AB150428

Anti-TRK fused gene antibody [EPR8765(2)(B)]

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal TRK fused gene antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

Protein TFG, TRK-fused gene protein, TFG

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TRK fused gene with ab150428 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunofluorescent analysis of HeLa cells labelling TRK fused gene with ab150428 at 1/250 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

All lanes:

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 43 kDa

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Lab

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Lanes 1-4 : Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa.

ab150428 Anti-TRK fused gene antibody [EPR8765(2)(B)] was shown to specifically react with TFG wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265841 (knockout cell lysate ab257738) was used. Wild-type and TFG knockout samples were subjected to SDS-PAGE. ab150428 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TFG knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TFG (TRK fused gene) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tfg-trk-fused-gene-knockout-hela-cell-line-ab265841'>ab265841</a>)

Lane 3:

HAP-1 cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 57 kDa

false

Lab

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Lanes 1 - 4 : Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab150428 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween^®) before incubation with ab150428 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/10000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

TFG knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

MCF7 whole cell lysate at 20 µg

Predicted band size: 43 kDa

Observed band size: 57 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8765(2)(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TRK fused gene also known as NTRK or tropomyosin receptor kinase encodes for a family of proteins integral to neurotrophin signaling. These proteins weighing approximately 80-90 kilodaltons include isoforms such as TRKA TRKB and TRKC which are products of the NTRK1 NTRK2 and NTRK3 genes respectively. They express mainly in neuronal tissues and certain non-neuronal cells. These proteins possess a diverse range of functions largely due to structural variations derived from gene fusions.

Biological function summary

Neurotrophin signaling pathways involve TRK proteins facilitating neuronal differentiation survival and plasticity. TRK proteins often form part of larger complexes engaged in transmitting signals from the extracellular environment to intracellular pathways. The TRK proteins interact with neurotrophins like NGF BDNF and NT-3 playing essential roles in nervous system function and development. The interaction between TRK receptors and their ligands activates downstream signaling cascades.

Pathways

TRK proteins significantly contribute to the MAPK/ERK and PI3K/Akt pathways. These pathways control cell growth differentiation and survival. Within these pathways TRK proteins interact with partners such as Ras and Akt coordinating a broad range of cellular processes. They ensure proper cellular responses to external stimuli and maintain homeostasis within the cell.

TRK fusion proteins frequently associate with certain cancers including thyroid and lung cancer due to gene fusions leading to overactive signaling. Altered TRK signaling correlates with neurodegenerative diseases as well such as Alzheimer's disease. TRK fusions can affect proteins like ETV6 and LMNA in the context of cancer influencing tumorigenesis and disease progression. Understanding the connections between TRK fusions and these diseases aids the development of target-specific therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the normal dynamic function of the endoplasmic reticulum (ER) and its associated microtubules (PubMed : 23479643, PubMed : 27813252). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed : 21478858).

See full target information Protein TFG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical research 37:418-430 PubMed37977559

2023

Galectin-14 promotes hepatocellular carcinoma tumor growth enhancing heparan sulfate proteoglycan modification.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Gou,Gang Yang,Sujuan Ma,Tong Ding,Luan Sun,Fang Liu,Jin Huang,Wei Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRK fused gene antibody [EPR8765(2)(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise