AB197071

Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal TrkB phospho Y516 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Rat, Human samples. Cited in 6 publications.

View Alternative Names

TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B

5 Images

Supplier Data

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (AB197071)

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID : 23115189 and 20064930).

All lanes:

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/2000 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes at 20 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 140 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (AB197071)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution

Lane 1:

Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 2:

PC-12 treated with 100 ng/mL NGF (<a href='/en-us/products/proteins-peptides/recombinant-human-ngf-protein-active-ab9796'>ab9796</a>) for 5 minutes, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 140 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (AB197071)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution

Lane 1:

Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 2:

PC-12 treated with 100 ng/mL NGF (<a href='/en-us/products/proteins-peptides/recombinant-human-ngf-protein-active-ab9796'>ab9796</a>) for 5 minutes, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 3min

Supplier Data

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (AB197071)

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID : 23115189 and 20064930).

All lanes:

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/10000 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag at 20 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y516A mutant) with Myc-tag at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 140 kDa

false

Exposure time: 10s

Supplier Data

Dot Blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (AB197071)

Dot blot analysis of TrkA (phospho Y496) TrkB (phospho Y516) and TrkC (phospho Y516) peptides labeled with ab197071 at 1/1000 dilution.

Lane 1 : TrkB (phospho Y516) phospho peptide;

Lane 2 : TrkB Non-phospho peptide;

Lane 3 : TrkA (phospho Y496) phospho peptide;

Lane 4 : TrkA Non-phospho peptide;

Lane 5 : TrkC (phospho Y516) phospho peptide;

Lane6 : TrkC Non-phospho peptide.

Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Note : ab197071 reacts with TrkB (phospho Y516)/ TrkA (phospho Y496)/ TrkC (phospho Y516) according to the dot blot data.

Carrier free

Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19140

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Mouse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TrkA TrkB and TrkC also known as NTRK1 NTRK2 and NTRK3 respectively are tropomyosin receptor kinases. These proteins are transmembrane receptors containing extracellular ligand-binding domains a single transmembrane helix and an intracellular kinase domain. TrkA has a mass of about 140 kDa TrkB around 145 kDa and TrkC approximately 143 kDa. Expression of these receptors occurs mainly in the nervous system but they are also present in other tissues like the heart and pancreas. Each receptor exhibits selectivity for different neurotrophins with TrkA binding to nerve growth factor (NGF) TrkB binding to brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4) and TrkC recognizing neurotrophin-3 (NT-3).

Biological function summary

These Trk receptors play essential roles in the development of the nervous system. Upon binding their specific ligands Trk receptors undergo dimerization and autophosphorylation on key tyrosine residues. This phosphorylation activates downstream signaling cascades that promote neuron survival differentiation and axonal growth. The receptors are not part of a larger complex but they interact with adaptor proteins that link them to intracellular signaling pathways. These interactions enhance cellular processes like synaptic plasticity and memory formation.

Pathways

TrkA TrkB and TrkC are central to the MAPK/ERK and PI3K/AKT pathways. Activation of these pathways leads to neuronal survival and growth mediated by proteins like Ras and PI3K. These pathways highlight the connection of Trk receptors with other important signaling proteins such as Shc and PLCγ. Through these pathways they also contribute to cellular responses that influence nerve cell communication and survival under stress conditions.

Mutations or dysregulation of Trk receptors implicate them in several neurodegenerative disorders and cancers. Neurodegenerative conditions like Alzheimer's disease show altered Trk signaling while certain cancers including neuroblastoma involve TrkA mutations or amplifications. TrkB dysregulation is associated with mood disorders and obesity also interacting with BDNF in these conditions. Pathologically the relationship with connected proteins such as BDNF in TrkB-related pathways appears important in understanding these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the NTRK2 gene functions as a receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4, and can also bind to NTF3/neurotrophin-3, although this interaction is less efficient. Upon binding with its ligands, the receptor undergoes homodimerization, autophosphorylation, and activation, enabling it to recruit, phosphorylate, and/or activate several downstream effectors like SHC1, FRS2, SH2B1, SH2B2, and PLCG1. These effectors regulate distinct but overlapping signaling cascades. Through SHC1, FRS2, SH2B1, and SH2B2, NTRK2 activates the GRB2-Ras-MAPK cascade, which regulates neuronal differentiation including neurite outgrowth, as well as the Ras-PI3 kinase-AKT1 signaling cascade, which is primarily involved in growth and survival. The PLCG1 pathway, which influences synaptic plasticity, plays a role in learning and memory by affecting both short-term synaptic function and long-term potentiation. Additionally, PLCG1 activation leads to NF-Kappa-B activation, promoting the transcription of genes related to cell survival, thereby suppressing anoikis. NTRK2 may also be involved in neurotrophin-dependent calcium signaling in glial cells and in mediating communication between neurons and glial cells. This supplementary information is collated from multiple sources and compiled automatically.

See full target information NTRK2 phospho Y516

Additional targets

,NTRK1 phospho Y496,NTRK3 phospho Y516,

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

BMC complementary medicine and therapies 25:348 PubMed41034986

2025

Changji'an formula alleviates visceral hypersensitivity of a post-inflammatory IBS-D mouse model via NGF/TrkA signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Ke,Siyu Huang,He Zhu,Qinglong Tan,Huaiguo Li,Dongwen Liu,Fanghao Zheng,Shuncong Zhang,Kaijun Lei,Hongmei Tang

NPJ precision oncology 9:137 PubMed40348911

2025

A novel TRKB-activating internal tandem duplication characterizes a new mechanism of receptor tyrosine kinase activation.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren M Brown,Gabor Tax,Pablo Acera Mateos,Antoine de Weck,Steve Foresto,Thomas Robertson,Fatimah Jalud,Pamela Ajuyah,Paulette Barahona,Jie Mao,M Emmy M Dolman,Marie Wong,Chelsea Mayoh,Mark J Cowley,Loretta M S Lau,Teresa Sadras,Paul G Ekert

Development (Cambridge, England) 152: PubMed39803985

2025

A cardiac transcriptional enhancer is repurposed during regeneration to activate an anti-proliferative program.

Applications

Unspecified application

Species

Unspecified reactive species

Anupama Rao,Andrew Russell,Jose Segura-Bermudez,Charles Franz,Rejenae Dockery,Anton Blatnik,Jacob Panten,Mateo Zevallos,Carson McNulty,Maciej Pietrzak,Joseph Aaron Goldman

Bioactive materials 35:242-258 PubMed38333615

2024

Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoping Wu,Yi Zhou,Xianglin Hou,Weidong Liu,Wen Yin,Lei Wang,Yudong Cao,Zhipeng Jiang,Youwei Guo,Quan Chen,Wen Xie,Ziqiang Wang,Ning Shi,Yujun Liu,Xiang Gao,Longlong Luo,Jianwu Dai,Caiping Ren,Xingjun Jiang

Molecules (Basel, Switzerland) 27: PubMed36235036

2022

JND4135, a New Type II TRK Inhibitor, Overcomes TRK xDFG and Other Mutation Resistance In Vitro and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Wang,Yang Zhou,Xia Tang,Xiuwen Yu,Yongjin Wang,Shingpan Chan,Xiaojuan Song,Zhengchao Tu,Zhimin Zhang,Xiaoyun Lu,Zhang Zhang,Ke Ding

Cancers 12: PubMed32825119

2020

Identification of Novel Fusion Genes in Bone and Soft Tissue Sarcoma and Their Implication in the Generation of a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuyo Teramura,Miwa Tanaka,Yukari Yamazaki,Kyoko Yamashita,Yutaka Takazawa,Keisuke Ae,Seiichi Matsumoto,Takayuki Nakayama,Takao Kaneko,Yoshiro Musha,Takuro Nakamura

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140]

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (AB197071)

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (AB197071)

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (AB197071)

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (AB197071)

Dot Blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (AB197071)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (6)

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise