AB251238

Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TrkA phospho Y496 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human, Rat samples. Cited in 1 publication.

View Alternative Names

MTC, TRK, TRKA, NTRK1, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, Trk-A, TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B, TRKC, NTRK3, NT-3 growth factor receptor, GP145-TrkC, Neurotrophic tyrosine kinase receptor type 3, TrkC tyrosine kinase, Trk-C

4 Images

Supplier Data

Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (AB251238)

This data was developed using ab197071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers (PMID : 23115189 and 20064930).

All lanes:

Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (<a href='/en-us/products/primary-antibodies/trka-phospho-y496-trkb-phospho-y516-trkc-phospho-y516-antibody-epr19140-ab197071'>ab197071</a>) at 1/2000 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes at 20 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 140 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (AB251238)

This data was developed using ab197071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Lane 1:

Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 2:

PC-12 treated with 100 ng/mL NGF (<a href='/en-us/products/proteins-peptides/recombinant-human-ngf-protein-active-ab9796'>ab9796</a>) for 5 minutes, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 140 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (AB251238)

This data was developed using ab197071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers (PMID : 23115189 and 20064930).

All lanes:

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag at 20 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y516A mutant) with Myc-tag at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 140 kDa

false

Exposure time: 10s

Supplier Data

Dot Blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (AB251238)

This data was developed using ab197071, the same antibody clone in a different buffer formulation.

Dot blot analysis of TrkA (phospho Y496), TrkB (phospho Y516), and TrkC (phospho Y516) peptides labeled with ab197071 at 1/1000 dilution.

Lane 1 : TrkB (phospho Y516) phospho peptide;
Lane 2 : TrkB Non-phospho peptide;
Lane 3 : TrkA (phospho Y496) phospho peptide;

Lane 4 : TrkA Non-phospho peptide;

Lane 5 : TrkC (phospho Y516) phospho peptide;

Lane6 : TrkC Non-phospho peptide.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Note : ab197071 reacts with TrkB (phospho Y516)/ TrkA (phospho Y496)/ TrkC (phospho Y516) according to the dot blot data.

Unconjugated

Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19140

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251238 is the carrier-free version of ab197071.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TrkA TrkB and TrkC also known as Ntrk1 Ntrk2 and Ntrk3 respectively are receptor tyrosine kinases expressed mainly in neurons of the central and peripheral nervous systems. TrkA has a mass of about 140 kDa TrkB about 145 kDa and TrkC around 140 kDa. These receptors bind to neurotrophins: TrkA interacts with nerve growth factor (NGF) TrkB with brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4) and TrkC with neurotrophin-3 (NT-3). Their primary role is to mediate neurotrophin signaling resulting in activation of downstream signaling cascades that support neuronal survival development and function.

Biological function summary

TrkA TrkB and TrkC play critical roles in nervous system development and maintenance. These receptors upon binding with their respective ligands form dimers which leads to autophosphorylation on specific tyrosine residues. This activation recruits adaptor proteins and through a series of interactions initiates multiple signaling pathways. Among these are the MAPK PI3K and PLCγ pathways which further propagate signals that regulate cell differentiation growth and survival demonstrating their importance in neurobiology.

Pathways

These Trk receptors engage in important signaling pathways that include the MAPK/ERK pathway and the PI3K/Akt pathway. These pathways are major components of cellular functions like proliferation differentiation and survival. During activation Trk receptors can interact with proteins like SHC and GRB2 within these pathways. Their signaling cascades influence neuronal plasticity and repair indicating their vital involvement in cellular responses to environmental stimuli.

TrkA TrkB and TrkC serve important roles in conditions like neurodegenerative diseases and certain cancers. For instance altered Trk receptor signaling has been associated with Alzheimer's disease where BDNF signaling via TrkB is often impaired. Similarly mutations or dysfunctional signaling of these receptors are implicated in cancers such as neuroblastoma where TrkA can act as an oncogene. These disorders highlight the relationship of these receptors to other proteins like tau in Alzheimer's disease and p75NTR in neuronal growth and cancerous transformations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed : 1281417, PubMed : 15488758, PubMed : 17196528, PubMed : 1849459, PubMed : 1850821, PubMed : 22649032, PubMed : 27445338, PubMed : 8325889). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed : 1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.. Isoform TrkA-III. Resistant to NGF, it constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.

See full target information NTRK1 phospho Y496

Additional targets

NTRK3 phospho Y516,NTRK2 phospho Y516

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:3361-3370 PubMed33682317

2021

TNFAIP8 regulates gastric cancer growth via mTOR-Akt-ULK1 pathway and autophagy signals.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Chen,Jianguo Zhang,Chenyang Dong,Dongsheng Li,Yuehan Yin,Wenhai Yu,Yuezhi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free

Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (AB251238)

Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (AB251238)

Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (AB251238)

Dot Blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (AB251238)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise