarah E Glass,Kevin C Ray,William H Fissell,Salisha Hill,Kristie Lindsey Rose,Won Jae Huh,Mary Kay Washington,Gregory Daniel Ayers,Dylan T Burnette,Shivani Sharma,Leonard H Rome,Jeffrey L Franklin,Youngmin A Lee,Qi Liu,Robert J Coffey

Scientific reports 11:7023 PubMed33782423

2021

STAT3 governs the HIF-1α response in IL-15 primed human NK cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Coulibaly,Sonia Y Velásquez,Nina Kassner,Jutta Schulte,Maria Vittoria Barbarossa,Holger A Lindner

Cell death and differentiation 28:1091-1109 PubMed33082514

2020

The deubiquitinase JOSD2 is a positive regulator of glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Lyudmila Krassikova,Boxi Zhang,Divya Nagarajan,André Lima Queiroz,Merve Kacal,Evangelos Samakidis,Helin Vakifahmetoglu-Norberg,Erik Norberg

Cell 182:641-654.e20 PubMed32615085

2020

Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Wu,Kate E R Hollinshead,Yuhan Hao,Christy Au,Lina Kroehling,Charles Ng,Woan-Yu Lin,Dayi Li,Hernandez Moura Silva,Jong Shin,Juan J Lafaille,Richard Possemato,Michael E Pacold,Thales Papagiannakopoulos,Alec C Kimmelman,Rahul Satija,Dan R Littman

Cells 9: PubMed32192004

2020

Innate Cytokine Induced Early Release of IFNγ and CC Chemokines from Hypoxic Human NK Cells Is Independent of Glucose.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Y Velásquez,Bianca S Himmelhan,Nina Kassner,Anna Coulibaly,Jutta Schulte,Kathrin Brohm,Holger A Lindner

PLoS biology 17:e3000425 PubMed31461438

2019

Targeting oxidative pentose phosphate pathway prevents recurrence in mutant Kras colorectal carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

WenChao Gao,YuTing Xu,Tao Chen,ZunGuo Du,XiuJuan Liu,ZhiQian Hu,Dong Wei,ChunFang Gao,Wei Zhang,QingQuan Li

Journal of cellular physiology 233:8919-8929 PubMed29856481

2018

Triose-phosphate isomerase is a novel target of miR-22 and miR-28, with implications in tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Saife Niaz Lone,Raihana Maqbool,Fazl Q Parray,Mahboob Ul Hussain
View all publications
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