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AB134155

Anti-TrkB antibody [EPR1294]

5

(1 Review)

|

(12 Publications)

Rabbit Recombinant Monoclonal TrkB antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 12 publications.

View Alternative Names

TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (AB134155)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (AB134155)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelled with ab134155 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (AB134155)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (AB134155)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab134155 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (AB134155)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (AB134155)

Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134155 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (AB134155)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (AB134155)

Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab134155 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-TrkB antibody [EPR1294] (AB134155)
  • WB

Unknown

Western blot - Anti-TrkB antibody [EPR1294] (AB134155)

All lanes:

Western blot - Anti-TrkB antibody [EPR1294] (ab134155) at 1/1000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

Human hippocampus tissue lysate at 10 µg

Lane 3:

Human cerebellum tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1294

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Mouse, Rat: Internal testing data in Western Blot indicate that this antibody is not suitable for these species.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TrkB also known as Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) is a cell surface receptor with a mass of approximately 145 kDa. It binds brain-derived neurotrophic factor (BDNF) and is widely expressed in the central nervous system particularly in areas like the hippocampus cortex and cerebellum. TrkB belongs to the family of receptor tyrosine kinases functioning by catalyzing the transfer of a phosphate group from ATP to specific tyrosine residues on substrate proteins in the receptor triggering downstream signaling cascades.
Biological function summary

TrkB plays essential roles in neuronal survival differentiation and synaptic plasticity. It acts as an important mediator for the effects of neurotrophins primarily BDNF on neurons. While TrkB itself is not part of a multi-protein complex it interacts closely with several adaptor proteins post-activation to propagate survival and proliferation signals within the neuron. These biochemical interactions greatly influence neurodevelopment and cognitive functions.

Pathways

TrkB is a pivotal player in both the MAPK/ERK and PI3K/AKT pathways. Upon BDNF binding TrkB undergoes autophosphorylation triggering these pathways which are critically involved in cell survival and growth. Other proteins such as SHC1 and PLCγ interact with TrkB in these pathways extending the range of cellular responses to neurotrophin signaling facilitating processes like neuronal plasticity and memory formation.

TrkB has significant implications in neurological conditions like Alzheimer's disease and depression. In Alzheimer's disease alterations in TrkB signaling can result in impaired cognitive functions and neuronal loss where it interacts with proteins like amyloid-beta contributing to disease progression. In depression dysfunctional BDNF-TrkB signaling impacts mood regulation and resilience linking TrkB with alterations in serotonin receptor signaling emphasizing its role in mood disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2 (PubMed : 15494731, PubMed : 7574684). Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed : 15494731). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
See full target information NTRK2
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:292 PubMed40736675

2025

Proteome of oocyte spindle identifies Ccdc69 regulates spindle assembly like "band-tightening spell".

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ni Guo,Liu Zhu,Tie-Gang Meng,Yi-Na Zhang,Si-Min Sun,Xue-Mei Yang,Bing-Wang Zhao,Yi-Ke Lu,Yuan-Hong Xu,Wei Yue,Zhiming Han,Catherine C L Wong,Zhen-Bo Wang

Frontiers in psychiatry 16:1586613 PubMed40534913

2025

Correlation study of BDNF/TrkB/CREB, violence, and cognitive function in first-episode drug-naive schizophrenia patients.

Applications

Unspecified application

Species

Unspecified reactive species

Tiankai Jiang,Zhipeng Li,Tao Yu,Xudong Zhou,Tiantian Jiang,Yuhang Liang,Chen Yu,Min Zhu,Wenyu Wu

Oncogene 44:1805-1819 PubMed40133476

2025

Characterizing resistant cellular states in nasopharyngeal carcinoma during EBV lytic induction.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlei Wang,Lei Yu,Xuemeng Zhou,Grace Tin-Yun Chung,Alyssa Ming-Ting Liu,Yuk-Yu Chan,Man Wu,Kin Yung Chau,Kwok-Wai Lo,Angela Ruohao Wu

European journal of medical research 30:12 PubMed39773303

2025

GATA1-mediated macrophage polarization via TrkB/cGMP-PKG signaling pathway to promote the development of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Wushan Li,Fei Hou,Di Cheng,Fengchun Gao,Jin Wang,Baoxia Cui

Frontiers in molecular biosciences 11:1469775 PubMed39351154

2024

The synergistic effects of anoikis-related genes and EMT-related genes in the prognostic prediction of Wilms tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Kexin Meng,Zerui Zhao,Yaqing Gao,Keliang Wu,Wei Liu,Xiaoqing Wang,Yi Zheng,Wei Zhao,Bei Wang

Aging 15:3021-3034 PubMed37100464

2023

lncRNA sponges and inhibits BDNF/ERK pathway to suppress glioma cell proliferation and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Runhui Wang,Jia Wang,Yuanyu Wang,Liang Yang

Cell reports 40:111293 PubMed36044854

2022

RBM45 is an mA-binding protein that affects neuronal differentiation and the splicing of a subset of mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Seung H Choi,Mathieu N Flamand,Bei Liu,Huanyu Zhu,Meghan Hu,Melanie Wang,Jonathon Sewell,Christopher L Holley,Hashim M Al-Hashimi,Kate D Meyer

Laboratory investigation; a journal of technical methods and pathology 102:602-612 PubMed35152275

2022

Silencing of LINC01963 enhances the chemosensitivity of prostate cancer cells to docetaxel by targeting the miR-216b-5p/TrkB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zengshu Xing,Sailian Li,Jiansheng Xing,Gang Yu,Guoren Wang,Zhenxiang Liu

Frontiers in immunology 12:606861 PubMed33643311

2021

Tissue-Specificity of Antibodies Raised Against TrkB and p75 Receptors; Implications for Platelets as Models of Neurodegenerative Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Fleury,Imane Boukhatem,Jessica Le Blanc,Mélanie Welman,Marie Lordkipanidzé

Nature biomedical engineering 5:41-52 PubMed32719513

2020

Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.

Applications

Unspecified application

Species

Unspecified reactive species

Mahmoud Labib,Zongjie Wang,Sharif U Ahmed,Reza M Mohamadi,Bill Duong,Brenda Green,Edward H Sargent,Shana O Kelley
View all publications
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