Anti-TRIP13/PCH2 antibody

• IP

Unknown

Immunoprecipitation - Anti-TRIP13/PCH2 antibody (AB128171)

Detection of TRIP13/PCH2 by Western Blot of Immunprecipitate.
a128171, at 1 μg/ml, staining TRIP13/PCH2 in HeLa whole cell lysate immunoprecipitated using ab128171 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG.
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TRIP13/PCH2 antibody (ab128171)

Predicted band size: 49 kDa

false

• WB

Unknown

Western blot - Anti-TRIP13/PCH2 antibody (AB128171)

All lanes:

Western blot - Anti-TRIP13/PCH2 antibody (ab128171) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 49 kDa

true

Exposure time: 3min

• WB

Lab

Western blot - Anti-TRIP13/PCH2 antibody (AB128171)

Anti-TRIP13 antibody (ab128171) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab128171 was shown to bind specifically to TRIP13. A band was observed at 48 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TRIP13 knockout cell line. To generate this image, wild-type and TRIP13 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-TRIP13/PCH2 antibody (ab128171) at 1/2000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human TRIP13 (PCH2) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-trip13-pch2-knockout-hek-293t-cell-line-ab266495'>ab266495</a>)

Lane 2:

TRIP13 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human TRIP13 (PCH2) knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-trip13-pch2-knockout-hek-293t-cell-lysate-ab258736'>ab258736</a>)

Lane 3:

HCT 116 cell lysate at 20 µg

Lane 4:

SW480 cell lysate at 20 µg

Predicted band size: 49 kDa

Observed band size: 48 kDa

false