Anti-TRK fused gene antibody [EPR8766] - BSA and Azide free

• WB

Unknown

Western blot - Anti-TRK fused gene antibody [EPR8766] - BSA and Azide free (AB235871)

This data was developed using the same antibody clone in a different buffer formulation (ab156866).

Lanes 1-4 : Merged signal (red and green). Green - ab156866 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa.

ab156866 Anti-TRK fused gene antibody [EPR8766] was shown to specifically react with TFG in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265841 (knockout cell lysate ab257738) was used. Wild-type and TFG knockout samples were subjected to SDS-PAGE. ab156866 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRK fused gene antibody [EPR8766] (<a href='/en-us/products/primary-antibodies/trk-fused-gene-antibody-epr8766-ab156866'>ab156866</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TFG knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TFG (TRK fused gene) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tfg-trk-fused-gene-knockout-hela-cell-line-ab265841'>ab265841</a>)

Lane 3:

HAP-1 cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 57 kDa

false

• WB

Lab

Western blot - Anti-TRK fused gene antibody [EPR8766] - BSA and Azide free (AB235871)

This data was developed using the same antibody clone in a different buffer formulation (ab156866).

Lanes 1 - 4 : Merged signal (red and green). Green - ab156866 observed at 57 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab156866 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween^®) before incubation with ab156866 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRK fused gene antibody [EPR8766] (<a href='/en-us/products/primary-antibodies/trk-fused-gene-antibody-epr8766-ab156866'>ab156866</a>) at 1/10000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

TFG knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

MCF7 whole cell lysate at 20 µg

Predicted band size: 43 kDa

Observed band size: 57 kDa

false