AB322462

Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160]

20ul selling size
Recombinant
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Rabbit Recombinant Multiclonal TrkA phospho Y496 + Y674 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Synthetic peptide - Human samples.

View Alternative Names

MTC, TRK, TRKA, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, Trk-A, TRKB, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B, TRKC, NT-3 growth factor receptor, GP145-TrkC, Neurotrophic tyrosine kinase receptor type 3, TrkC tyrosine kinase, Trk-C

3 Images

Supplier Data

Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (AB322462)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The bands beneath the target band are likely to be degraded target fragments

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

HeLa treated with 1mM pervanadate for 30 minutes, then replaced with 0.5mM H2O2 for 30 minutes, whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated A431 (human epidermoid carcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg

Lane 4:

A431 reated with 100 uM pervanadate for 30 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 5:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 6:

HeLa treated with 1mM pervanadate for 30 minutes, then replaced with 0.5mM H2O2 for 30 minutes, whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 7:

Untreated A431 (human epidermoid carcinoma epithelial cell) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 8:

A431 reated with 100 uM pervanadate for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 92 kDa,124 kDa

false

Exposure time: 15s

Supplier Data

Dot Blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (AB322462)

Dot blot analysis of TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) using ab322462 at 1 : 1000 dilution (0.511 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : Human TrkA (phospho Y496) peptide
Lane2 : Non-phospho TrKA control peptide for Lane 1
Lane3 : Human TrkA (phospho Y674) peptide
Lane4 : Human TrkA (phospho Y675) peptide
Lane5 : Non-phospho TrKA control peptide for Lane 3/4
Lane6 : Human TrkB (phospho Y516) peptide
Lane7 : Non-phospho TrKB control peptide for Lane 6
Lane8 : Human TrkB (phospho Y706) peptide
Lane9 : Human TrkB (phospho Y707) peptide
Lane10 : Non-phospho TrKB control peptide for Lane 8/9
Lane11 : Human TrkC (phospho Y516) peptide
Lane12 : Non-phospho TrKC control peptide for Lane 11
Lane13 : Human TrkC (phospho Y709) peptide
Lane14 : Human TrkC (phospho Y710) peptide
Lane15 : Non-phospho TrKC control peptide for Lane 13/14

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462) at 1/1000 dilution

Lane 1:

Human TrkA (phospho Y496) peptide

Lane 2:

Non-phospho TrKA control peptide for Lane 1

Lane 3:

Human TrkA (phospho Y674) peptide

Lane 4:

Human TrkA (phospho Y675) peptide

Lane 5:

Non-phospho TrKA control peptide for Lane 3/4

Lane 6:

Human TrkB (phospho Y516) peptide

Lane 7:

Non-phospho TrKB control peptide for Lane 6

Lane 8:

Human TrkB (phospho Y706) peptide

Lane 9:

Human TrkB (phospho Y707) peptide

Lane 10:

Non-phospho TrKB control peptide for Lane 8/9

Lane 11:

Human TrkC (phospho Y516) peptide

Lane 12:

Non-phospho TrKC control peptide for Lane 11

Lane 13:

Human TrkC (phospho Y709) peptide

Lane 14:

Human TrkC (phospho Y710) peptide

Lane 15:

Non-phospho TrKC control peptide for Lane 13/14

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Supplier Data

Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (AB322462)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human TrkA expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human TrkB expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human TrkC expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 92 kDa,140 kDa,36 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1160

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed : 1281417, PubMed : 15488758, PubMed : 17196528, PubMed : 1849459, PubMed : 1850821, PubMed : 22649032, PubMed : 27445338, PubMed : 8325889). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed : 1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.. Isoform TrkA-III. Resistant to NGF, it constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.

See full target information NTRK1 phospho Y496 + Y674

Additional targets

NTRK2 phospho Y516 + Y706,NT-3 growth factor receptor phospho Y516 + Y709

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Product promise

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For full details, please see our Terms & Conditions

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