Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)

Rabbit Recombinant Monoclonal TRIP12/ULF antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (AB313629), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt (Intra)	WB	ICC/IF	IP
Human	Tested	Tested	Tested	Tested	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended
Rat	Tested	Expected	Tested	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information TRIP12

Function

E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair (PubMed:19028681, PubMed:22884692). Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardless of the presence of lysine residues in target proteins (PubMed:19028681). Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes (PubMed:22884692). In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress (PubMed:20208519). In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation (PubMed:20208519). Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A (PubMed:20208519). Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation (PubMed:18627766). Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins (PubMed:20829358). Mediates ubiquitination of ASXL1: following binding to N(6)-methyladenosine methylated DNA, ASXL1 is ubiquitinated by TRIP12, leading to its degradation and subsequent inactivation of the PR-DUB complex (PubMed:30982744).

Alternative names

KIAA0045, ULF, TRIP12, E3 ubiquitin-protein ligase TRIP12, E3 ubiquitin-protein ligase for Arf, HECT-type E3 ubiquitin transferase TRIP12, Thyroid receptor-interacting protein 12, TR-interacting protein 12, TRIP-12

Recommended products

Rabbit Recombinant Monoclonal TRIP12/ULF antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR27062-86
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TRIP12 also known as Thyroid Hormone Receptor Interactor 12 or ULF (Ubiquitin Ligase for FBW7) is an E3 ubiquitin ligase. Mechanically TRIP12 facilitates the ubiquitination and subsequent proteasomal degradation of specific proteins. It has an approximate mass of 220 kDa. This protein is expressed in various tissues including the brain and liver. TRIP12 often interacts with other proteins through its ARM and HECT domains playing an important role in regulating protein stability.

Biological function summary

TRIP12 functions as a regulator of protein turnover impacting processes such as cell cycle progression and DNA damage responses. It is not part of a larger complex but operates independently to exert its influence. The target specifically targets proteins like p53 and FBW7 for degradation allowing cells to maintain homeostasis and adapt to changing conditions. This regulation ensures cells respond appropriately to DNA damage by either promoting repair pathways or facilitating apoptosis when repair is not feasible.

Pathways

TRIP12 plays a role in the ubiquitin-proteasome system and the p53 signaling pathway. In the ubiquitin-proteasome system TRIP12 works alongside other E3 ligases to regulate protein degradation ensuring proteins that are damaged or no longer needed are efficiently removed. Within the p53 signaling pathway TRIP12 influences the stability and activity of the tumor suppressor protein p53 helping control cell cycle arrest and apoptosis. TRIP12's relationship with FBW7 further connects it to pathways involved in cancer as FBW7 targets many oncoproteins.

Associated diseases and disorders

TRIP12 has links to cancer and neurodevelopmental disorders. Alterations in the activity or expression of TRIP12 can lead to the accumulation of proteins like p53 which may contribute to tumorigenesis. Mutations in TRIP12 have also been associated with neurodevelopmental disorders where disrupted protein turnover impacts neural development. Its interaction with proteins such as p53 and FBW7 highlights its potential role in disease mechanisms making it a target of interest for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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14 product images

Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: brain, liver (PMID: 23663701).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23663701).

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 15 seconds
All lanes: Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] (Anti-TRIP12/ULF antibody [EPR27062-86] ab313628) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Mouse testis tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat liver tissue lysate at 20 µg
Lane 6: Rat testis tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 220 kDa
Exposure time: 15s
Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: Kidney (PMID: 23663701).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23663701).

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 59 seconds
All lanes: Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] (Anti-TRIP12/ULF antibody [EPR27062-86] ab313628) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 220 kDa
Exposure time: 59s
Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Exposure time: 48 seconds
All lanes: Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] (Anti-TRIP12/ULF antibody [EPR27062-86] ab313628) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Lane 5: C2C12 (mouse myoblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 220 kDa
Exposure time: 48s
Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 59 seconds
All lanes: Western blot - Anti-TRIP12/ULF antibody [EPR27062-86] (Anti-TRIP12/ULF antibody [EPR27062-86] ab313628) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: Hela transfected with siRNA specifically targeti TRIP12/ULF whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 220 kDa
Exposure time: 59s
Flow Cytometry (Intracellular) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of C2C12 (mouse myoblast) cells labelling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell) cells labelling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in C2C12 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in HeLa cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Low expression: weakly nuclear staining on mouse liver. The section was incubated with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Low expression: weakly nuclear staining on human liver. The section was incubated with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on rat testis. The section was incubated with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on mouse testis (PMID: 23663701). The section was incubated with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Nuclear staining on human lung cancer. The section was incubated with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP12/ULF antibody [EPR27062-86] - BSA and Azide free (ab313629)
This data was developed using Anti-TRIP12/ULF antibody [EPR27062-86] ab313628, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Nuclear staining on human testis. The section was incubated with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)