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Knockout Tested Rabbit Recombinant Monoclonal TRIP13/PCH2 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Rat samples.

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Images

Immunoprecipitation - Anti-TRIP13/PCH2 antibody [EPR27063-80] - BSA and Azide free (AB313703), expandable thumbnail
  • Western blot - Anti-TRIP13/PCH2 antibody [EPR27063-80] - BSA and Azide free (AB313703), expandable thumbnail
  • Western blot - Anti-TRIP13/PCH2 antibody [EPR27063-80] - BSA and Azide free (AB313703), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)ICC/IFIHC-P
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double-strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes (By similarity). Plays a role in mitotic spindle assembly checkpoint (SAC) activation (PubMed:28553959).

Alternative names

PCH2, TRIP13, Pachytene checkpoint protein 2 homolog, Human papillomavirus type 16 E1 protein-binding protein, Thyroid hormone receptor interactor 13, Thyroid receptor-interacting protein 13, 16E1-BP, HPV16 E1 protein-binding protein, TR-interacting protein 13, TRIP-13

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal TRIP13/PCH2 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27063-80
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TRIP13 also known as PCH2 is a protein involved in the regulation of chromosome dynamics during meiosis and mitosis. It is approximately 48 kDa in size. TRIP13 plays an important role in promoting the disassembly of the synaptonemal complex and ensuring proper chromosome segregation. This protein is expressed in various tissues but shows higher levels in testis suggesting its importance in reproductive cell division.

Biological function summary

TRIP13 contributes to efficient cell cycle progression by participating in the spindle assembly checkpoint (SAC). It forms part of the mitotic checkpoint complex ensuring that chromosomes are correctly attached to the spindle microtubules before progression through mitosis. TRIP13 works in conjunction with other proteins like MAD2 and BUB1 to maintain genomic stability.

Pathways

TRIP13 is involved in the cell cycle control and DNA repair pathways. It influences the mitotic spindle checkpoint interacting with proteins such as MAD2 which ensures that cells do not proceed to anaphase until all chromosomes reach proper alignment. TRIP13 also plays a role in homologous recombination repair by regulating the processing of recombination intermediates.

Associated diseases and disorders

TRIP13 has been implicated in cancer and infertility. Its overexpression or mutation has been linked to tumor progression and resistance to certain chemotherapeutic agents due to its role in chromosome segregation and genomic stability. Additionally TRIP13 dysfunction may contribute to infertility through its impact on meiosis with potential interactions with proteins involved in reproductive processes such as SYCP3.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com