AB137478

Anti-TRIP6 antibody

Name: Anti-TRIP6 antibody (ab137478) | Abcam
Brand: Abcam Limited
SKU: AB137478

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(3 Publications)

Rabbit Polyclonal TRIP6 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIP6 aa 150 to C-terminus.

View Alternative Names

OIP1, TRIP6, Thyroid receptor-interacting protein 6, TR-interacting protein 6, TRIP-6, Opa-interacting protein 1, Zyxin-related protein 1, OIP-1, ZRP-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP6 antibody (AB137478)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP6 antibody (AB137478)

Immunohistochemical analysis of paraffin embedded Human TOV21G xenograft tissue labelling TRIP6 with ab137478 at 1/500 dilution.

Supplier Data

Western blot - Anti-TRIP6 antibody (AB137478)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-TRIP6 antibody (ab137478) at 1/500 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-TRIP6 antibody (AB137478)

Samples were separated by 10% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-TRIP6 antibody (ab137478) at 1/1000 dilution

Lane 1:

AsPC-1 whole cell extracts at 30 µg

Lane 2:

BxPC3 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TRIP6 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15654

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIP6 also known as Thyroid Hormone Receptor Interactor 6 or ZRP-1 (Zyxin Related Protein 1) is a cytoskeletal adaptor protein with a molecular mass of approximately 55 kDa. Its primary function involves acting as a scaffold protein that facilitates interactions between several intracellular signaling molecules. TRIP6 localizes mainly to focal adhesion sites and the actin cytoskeleton where it regulates cell motility and organization. This protein is usually expressed in various tissues but expression levels are notably prominent in the lymphoid tissues and the gastrointestinal tract.

Biological function summary

The mechanical interactions of TRIP6 influence cell migration adhesion and morphogenesis. It functions as a component of the focal adhesion complex where it plays an important role in linking the actin cytoskeleton to integrin receptors. Additionally TRIP6 participates in the regulation of gene expression by translocating to the nucleus and interacting with transcription factors to modulate specific gene targets.

Pathways

The involvement of TRIP6 is significant within signaling pathways such as the RhoA pathway and the NF-kB signaling pathway. Through RhoA TRIP6 contributes to the modulation of cell shape and motility impacting cellular processes such as angiogenesis and wound healing. In the NF-kB pathway TRIP6 can influence inflammatory responses by affecting gene transcription. TRIP6 is associated with proteins like paxillin and focal adhesion kinase (FAK) which are key components in these pathways.

TRIP6 has notable implications in cancer progression and inflammatory diseases. It has been implicated as a contributor to tumorigenesis due to its role in cell motility and invasion often showing altered expression in various cancers such as breast and prostate cancer. TRIP6 also interacts with the protein zyxin and this interaction influences cancer cell metastasis. Additionally TRIP6 can affect inflammatory responses in disorders such as inflammatory bowel disease where its modulation impacts cellular pathways related to inflammation and immune response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor.

See full target information TRIP6

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 73:69 PubMed38430268

2024

Construction of an immune predictive model and identification of TRIP6 as a prognostic marker and therapeutic target of CRC by integration of single-cell and bulk RNA-seq data.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Liu,Xitu Luo,Zilang Zhang,Yepeng Chen,Yongliang Dai,Jianzhong Deng,Chengyu Yang,Hao Liu

Cancer cell international 20:51 PubMed32082081

2020

TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Zhao,Chao Jiang,Rui Xu,Qingnan Liu,Guanglin Liu,Yan Zhang

Biologics : targets & therapy 13:101-110 PubMed31354238

2019

TRIP6 functions as a potential oncogene and facilitated proliferation and metastasis of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lirong Zhu,Xiaodong Xu,Yijie Tang,Xiaochao Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIP6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP6 antibody (AB137478)

Western blot - Anti-TRIP6 antibody (AB137478)

Western blot - Anti-TRIP6 antibody (AB137478)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise