Rabbit Recombinant Multiclonal TrkA phospho Y496 + Y674 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Synthetic peptide - Human samples.
Images
![Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (AB322462), expandable thumbnail](https://content.abcam.com/products/images/trka-phospho-y496y674trkb-phospho-y516y706trkc-phospho-y516y709-antibody-rm1160-ab322462--western-blot-img439094.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (AB322462)")
![Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (AB322462), expandable thumbnail](https://content.abcam.com/products/images/trka-phospho-y496y674trkb-phospho-y516y706trkc-phospho-y516y709-antibody-rm1160-ab322462--western-blot-img439095.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (AB322462)")
![Dot Blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (AB322462), expandable thumbnail](https://content.abcam.com/products/images/trka-phospho-y496y674trkb-phospho-y516y706trkc-phospho-y516y709-antibody-rm1160-ab322462--dot-blot-img439224.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (AB322462)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Dot | |
|---|---|---|
| Human | Tested | Expected |
| Synthetic peptide - Human | Not recommended | Tested |
| Transfected cell lysate - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Target data
Function
Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1281417, PubMed:15488758, PubMed:17196528, PubMed:1849459, PubMed:1850821, PubMed:22649032, PubMed:27445338, PubMed:8325889). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. Isoform TrkA-III. Resistant to NGF, it constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
Additional Targets
NTRK2 phospho Y516 + Y706, NT-3 growth factor receptor phospho Y516 + Y709
Alternative names
MTC, TRK, TRKA, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, Trk-A
Rabbit Recombinant Multiclonal TrkA phospho Y496 + Y674 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Synthetic peptide - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- RM1160
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462), expandable thumbnail](https://content.abcam.com/products/images/trka-phospho-y496y674trkb-phospho-y516y706trkc-phospho-y516y709-antibody-rm1160-ab322462--western-blot-img439094.jpg "Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462)")
Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462)
TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) Western blot staining using rabbit Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The bands beneath the target band are likely to be degraded target fragments
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462) at 1/1000 dilution
Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg
Lane 2: HeLa treated with 1mM pervanadate for 30 minutes, then replaced with 0.5mM H2O2 for 30 minutes, whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated A431 (human epidermoid carcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg
Lane 4: A431 reated with 100 uM pervanadate for 30 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 5: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 6: HeLa treated with 1mM pervanadate for 30 minutes, then replaced with 0.5mM H2O2 for 30 minutes, whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 7: Untreated A431 (human epidermoid carcinoma epithelial cell) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 8: A431 reated with 100 uM pervanadate for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 92 kDa, 124 kDa
Exposure time: 15s
![Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462), expandable thumbnail](https://content.abcam.com/products/images/trka-phospho-y496y674trkb-phospho-y516y706trkc-phospho-y516y709-antibody-rm1160-ab322462--western-blot-img439095.jpg "Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462)")
Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human TrkA expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human TrkB expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human TrkC expression vector containing a His-tag, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 92 kDa, 140 kDa, 36 kDa
Exposure time: 15s
![Dot Blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462), expandable thumbnail](https://content.abcam.com/products/images/trka-phospho-y496y674trkb-phospho-y516y706trkc-phospho-y516y709-antibody-rm1160-ab322462--dot-blot-img439224.jpg "Dot Blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462)")
Dot Blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462)
Dot blot analysis of TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) using ab322462 at 1:1000 dilution (0.511 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: Human TrkA (phospho Y496) peptide
Lane2: Non-phospho TrKA control peptide for Lane 1
Lane3: Human TrkA (phospho Y674) peptide
Lane4: Human TrkA (phospho Y675) peptide
Lane5: Non-phospho TrKA control peptide for Lane 3/4
Lane6: Human TrkB (phospho Y516) peptide
Lane7: Non-phospho TrKB control peptide for Lane 6
Lane8: Human TrkB (phospho Y706) peptide
Lane9: Human TrkB (phospho Y707) peptide
Lane10: Non-phospho TrKB control peptide for Lane 8/9
Lane11: Human TrkC (phospho Y516) peptide
Lane12: Non-phospho TrKC control peptide for Lane 11
Lane13: Human TrkC (phospho Y709) peptide
Lane14: Human TrkC (phospho Y710) peptide
Lane15: Non-phospho TrKC control peptide for Lane 13/14Exposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Dot Blot - Anti-TrkA (phospho Y496+Y674)+TrkB (phospho Y516+Y706)+TrkC (phospho Y516+Y709) antibody [RM1160] (ab322462) at 1/1000 dilution
Lane 1: Human TrkA (phospho Y496) peptide
Lane 2: Non-phospho TrKA control peptide for Lane 1
Lane 3: Human TrkA (phospho Y674) peptide
Lane 4: Human TrkA (phospho Y675) peptide
Lane 5: Non-phospho TrKA control peptide for Lane 3/4
Lane 6: Human TrkB (phospho Y516) peptide
Lane 7: Non-phospho TrKB control peptide for Lane 6
Lane 8: Human TrkB (phospho Y706) peptide
Lane 9: Human TrkB (phospho Y707) peptide
Lane 10: Non-phospho TrKB control peptide for Lane 8/9
Lane 11: Human TrkC (phospho Y516) peptide
Lane 12: Non-phospho TrKC control peptide for Lane 11
Lane 13: Human TrkC (phospho Y709) peptide
Lane 14: Human TrkC (phospho Y710) peptide
Lane 15: Non-phospho TrKC control peptide for Lane 13/14
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
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