AB187041

Anti-TrkB antibody [EPR17805-146]

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(36 Publications)

Anti-TrkB antibody [EPR17805-146] (ab187041) is a rabbit monoclonal antibody detecting TrkB in Western Blot, IP, IHC-P, ICC/IF, ELISA. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

Trkb, BDNF/NT-3 growth factors receptor, GP145-TrkB/GP95-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Trk-B

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (AB187041)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (AB187041)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling TrkB with ab187041 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and membrane staining on mouse cerebral cortex is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (AB187041)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling TrkB with ab187041 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and membrane staining on rat cerebral cortex is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] (AB187041)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] (AB187041)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TrkB with ab187041 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 2 ug/ml dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (AB187041)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TrkB with ab187041 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control : no staining on mouse liver [PMID : 2555172].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] (AB187041)

TrkB was immunoprecipitated from 1 mg of mouse brain lysate with ab187041 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab187041 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse brain lysate 10μg (Input).

Lane 2 : ab187041 IP in mouse brain lysate.

Lane 3 : Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab187041 in mouse brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] (ab187041)

Predicted band size: 92 kDa

false

Indirect ELISA - Anti-TrkB antibody [EPR17805-146] (AB187041)

I-ELISA

Lab

Indirect ELISA - Anti-TrkB antibody [EPR17805-146] (AB187041)

ELISA analysis of Mouse TrkB (Ntrk2) recombinant protein at 1000 ng/ml with ab187041. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-TrkB antibody [EPR17805-146] (AB187041)

Blocking/Dilution buffer : 5% NFDM/TBST.

Mouse TrkB active protein contains aa1-429 with a C-terminal poly histidine-tag.

All lanes:

Western blot - Anti-TrkB antibody [EPR17805-146] (ab187041) at 1/5000 dilution

All lanes:

Mouse TrkB active protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 171 kDa,87 kDa,92 kDa

Observed band size: 171 kDa,75-85 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-TrkB antibody [EPR17805-146] (AB187041)

Blocking/Dilution buffer : 5% NFDM/TBST.

TrkB is abundantly expressed in the central and peripheral nervous system. The 140 kDa observed MW which is higher than the predicted one is due to the glycosylation modification.

90 kDa corresponds to the truncated form, as observed in the literature (PMID : 24860020).

All lanes:

Western blot - Anti-TrkB antibody [EPR17805-146] (ab187041) at 1/5000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 140 kDa,90 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-TrkB antibody [EPR17805-146] (AB187041)

All lanes:

Western blot - Anti-TrkB antibody [EPR17805-146] (ab187041) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Rat lung tissue lysate at 20 µg

Lane 8:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,90 kDa

false

Exposure time: 10s

Carrier free

Anti-TrkB antibody [EPR17805-146] - BSA and Azide free (Detector)
Carrier free

Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free
Carrier free

Anti-TrkB antibody [EPR17805-146] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17805-146

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IP, I-ELISA, WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Recombinant fragment - Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-TrkB antibody [EPR17805-146] (ab187041) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Mouse, Rat samples.

What is the molecular weight of TrkB?
Anti-TrkB [EPR17805-146] (ab187041) specifically detects a band for TrkB (UniProt: Q16620) at a molecular weight of 92kDa.

Trusted by the scientific community
Anti-TrkB [EPR17805-146] (ab187041) was first used in a scientific publication in 2016 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR17805-146] also available for your convenience: ab187041, Carrier free - ab223155, Carrier free - ab242821, Carrier free - ab271952

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TrkB also known as Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) is a cell surface receptor with a mass of approximately 145 kDa. It binds brain-derived neurotrophic factor (BDNF) and is widely expressed in the central nervous system particularly in areas like the hippocampus cortex and cerebellum. TrkB belongs to the family of receptor tyrosine kinases functioning by catalyzing the transfer of a phosphate group from ATP to specific tyrosine residues on substrate proteins in the receptor triggering downstream signaling cascades.

Biological function summary

TrkB plays essential roles in neuronal survival differentiation and synaptic plasticity. It acts as an important mediator for the effects of neurotrophins primarily BDNF on neurons. While TrkB itself is not part of a multi-protein complex it interacts closely with several adaptor proteins post-activation to propagate survival and proliferation signals within the neuron. These biochemical interactions greatly influence neurodevelopment and cognitive functions.

Pathways

TrkB is a pivotal player in both the MAPK/ERK and PI3K/AKT pathways. Upon BDNF binding TrkB undergoes autophosphorylation triggering these pathways which are critically involved in cell survival and growth. Other proteins such as SHC1 and PLCγ interact with TrkB in these pathways extending the range of cellular responses to neurotrophin signaling facilitating processes like neuronal plasticity and memory formation.

TrkB has significant implications in neurological conditions like Alzheimer's disease and depression. In Alzheimer's disease alterations in TrkB signaling can result in impaired cognitive functions and neuronal loss where it interacts with proteins like amyloid-beta contributing to disease progression. In depression dysfunctional BDNF-TrkB signaling impacts mood regulation and resilience linking TrkB with alterations in serotonin receptor signaling emphasizing its role in mood disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

See full target information Ntrk2

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113489 PubMed41019369

2025

Therapeutic potential of 5HT2CR in alleviating spasticity and pain via modulation of motor neurons and interneurons in spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Han Gong,Zuliyaer Talifu,Yu-Zhe Sun,Zhao-Ming Yue,Xiao-Xia Du,Liang-Jie Du

Biomolecules 15: PubMed40867632

2025

Apelin-13-Mediated Upregulation of METTL3 Ameliorates Alzheimer's Disease via Inhibiting Neuroinflammation Through m6A-Dependent Regulation of lncRNA BDNF-AS.

Applications

Unspecified application

Species

Unspecified reactive species

Li Han,Siwen Wei,Rong Wang,Yiran Liu,Yi Zhong,Juan Fu,Huaiqing Luo,Meihua Bao

Frontiers in pharmacology 16:1592187 PubMed40453656

2025

The regulation and mechanism of the cAMP-PKA pathway on PTSD-like behaviors exacerbated by alcohol exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Zhao,Wei Zhao,Ziqi Wang,Xiaofei Chen,Fangjiao Zong,Hanting Zhang

Frontiers in neuroscience 18:1509543 PubMed39844852

2025

oligosaccharides alleviate chronic unpredictable mild stress-induced depression through the BDNF/TrkB/CREB pathway and symptoms of sexual dysfunction in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjie He,Mengying Hu,Tingqiao Wang,Zeping Zuo,Hongkai Li,Zhiwei Zhao,Yunwen Hao,Xueling Dai,Jianfang Wang,Yaxuan Sun

iScience 27:110379 PubMed39156650

2024

CCR3 knockdown attenuates prolonged underwater operations-induced cognitive impairment via alleviating microglia-mediated neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Houyu Zhao,Kun Liang,Zeyuan Yu,Yukun Wen,Xuhua Yu,Jiayun Xin,Tingting Zhang,Xianpeng Zu,Yiqun Fang

Frontiers in neuroscience 18:1402056 PubMed38872946

2024

Rehmanniae Radix Preparata ameliorates behavioral deficits and hippocampal neurodevelopmental abnormalities in ADHD rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Ruxin Sun,Haixia Yuan,Jing Wang,Kanglin Zhu,Yu Xiong,Yabei Zheng,Xinqiang Ni,Min Huang

General physiology and biophysics 43:175-183 PubMed38477607

2024

miR-497-5p promoted neuronal injury in ischemic stroke by inhibiting the BDNF/TrkB/PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Gong,Xiaona He,Guiliang Li,Dayu Wang,Yonghua Yang,Yanping Shi,Wenbing Su,Yuanxian Wu

CNS neuroscience & therapeutics 30:e14486 PubMed37830170

2023

Dexmedetomidine alleviates cognitive impairment by promoting hippocampal neurogenesis via BDNF/TrkB/CREB signaling pathway in hypoxic-ischemic neonatal rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Chen,Andi Chen,Jianjie Wei,Yongxin Huang,Jianhui Deng,Pinzhong Chen,Yanlin Yan,Mingxue Lin,Lifei Chen,Jiuyun Zhang,Zhibin Huang,Xiaoqian Zeng,Cansheng Gong,Xiaochun Zheng

Stem cell research & therapy 14:263 PubMed37735415

2023

Human umbilical cord-derived mesenchymal stem cells ameliorate perioperative neurocognitive disorder by inhibiting inflammatory responses and activating BDNF/TrkB/CREB signaling pathway in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Penghui Wei,Min Jia,Xiangyi Kong,Wenyuan Lyu,Hao Feng,Xinyi Sun,Jianjun Li,Jian-Jun Yang

Naunyn-Schmiedeberg's archives of pharmacology 397:1701-1714 PubMed37712973

2023

Agomelatine improves memory and learning impairments in a rat model of LPS-induced neurotoxicity by modulating the ERK/SorLA/BDNF/TrkB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mahmoud Abdelaziz,Ahmed F Mohamed,Hala F Zaki,Sameh S Gad

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TrkB antibody [EPR17805-146]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (AB187041)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (AB187041)

Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] (AB187041)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (AB187041)

Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] (AB187041)

Indirect ELISA - Anti-TrkB antibody [EPR17805-146] (AB187041)

Western blot - Anti-TrkB antibody [EPR17805-146] (AB187041)

Western blot - Anti-TrkB antibody [EPR17805-146] (AB187041)

Western blot - Anti-TrkB antibody [EPR17805-146] (AB187041)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

Product promise