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Rabbit Recombinant Monoclonal TrkA phospho Y674 + Y675 antibody. Carrier free. Suitable for IHC-P, Dot and reacts with Transfected cell line - Human, Human, Synthetic peptide - Human samples.

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Images

Dot Blot - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] - BSA and Azide free (AB316757), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] - BSA and Azide free (AB316757), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] - BSA and Azide free (AB316757), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PDotIPICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Mouse, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Mouse, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Mouse, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Target data

Function

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1281417, PubMed:15488758, PubMed:17196528, PubMed:1849459, PubMed:1850821, PubMed:22649032, PubMed:27445338, PubMed:8325889). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. Isoform TrkA-III. Resistant to NGF, it constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.

Additional Targets

NTRK2 phospho Y706 + Y707, NTRK3 phospho Y709 + Y710

Alternative names

MTC, TRK, TRKA, NTRK1, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, Trk-A

Recommended products

Rabbit Recombinant Monoclonal TrkA phospho Y674 + Y675 antibody. Carrier free. Suitable for IHC-P, Dot and reacts with Transfected cell line - Human, Human, Synthetic peptide - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28220-108
Purification technique
Affinity purification Protein A
Specificity

We have observed non-specific nuclear staining on some normal tissues, such as skin, colon and tonsil.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab316757 is the carrirer-free version of Anti-TrkA(pY674/Y675)+TrkB(pY706/Y707)+TrkC(pY709/Y710) antibody [EPR28220-108] ab316756.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TrkA TrkB and TrkC also known as NTRK1 NTRK2 and NTRK3 respectively are tropomyosin receptor kinases. These proteins are transmembrane receptors containing extracellular ligand-binding domains a single transmembrane helix and an intracellular kinase domain. TrkA has a mass of about 140 kDa TrkB around 145 kDa and TrkC approximately 143 kDa. Expression of these receptors occurs mainly in the nervous system but they are also present in other tissues like the heart and pancreas. Each receptor exhibits selectivity for different neurotrophins with TrkA binding to nerve growth factor (NGF) TrkB binding to brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4) and TrkC recognizing neurotrophin-3 (NT-3).

Biological function summary

These Trk receptors play essential roles in the development of the nervous system. Upon binding their specific ligands Trk receptors undergo dimerization and autophosphorylation on key tyrosine residues. This phosphorylation activates downstream signaling cascades that promote neuron survival differentiation and axonal growth. The receptors are not part of a larger complex but they interact with adaptor proteins that link them to intracellular signaling pathways. These interactions enhance cellular processes like synaptic plasticity and memory formation.

Pathways

TrkA TrkB and TrkC are central to the MAPK/ERK and PI3K/AKT pathways. Activation of these pathways leads to neuronal survival and growth mediated by proteins like Ras and PI3K. These pathways highlight the connection of Trk receptors with other important signaling proteins such as Shc and PLCγ. Through these pathways they also contribute to cellular responses that influence nerve cell communication and survival under stress conditions.

Associated diseases and disorders

Mutations or dysregulation of Trk receptors implicate them in several neurodegenerative disorders and cancers. Neurodegenerative conditions like Alzheimer's disease show altered Trk signaling while certain cancers including neuroblastoma involve TrkA mutations or amplifications. TrkB dysregulation is associated with mood disorders and obesity also interacting with BDNF in these conditions. Pathologically the relationship with connected proteins such as BDNF in TrkB-related pathways appears important in understanding these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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