JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB247744

Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TrkA antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

MTC, TRK, TRKA, NTRK1, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, Trk-A, TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)

This data was developed using ab109010, the same antibody clone in a different buffer formulation.

ab109010 showing negative staining in Normal human kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)

This data was developed using ab109010, the same antibody clone in a different buffer formulation.

ab109010 showing negative staining in Normal human colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)

This data was developed using ab109010, the same antibody clone in a different buffer formulation.

ab109010 at 1/250 dilution staining TrkA + TrkB in Human brain by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)

This data was developed using ab109010, the same antibody clone in a different buffer formulation.

ab109010 showing negative staining in Normal human liver tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)

This data was developed using ab109010, the same antibody clone in a different buffer formulation.

ab109010 showing negative staining in Normal human tonsil tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)
  • WB

Unknown

Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)

This data was developed using ab109010, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] (<a href='/en-us/products/primary-antibodies/trka-trkb-antibody-epr11042-ab109010'>ab109010</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Predicted band size: 42 kDa,69 kDa,79 kDa

Observed band size: 42 kDa

false

Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)
  • WB

Lab

Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] - BSA and Azide free (AB247744)

This data was developed using ab109010, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] (<a href='/en-us/products/primary-antibodies/trka-trkb-antibody-epr11042-ab109010'>ab109010</a>) at 1.118 µg/mL

Lanes 1, 3 and 5:

Empty vector overexpression 293T cell lysates at 20 µg

Lane 2:

His-Human TrkC overexpression 293T cell lysates at 20 µg

Lane 4:

His-Human TrkA overexpression 293T cell lysates at 20 µg

Lane 6:

His-Human TrkB overexpression 293T cell lysates at 20 µg

Lane 7:

Human brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Observed band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1104(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB304939

Human TrkA ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human TrkA ELISA Kit (AB304939)

  • 0
  • 0
Primary Antibodies

AB108319

Anti-BDNF antibody [EPR1292]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-BDNF antibody [EPR1292] (AB108319)

  • 4
  • 9
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB76291

Anti-Pan Trk antibody [EP1058Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EP1058Y] (AB76291)

  • 4
  • 11
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

ab247744 is the carrier-free version of ab109010.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TrkA also known as neurotrophic tyrosine receptor kinase 1 (NTRK1) and TrkB known as neurotrophic tyrosine receptor kinase 2 (NTRK2) are part of the tropomyosin receptor kinase family. TrkA has a mass of approximately 140 kDa while TrkB weighs around 145 kDa. These proteins express mainly in the nervous system including the brain and peripheral tissues. TrkA binds primarily to nerve growth factor (NGF) while TrkB interacts with brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT-4/5). They are important for neuronal development and function.
Biological function summary

TrkA and TrkB proteins promote survival differentiation and maintenance of specific neuronal populations. TrkA is involved in neuronal survival by dimerizing upon ligand binding activating downstream signaling pathways. This activity ensures proper neuronal function and structure. TrkB in addition to supporting neuronal differentiation also influences synaptic plasticity. Both act as receptors in the neurotrophin signaling pathway forming complexes with other intracellular proteins. These actions make TrkA and TrkB fundamental for proper nervous system development and response to damage.

Pathways

TrkA and TrkB are central to the MAPK/ERK and PI3K/Akt signaling pathways. The MAPK/ERK pathway influences cell growth and differentiation where TrkA activates Ras proteins leading to phosphorylation cascades. Meanwhile in the PI3K/Akt pathway activation of TrkB affects cell survival and apoptosis often involving interactions with proteins like Shc and PLCγ. These pathways are significant for neuronal cell fate decisions highlighting TrkA and TrkB’s interaction with other pathway components for maintaining cellular homeostasis.

TrkA and TrkB are linked to neurodegenerative diseases and cancers. Altered TrkA function or expression is associated with Alzheimer’s disease where downstream effects involve dysregulation of p75NTR a related protein. TrkB variations have been connected to psychiatric disorders such as depression. In some cancers increased expression or mutations in the TrkA or TrkB genes lead to tumorigenesis highlighting their importance in disease pathology. Understanding these connections aids in developing targeted therapies for these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed : 1281417, PubMed : 15488758, PubMed : 17196528, PubMed : 1849459, PubMed : 1850821, PubMed : 22649032, PubMed : 27445338, PubMed : 8325889). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed : 1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.. Isoform TrkA-III. Resistant to NGF, it constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
See full target information NTRK1

Additional targets

NTRK2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com