Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

Rabbit Recombinant Monoclonal TRK fused gene antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples.

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Images

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF
Human	Tested	Not recommended	Tested	Tested
Mouse	Expected	Not recommended	Expected	Expected
Rat	Predicted	Not recommended	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50 - 1/100	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50 - 1/100	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250 - 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250 - 1/500	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information Protein TFG

Function

Plays a role in the normal dynamic function of the endoplasmic reticulum (ER) and its associated microtubules (PubMed:23479643, PubMed:27813252). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed:21478858).

Alternative names

Protein TFG, TRK-fused gene protein, TFG

Recommended products

Rabbit Recombinant Monoclonal TRK fused gene antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR8765(2)(B)
Purity: Tissue culture supernatant
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The TRK fused gene also known as NTRK or tropomyosin receptor kinase encodes for a family of proteins integral to neurotrophin signaling. These proteins weighing approximately 80-90 kilodaltons include isoforms such as TRKA TRKB and TRKC which are products of the NTRK1 NTRK2 and NTRK3 genes respectively. They express mainly in neuronal tissues and certain non-neuronal cells. These proteins possess a diverse range of functions largely due to structural variations derived from gene fusions.

Biological function summary

Neurotrophin signaling pathways involve TRK proteins facilitating neuronal differentiation survival and plasticity. TRK proteins often form part of larger complexes engaged in transmitting signals from the extracellular environment to intracellular pathways. The TRK proteins interact with neurotrophins like NGF BDNF and NT-3 playing essential roles in nervous system function and development. The interaction between TRK receptors and their ligands activates downstream signaling cascades.

Pathways

TRK proteins significantly contribute to the MAPK/ERK and PI3K/Akt pathways. These pathways control cell growth differentiation and survival. Within these pathways TRK proteins interact with partners such as Ras and Akt coordinating a broad range of cellular processes. They ensure proper cellular responses to external stimuli and maintain homeostasis within the cell.

Associated diseases and disorders

TRK fusion proteins frequently associate with certain cancers including thyroid and lung cancer due to gene fusions leading to overactive signaling. Altered TRK signaling correlates with neurodegenerative diseases as well such as Alzheimer's disease. TRK fusions can affect proteins like ETV6 and LMNA in the context of cancer influencing tumorigenesis and disease progression. Understanding the connections between TRK fusions and these diseases aids the development of target-specific therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
Lanes 1-4: Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab150428 Anti-TRK fused gene antibody [EPR8765(2)(B)] was shown to specifically react with TFG wild-type HeLa cells. Loss of signal was observed when knockout cell line Human TFG (TRK fused gene) knockout HeLa cell line ab265841 (knockout cell lysate Human TFG (TRK fused gene) knockout HeLa cell lysate ab257738) was used. Wild-type and TFG knockout samples were subjected to SDS-PAGE. ab150428 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: TFG knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human TFG (TRK fused gene) knockout HeLa cell line (Human TFG (TRK fused gene) knockout HeLa cell line ab265841)
Lane 3: HAP-1 cell lysate at 20 µg
Lane 4: MCF7 cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TRK fused gene with ab150428 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
Immunofluorescent analysis of HeLa cells labelling TRK fused gene with ab150428 at 1/250 dilution.
Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
Lanes 1 - 4: Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab150428 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween^®) before incubation with ab150428 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/10000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: TFG knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: MCF7 whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
All lanes: Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Secondary
All lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 43 kDa