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Rabbit Recombinant Monoclonal TrkB phospho Y706 antibody. Suitable for Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

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Images

Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (AB229908), expandable thumbnail
  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (AB229908), expandable thumbnail
  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (AB229908), expandable thumbnail
  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (AB229908), expandable thumbnail
  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (AB229908), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBIHC-PICC/IFChIP-seq
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Not recommended
Not recommended
Not recommended
Rat
Expected
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2 (PubMed:15494731, PubMed:7574684). Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:15494731). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

Additional Targets

NTRK1 phospho Y674, NTRK3 phospho Y709

Alternative names

TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B

Recommended products

Rabbit Recombinant Monoclonal TrkB phospho Y706 antibody. Suitable for Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22298-67
Purification technique
Affinity purification Protein A
Specificity

The antibody shows low affinity in the natural tissues of mouse and rat

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TrkB also known as Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) is a cell surface receptor with a mass of approximately 145 kDa. It binds brain-derived neurotrophic factor (BDNF) and is widely expressed in the central nervous system particularly in areas like the hippocampus cortex and cerebellum. TrkB belongs to the family of receptor tyrosine kinases functioning by catalyzing the transfer of a phosphate group from ATP to specific tyrosine residues on substrate proteins in the receptor triggering downstream signaling cascades.

Biological function summary

TrkB plays essential roles in neuronal survival differentiation and synaptic plasticity. It acts as an important mediator for the effects of neurotrophins primarily BDNF on neurons. While TrkB itself is not part of a multi-protein complex it interacts closely with several adaptor proteins post-activation to propagate survival and proliferation signals within the neuron. These biochemical interactions greatly influence neurodevelopment and cognitive functions.

Pathways

TrkB is a pivotal player in both the MAPK/ERK and PI3K/AKT pathways. Upon BDNF binding TrkB undergoes autophosphorylation triggering these pathways which are critically involved in cell survival and growth. Other proteins such as SHC1 and PLCγ interact with TrkB in these pathways extending the range of cellular responses to neurotrophin signaling facilitating processes like neuronal plasticity and memory formation.

Associated diseases and disorders

TrkB has significant implications in neurological conditions like Alzheimer's disease and depression. In Alzheimer's disease alterations in TrkB signaling can result in impaired cognitive functions and neuronal loss where it interacts with proteins like amyloid-beta contributing to disease progression. In depression dysfunctional BDNF-TrkB signaling impacts mood regulation and resilience linking TrkB with alterations in serotonin receptor signaling emphasizing its role in mood disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908), expandable thumbnail

    Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908)

    TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) Western blot staining using rabbit Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Observed MW: 140 kDA

    All lanes: Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908) at 1/1000 dilution

    Lane 1: Human brain at 20 µg

    Lane 2: Human Hippocampus at 20 µg

    Lane 3: Human cerebellum at 20 µg

    Lane 4: Human spinal cord at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 92 kDa

    Exposure time: 5s

  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908), expandable thumbnail

    Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908)

    TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) Western blot staining using rabbit Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Observed MW: 140 kDA

    All lanes: Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908) at 1/1000 dilution

    Lane 1: Mouse brain lysates at 20 µg

    Lane 2: Mouse hippocampus at 20 µg

    Lane 3: Mouse P7 thalamus at 20 µg

    Lane 4: Mouse cerebellum at 20 µg

    Lane 5: Rat brain at 20 µg

    Lane 6: Rat hippocampus at 20 µg

    Lane 7: Rat P7 thalamus at 20 µg

    Lane 8: Rat cerebellum at 20 µg

    Lane 9: Mouse P5 cerebral cortex at 20 µg

    Lane 10: Mouse P3 retina at 20 µg

    Lane 11: Mouse P20 retina at 20 µg

    Lane 12: Rat spinal cord at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 92 kDa

    Exposure time: 180s

  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908), expandable thumbnail

    Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908)

    TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) Western blot staining using rabbit Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The phosphorylation sites are conserved between TrkA and TrkB: Tyr674/675 of TrkA corresponds to Tyr706/707 in TrkB. Therefore, the TrkB (phospho T705) antibody can detect the phosphorylation of TrkA T674/675 in PC-12 cells treated with NGF. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23951412).

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908) at 1/1000 dilution

    Lane 1: Untreated PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

    Lane 2: PC-12 treated with 100ng/ml NGF (Recombinant human NGF protein (Active) ab9796) for 5 minutes at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 92 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908), expandable thumbnail

    Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908)

    TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) Western blot staining using rabbit Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24959744). Besides, TrkB auto-phosphorylation was observed when TrkB was over-expressed in HEK293 cells, which has been reported in the literature (PMID: 25632160).

    Exposure time: 7 seconds

    All lanes: Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908) at 1/5000 dilution

    Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

    Lane 2: HEK-293T transfected with human TrkB (WT) expression vector containing a myc-His-tag®, whole cell lysate (Untreated membrane) at 10 µg

    Lane 3: HEK-293T transfected with human TrkB (WT) expression vector containing a myc-His-tag®, whole cell lysate (Phosphatase treated membrane) at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 92 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908), expandable thumbnail

    Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908)

    TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) Western blot staining using rabbit Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24959744). Besides, TrkB auto-phosphorylation was observed when TrkB was over-expressed in HEK293 cells, which has been reported in the literature (PMID: 25632160).

    Exposure time: 48 seconds

    All lanes: Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908) at 1/5000 dilution

    Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

    Lane 2: HEK-293T transfected with mouse TrkB (WT) expression vector containing a myc-His-tag®, starved for 6 hours, whole cell lysate at 10 µg

    Lane 3: HEK-293T transfected with mouse TrkB (WT) expression vector containing a myc-His-tag®, starved for 6 hours, then treated with 50ng/ml BDNF (Recombinant Human/Murine/Rat BDNF protein (Active) ab9794) for 10 minutes, whole cell lysate (Untreated membrane) at 10 µg

    Lane 4: HEK-293T transfected with mouse TrkB (WT) expression vector containing a myc-His-tag®, starved for 6 hours, then treated with 50ng/ml BDNF (Recombinant Human/Murine/Rat BDNF protein (Active) ab9794) for 10 minutes, whole cell lysate (Phosphatase treated membrane) at 10 µg

    Lane 5: HEK-293T transfected with mouse TrkB (mutant Y705A) expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 92 kDa

    Observed band size: 140 kDa

  • Dot Blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908), expandable thumbnail

    Dot Blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908)

    TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) Dot Blot staining using rabbit Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody

    Dot blot analysis using ab229908 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.

    Lane 1: TrkB (phospho T705) peptide (aa697-708)

    Lane 2: TrkB (phospho T705) peptide (aa702-712)

    Lane 3: TrkB non-phospho peptide (aa697-712)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908), expandable thumbnail

    Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908)

    TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) Western blot staining using rabbit Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control at 1/200000 dilution.

    All lanes: Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human TrkA expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human TrkB expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a human TrkC expression vector containing a His-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 95 kDa, 140 kDa

    Exposure time: 15s

  • Dot Blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908), expandable thumbnail

    Dot Blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908)

    TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) Dot Blot staining using rabbit Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Dot Blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908) at 1/1000 dilution

    Lane 1: Human TrkA (phospho Y674) peptide

    Lane 2: Human TrkA (phospho Y675) peptide

    Lane 3: Human TrkA (phospho Y674/675) peptide1

    Lane 4: Human TrkA (phospho Y674/675) peptide2

    Lane 5: Human TrkA control peptide for Lane 1-4

    Lane 6: Human TrkB (phospho Y706) peptide

    Lane 7: Human TrkB (phospho Y707) peptide

    Lane 8: Human TrkB (phospho Y706/707) peptide

    Lane 9: Human TrkB control peptide for Lane 6-8

    Lane 10: Human TrkC (phospho Y709) peptide

    Lane 11: Human TrkC (phospho Y710) peptide

    Lane 12: Human TrkC (phospho Y709/710) peptide

    Lane 13: Human TrkC control peptide for Lane 10-12

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908), expandable thumbnail

    Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908)

    TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) Western blot staining using rabbit Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody

    Blocking buffer and dilution concentration: 5% NFDM/TBST.

    Lanes 1 - 4: Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (ab229908) at 1/1000 dilution

    Lanes 1 - 4: Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] - BSA and Azide free (Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] - BSA and Azide free ab264070) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human TrkA expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human TrkB expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a human TrkC expression vector containing a His-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 140 kDa

    Exposure time: 15s

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