Rabbit Recombinant Monoclonal TRK fused gene antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples.
Images
![Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (AB235872), expandable thumbnail](https://content.abcam.com/products/images/trk-fused-gene-antibody-epr87652b-bsa-and-azide-free-ab235872--western-blot-img645.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (AB235872)")
![Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (AB235872), expandable thumbnail](https://content.abcam.com/products/images/trk-fused-gene-antibody-epr87652b-bsa-and-azide-free-ab235872--western-blot-img139831.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (AB235872)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Expected | Expected |
| Mouse | Not recommended | Predicted | Expected | Expected |
| Rat | Not recommended | Predicted | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Plays a role in the normal dynamic function of the endoplasmic reticulum (ER) and its associated microtubules (PubMed:23479643, PubMed:27813252). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed:21478858).
Alternative names
Protein TFG, TRK-fused gene protein, TFG
Recommended products
Rabbit Recombinant Monoclonal TRK fused gene antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8765(2)(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab235872 is the carrier-free version of Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TRK fused gene also known as NTRK or tropomyosin receptor kinase encodes for a family of proteins integral to neurotrophin signaling. These proteins weighing approximately 80-90 kilodaltons include isoforms such as TRKA TRKB and TRKC which are products of the NTRK1 NTRK2 and NTRK3 genes respectively. They express mainly in neuronal tissues and certain non-neuronal cells. These proteins possess a diverse range of functions largely due to structural variations derived from gene fusions.
- Biological function summary
Neurotrophin signaling pathways involve TRK proteins facilitating neuronal differentiation survival and plasticity. TRK proteins often form part of larger complexes engaged in transmitting signals from the extracellular environment to intracellular pathways. The TRK proteins interact with neurotrophins like NGF BDNF and NT-3 playing essential roles in nervous system function and development. The interaction between TRK receptors and their ligands activates downstream signaling cascades.
- Pathways
TRK proteins significantly contribute to the MAPK/ERK and PI3K/Akt pathways. These pathways control cell growth differentiation and survival. Within these pathways TRK proteins interact with partners such as Ras and Akt coordinating a broad range of cellular processes. They ensure proper cellular responses to external stimuli and maintain homeostasis within the cell.
- Associated diseases and disorders
TRK fusion proteins frequently associate with certain cancers including thyroid and lung cancer due to gene fusions leading to overactive signaling. Altered TRK signaling correlates with neurodegenerative diseases as well such as Alzheimer's disease. TRK fusions can affect proteins like ETV6 and LMNA in the context of cancer influencing tumorigenesis and disease progression. Understanding the connections between TRK fusions and these diseases aids the development of target-specific therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872), expandable thumbnail](https://content.abcam.com/products/images/trk-fused-gene-antibody-epr87652b-bsa-and-azide-free-ab235872--western-blot-img645.jpg "Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)")
Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)
This data was developed using the same antibody clone in a different buffer formulation (Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428).
Lanes 1-4: Merged signal (red and green). Green - Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428 observed at 57 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428 Anti-TRK fused gene antibody [EPR8765(2)(B)] was shown to specifically react with TFG in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human TFG (TRK fused gene) knockout HeLa cell line ab265841 (knockout cell lysate Human TFG (TRK fused gene) knockout HeLa cell lysate ab257738) was used. Wild-type and TFG knockout samples were subjected to SDS-PAGE. Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: TFG knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human TFG (TRK fused gene) knockout HeLa cell line (Human TFG (TRK fused gene) knockout HeLa cell line ab265841)
Lane 3: HAP-1 cell lysate at 20 µg
Lane 4: MCF7 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 57 kDa
![Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872), expandable thumbnail](https://content.abcam.com/products/images/trk-fused-gene-antibody-epr87652b-bsa-and-azide-free-ab235872--western-blot-img139831.jpg "Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)")
Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)
This data was developed using the same antibody clone in a different buffer formulation (Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428 observed at 57 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (Anti-TRK fused gene antibody [EPR8765(2)(B)] ab150428) at 1/10000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: TFG knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: MCF7 whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 57 kDa
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