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AB250179

Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Tripeptidyl peptidase II/TPPII antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Tripeptidyl-peptidase 2, TPP-2, Tripeptidyl aminopeptidase, Tripeptidyl-peptidase II, TPP-II, TPP2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (AB250179)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (AB250179)

This data was developed using ab180177, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/100 (red) and DAPI staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (AB250179)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (AB250179)

This data was developed using ab180177, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (AB250179)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (AB250179)

This data was developed using ab180177, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green).

Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (AB250179)
  • WB

Supplier Data

Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (AB250179)

This data was developed using ab180177, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (<a href='/en-us/products/primary-antibodies/tripeptidyl-peptidase-ii-tppii-antibody-epr13202-ab180177'>ab180177</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Fetal liver tissue lysate at 10 µg

Predicted band size: 138 kDa

Observed band size: 138 kDa

false

  • Unconjugated

    Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13202

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250179 is the carrier-free version of ab180177.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tripeptidyl peptidase II (TPPII) also known as TPP2 is a large peptidase complex that mechanistically degrades oligopeptides into tripeptides. It consists of a massive molecular complex with a molecular mass of approximately 5-6 MDa. TPPII is ubiquitously expressed in various tissues with high expression levels in the liver muscle and kidney. This complex is cytosolic and possesses unique endopeptidase and exopeptidase activities which makes it distinct in both structure and function from the proteasome.
Biological function summary

TPPII is an enzyme that plays a vital role in the regulation of intracellular protein turnover and antigen processing. It contributes to protein degradation especially when proteasome activities are inhibited or overwhelmed. TPPII operates in a complex and involves the dynamic assembly of its subunits to form a functional peptidase complex. Through its action TPPII maintains protein homeostasis and assists in generating peptides presented by MHC class I molecules important for immune surveillance.

Pathways

TPPII functions within the ubiquitin-proteasome system and the antigen presentation pathway. It acts as a backup to the proteasome ensuring continued protein degradation. TPPII interacts with proteins such as ubiquitin and E3 ligases which tag proteins for degradation. During antigen processing TPPII complements proteasome activity by processing longer peptides into shorter ones suitable for MHC class I presentation thereby interfacing with molecules like TAP and the proteasome subunits.

Dysregulation of TPPII is associated with conditions such as cancer and neurodegenerative diseases. The upregulation of TPPII has been observed in several tumors implicating its role in promoting cell survival and proliferation. In cancer TPPII may interact with proteins such as p53 affecting apoptosis and cell cycle regulation. In neurodegenerative diseases altered TPPII function might influence protein aggregation and degradation pathways where it might relate with proteins like tau and amyloid-beta contributing to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytosolic tripeptidyl-peptidase that releases N-terminal tripeptides from polypeptides and is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway (PubMed : 25525876, PubMed : 30533531). It plays an important role in intracellular amino acid homeostasis (PubMed : 25525876). Stimulates adipogenesis (By similarity).
See full target information Tripeptidyl-peptidase 2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang
View all publications
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