Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell) cells labelling TRIP12/ULF with ab313628 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TRIP12/ULF with ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Low expression : weakly nuclear staining on human liver. The section was incubated with ab313628 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling TRIP12/ULF with ab313628 at 1/50 (9.82 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in HeLa cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TRIP12/ULF with ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nuclear staining on human testis. The section was incubated with ab313628 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling TRIP12/ULF with ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nuclear staining on human lung cancer. The section was incubated with ab313628 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Flow cytometric analysis of C2C12 (mouse myoblast) cells labelling TRIP12/ULF with ab313628 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling TRIP12/ULF with ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Low expression : weakly nuclear staining on mouse liver. The section was incubated with ab313628 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling TRIP12/ULF with ab313628 at 1/50 (9.82 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in C2C12 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling TRIP12/ULF with ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID : 23663701). The section was incubated with ab313628 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling TRIP12/ULF with ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis. The section was incubated with ab313628 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• WB

Supplier Data

Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 59 seconds

All lanes:

Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] (<a href='/en-us/products/primary-antibodies/trip12-ulf-antibody-epr27062-86-ab313628'>ab313628</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

Hela transfected with siRNA specifically targeti TRIP12/ULF whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 220 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : Kidney (PMID : 23663701). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 23663701). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 59 seconds

All lanes:

Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] (<a href='/en-us/products/primary-antibodies/trip12-ulf-antibody-epr27062-86-ab313628'>ab313628</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 220 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 48 seconds

All lanes:

Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] (<a href='/en-us/products/primary-antibodies/trip12-ulf-antibody-epr27062-86-ab313628'>ab313628</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 5:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 220 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629)

This data was developed using ab313628, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : brain, liver (PMID : 23663701). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 23663701). Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] (<a href='/en-us/products/primary-antibodies/trip12-ulf-antibody-epr27062-86-ab313628'>ab313628</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Lane 6:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 220 kDa

false

Exposure time: 15s