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Rabbit Recombinant Monoclonal TrkA phospho Y674 + Y675 antibody. Suitable for IHC-P, Dot and reacts with Transfected cell line - Human, Human, Synthetic peptide - Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] (AB316756), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] (AB316756), expandable thumbnail
  • Dot Blot - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] (AB316756), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PDotIPICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Mouse, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Mouse, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Mouse, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Target data

Function

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1281417, PubMed:15488758, PubMed:17196528, PubMed:1849459, PubMed:1850821, PubMed:22649032, PubMed:27445338, PubMed:8325889). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. Isoform TrkA-III. Resistant to NGF, it constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.

Additional Targets

NTRK2 phospho Y706 + Y707, NTRK3 phospho Y709 + Y710

Alternative names

MTC, TRK, TRKA, NTRK1, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, Trk-A

Recommended products

Rabbit Recombinant Monoclonal TrkA phospho Y674 + Y675 antibody. Suitable for IHC-P, Dot and reacts with Transfected cell line - Human, Human, Synthetic peptide - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28220-108
Purification technique
Affinity purification Protein A
Specificity

We have observed non-specific nuclear staining on some normal tissues, such as skin, colon and tonsil.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TrkA TrkB and TrkC also known as NTRK1 NTRK2 and NTRK3 respectively are tropomyosin receptor kinases. These proteins are transmembrane receptors containing extracellular ligand-binding domains a single transmembrane helix and an intracellular kinase domain. TrkA has a mass of about 140 kDa TrkB around 145 kDa and TrkC approximately 143 kDa. Expression of these receptors occurs mainly in the nervous system but they are also present in other tissues like the heart and pancreas. Each receptor exhibits selectivity for different neurotrophins with TrkA binding to nerve growth factor (NGF) TrkB binding to brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4) and TrkC recognizing neurotrophin-3 (NT-3).

Biological function summary

These Trk receptors play essential roles in the development of the nervous system. Upon binding their specific ligands Trk receptors undergo dimerization and autophosphorylation on key tyrosine residues. This phosphorylation activates downstream signaling cascades that promote neuron survival differentiation and axonal growth. The receptors are not part of a larger complex but they interact with adaptor proteins that link them to intracellular signaling pathways. These interactions enhance cellular processes like synaptic plasticity and memory formation.

Pathways

TrkA TrkB and TrkC are central to the MAPK/ERK and PI3K/AKT pathways. Activation of these pathways leads to neuronal survival and growth mediated by proteins like Ras and PI3K. These pathways highlight the connection of Trk receptors with other important signaling proteins such as Shc and PLCγ. Through these pathways they also contribute to cellular responses that influence nerve cell communication and survival under stress conditions.

Associated diseases and disorders

Mutations or dysregulation of Trk receptors implicate them in several neurodegenerative disorders and cancers. Neurodegenerative conditions like Alzheimer's disease show altered Trk signaling while certain cancers including neuroblastoma involve TrkA mutations or amplifications. TrkB dysregulation is associated with mood disorders and obesity also interacting with BDNF in these conditions. Pathologically the relationship with connected proteins such as BDNF in TrkB-related pathways appears important in understanding these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] (ab316756), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] (ab316756)

    Immunohistochemical analysis of paraffin-embedded HEK-293T (human embryonic kidney epithelial cell) transfected with (A) human TrkC expression vector containing a myc-His-tag; (B) human TrkA expression vector containing a myc-His-tag; (C) human TrkB expression vector containing a myc-His-tag; (D) human TrkA (Y674A and Y675A mutation) expression vector containing a myc-His-tag.

    A-D: whole cell pellet labelling with ab316756 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with ab316756 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

    Positive staining on HEK-293T overexpressing (A) TrkC, (B) TrkA, and (C) TrkB. No staining on HEK-293T overexpressing (D) TrkA (Y674A and Y675A mutation).

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] (ab316756), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] (ab316756)

    Immunohistochemical analysis of paraffin-embedded (A) KM-12 (human colon epithelial cell) untreated; (B) KM-12 treated with LOXO-101(10nM 2h); (C) KM-12 treated with alkaline phosphatase (after fixation and paraffin embedding).

    A-C: whole cell pellet labeling with ab316756 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with ab316756 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

    KM-12 express the constitutively active TPM3-NTRK1 fusion. Positive staining on (A) untreated KM-12. Reduced staining after (B) LOXO-101(10nM 2h) and (C) alkaline phosphatase treatment.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Dot Blot - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] (ab316756), expandable thumbnail

    Dot Blot - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] (ab316756)

    Dot blot analysis of TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) using ab316756 at 1:1000 (0.525 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    The antibody recognizes TrKA diphosphorylated at Y674 and Y675.

    All lanes: Dot Blot - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] (ab316756) at 1/1000 dilution

    Lane 1: TrkA (phospho Y674+Y675) peptide a with 5% NFDM/TBST

    Lane 2: TrkA (phospho Y674+Y675) peptide b with 5% NFDM/TBST

    Lane 3: TrkA (phospho Y674) peptide with 5% NFDM/TBST

    Lane 4: TrkA (phospho Y675) peptide with 5% NFDM/TBST

    Lane 5: TrkA non-phospho peptide with 5% NFDM/TBST

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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