AB128153

Anti-TRIP13/PCH2 antibody

Name: Anti-TRIP13/PCH2 antibody (ab128153) | Abcam
Brand: Abcam Limited
SKU: AB128153
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal TRIP13/PCH2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human TRIP13 aa 1-50.

View Alternative Names

PCH2, TRIP13, Pachytene checkpoint protein 2 homolog, Human papillomavirus type 16 E1 protein-binding protein, Thyroid hormone receptor interactor 13, Thyroid receptor-interacting protein 13, 16E1-BP, HPV16 E1 protein-binding protein, TR-interacting protein 13, TRIP-13

2 Images

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Immunoprecipitation - Anti-TRIP13/PCH2 antibody (AB128153)

Detection of TRIP13/PCH2 by Western Blot of Immunprecipitate.
ab128153 at 1μg/ml staining TRIP13/PCH2 in HeLa whole cell lysate immunoprecipitated using ab128153 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-TRIP13/PCH2 antibody (ab128153)

Predicted band size: 49 kDa

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Unknown

Western blot - Anti-TRIP13/PCH2 antibody (AB128153)

All lanes:

Western blot - Anti-TRIP13/PCH2 antibody (ab128153) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 49 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TRIP13 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q15645

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Opossum": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab128153 was affinity purified using an epitope specific to TRIP13/PCH2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIP13 also known as PCH2 is a protein involved in the regulation of chromosome dynamics during meiosis and mitosis. It is approximately 48 kDa in size. TRIP13 plays an important role in promoting the disassembly of the synaptonemal complex and ensuring proper chromosome segregation. This protein is expressed in various tissues but shows higher levels in testis suggesting its importance in reproductive cell division.

Biological function summary

TRIP13 contributes to efficient cell cycle progression by participating in the spindle assembly checkpoint (SAC). It forms part of the mitotic checkpoint complex ensuring that chromosomes are correctly attached to the spindle microtubules before progression through mitosis. TRIP13 works in conjunction with other proteins like MAD2 and BUB1 to maintain genomic stability.

Pathways

TRIP13 is involved in the cell cycle control and DNA repair pathways. It influences the mitotic spindle checkpoint interacting with proteins such as MAD2 which ensures that cells do not proceed to anaphase until all chromosomes reach proper alignment. TRIP13 also plays a role in homologous recombination repair by regulating the processing of recombination intermediates.

TRIP13 has been implicated in cancer and infertility. Its overexpression or mutation has been linked to tumor progression and resistance to certain chemotherapeutic agents due to its role in chromosome segregation and genomic stability. Additionally TRIP13 dysfunction may contribute to infertility through its impact on meiosis with potential interactions with proteins involved in reproductive processes such as SYCP3.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double-strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes (By similarity). Plays a role in mitotic spindle assembly checkpoint (SAC) activation (PubMed : 28553959).

See full target information TRIP13

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9614 PubMed39511190

2024

The BAF complex enhances transcription through interaction with H3K56ac in the histone globular domain.

Applications

Unspecified application

Species

Unspecified reactive species

Kwangbeom Hyun,Jihye Ahn,Hyoungmin Kim,Jihyun Kim,Yong-In Kim,Hee-Sung Park,Robert G Roeder,J Eugene Lee,Jaehoon Kim

International journal of molecular sciences 24: PubMed37958783

2023

REV7 Monomer Is Unable to Participate in Double Strand Break Repair and Translesion Synthesis but Suppresses Mitotic Errors.

Applications

Unspecified application

Species

Unspecified reactive species

Faye M Vassel,Daniel J Laverty,Ke Bian,Cortt G Piett,Michael T Hemann,Graham C Walker,Zachary D Nagel

Oncology letters 23:112 PubMed35251343

2022

KIF18B promotes breast cancer cell proliferation, migration and invasion by targeting TRIP13 and activating the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Liu,Zhaofeng Zhang,Xiulin Xia,Jing Lei

JCI insight 6: PubMed34806647

2021

DNA damage is overcome by TRIP13 overexpression during cisplatin nephrotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Taketsugu Hama,Prashanth Kb Nagesh,Pallabita Chowdhury,Bob M Moore,Murali M Yallapu,Kevin R Regner,Frank Park

Nature communications 12:5421 PubMed34521823

2021

MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Inge de Krijger,Bastian Föhr,Santiago Hernández Pérez,Estelle Vincendeau,Judit Serrat,Alexander Marc Thouin,Vivek Susvirkar,Chloé Lescale,Inés Paniagua,Liesbeth Hoekman,Simranjeet Kaur,Maarten Altelaar,Ludovic Deriano,Alex C Faesen,Jacqueline J L Jacobs

American journal of human genetics 107:15-23 PubMed32473092

2020

Bi-allelic Missense Pathogenic Variants in TRIP13 Cause Female Infertility Characterized by Oocyte Maturation Arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihua Zhang,Bin Li,Jing Fu,Rong Li,Feiyang Diao,Caihong Li,Biaobang Chen,Jing Du,Zhou Zhou,Jian Mu,Zheng Yan,Ling Wu,Shuai Liu,Wenjing Wang,Lin Zhao,Jie Dong,Lin He,Xiaozhen Liang,Yanping Kuang,Xiaoxi Sun,Qing Sang,Lei Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TRIP13/PCH2 antibody

Immunoprecipitation - Anti-TRIP13/PCH2 antibody (AB128153)

Western blot - Anti-TRIP13/PCH2 antibody (AB128153)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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