Rabbit Recombinant Monoclonal TrkB antibody. Carrier free. Suitable for I-ELISA, IP, WB, ICC/IF, IHC-P and reacts with Recombinant fragment - Mouse, Mouse, Rat samples.
Images
![Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--indirect-elisa-img21550.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155)")
![Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--immunocytochemistry-immunofluorescence-img14839.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155)")
![Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--western-blot-img83161.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155)")
![Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--western-blot-img145126.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17955.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | IP | WB | ICC/IF | IHC-P | Flow Cyt | |
|---|---|---|---|---|---|---|
| Mouse | Expected | Tested | Tested | Tested | Tested | Not recommended |
| Rat | Expected | Expected | Tested | Expected | Tested | Not recommended |
| Recombinant fragment - Mouse | Tested | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
1 product for Alternative Version
Target data
Function
Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
Alternative names
Trkb, BDNF/NT-3 growth factors receptor, GP145-TrkB/GP95-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Trk-B
Recommended products
Rabbit Recombinant Monoclonal TrkB antibody. Carrier free. Suitable for I-ELISA, IP, WB, ICC/IF, IHC-P and reacts with Recombinant fragment - Mouse, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17805-146
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab223155 is the carrier-free version of Anti-TrkB antibody [EPR17805-146] ab187041.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TrkB also known as Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) is a cell surface receptor with a mass of approximately 145 kDa. It binds brain-derived neurotrophic factor (BDNF) and is widely expressed in the central nervous system particularly in areas like the hippocampus cortex and cerebellum. TrkB belongs to the family of receptor tyrosine kinases functioning by catalyzing the transfer of a phosphate group from ATP to specific tyrosine residues on substrate proteins in the receptor triggering downstream signaling cascades.
- Biological function summary
TrkB plays essential roles in neuronal survival differentiation and synaptic plasticity. It acts as an important mediator for the effects of neurotrophins primarily BDNF on neurons. While TrkB itself is not part of a multi-protein complex it interacts closely with several adaptor proteins post-activation to propagate survival and proliferation signals within the neuron. These biochemical interactions greatly influence neurodevelopment and cognitive functions.
- Pathways
TrkB is a pivotal player in both the MAPK/ERK and PI3K/AKT pathways. Upon BDNF binding TrkB undergoes autophosphorylation triggering these pathways which are critically involved in cell survival and growth. Other proteins such as SHC1 and PLCγ interact with TrkB in these pathways extending the range of cellular responses to neurotrophin signaling facilitating processes like neuronal plasticity and memory formation.
- Associated diseases and disorders
TrkB has significant implications in neurological conditions like Alzheimer's disease and depression. In Alzheimer's disease alterations in TrkB signaling can result in impaired cognitive functions and neuronal loss where it interacts with proteins like amyloid-beta contributing to disease progression. In depression dysfunctional BDNF-TrkB signaling impacts mood regulation and resilience linking TrkB with alterations in serotonin receptor signaling emphasizing its role in mood disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--indirect-elisa-img21550.jpg "Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
This data was developed using ab223155, the same antibody clone in a different buffer formulation.
ELISA analysis of Mouse TrkB (Ntrk2) recombinant protein at 1000 ng/ml with Anti-TrkB antibody [EPR17805-146] ab187041. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--immunocytochemistry-immunofluorescence-img14839.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
This data was developed using Anti-TrkB antibody [EPR17805-146] ab187041, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TrkB with Anti-TrkB antibody [EPR17805-146] ab187041 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
![Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--western-blot-img83161.jpg "Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
This data was developed using Anti-TrkB antibody [EPR17805-146] ab187041, the same clone in a different buffer formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
Mouse TrkB active protein contains aa1-429 with a C-terminal poly histidine-tag.
All lanes: Western blot - Anti-TrkB antibody [EPR17805-146] (Anti-TrkB antibody [EPR17805-146] ab187041) at 1/5000 dilution
All lanes: Mouse TrkB active protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 171 kDa, 87 kDa, 92 kDa
Observed band size: 171 kDa, 75-85 kDa
Exposure time: 3s
![Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--western-blot-img145126.jpg "Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
This data was developed using Anti-TrkB antibody [EPR17805-146] ab187041, the same clone in a different buffer formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
TrkB is abundantly expressed in the central and peripheral nervous system. The 140 kDa observed MW which is higher than the predicted one is due to the glycosylation modification.
90 kDa corresponds to the truncated form, as observed in the literature (PMID:24860020).
All lanes: Western blot - Anti-TrkB antibody [EPR17805-146] (Anti-TrkB antibody [EPR17805-146] ab187041) at 1/5000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Rat brain lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa, 90 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17955.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling TrkB with Anti-TrkB antibody [EPR17805-146] ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic and membrane staining on mouse cerebral cortex is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TrkB antibody [EPR17805-146] ab187041).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--immunoprecipitation-img68758.jpg "Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
TrkB was immunoprecipitated from 1 mg of mouse brain lysate with Anti-TrkB antibody [EPR17805-146] ab187041 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using Anti-TrkB antibody [EPR17805-146] ab187041 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse brain lysate 10μg (Input).
Lane 2: Anti-TrkB antibody [EPR17805-146] ab187041 IP in mouse brain lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TrkB antibody [EPR17805-146] ab187041 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TrkB antibody [EPR17805-146] ab187041).
All lanes: Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] (Anti-TrkB antibody [EPR17805-146] ab187041)
Predicted band size: 92 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img53440.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling TrkB with Anti-TrkB antibody [EPR17805-146] ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic and membrane staining on rat cerebral cortex is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TrkB antibody [EPR17805-146] ab187041).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img13439.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TrkB with Anti-TrkB antibody [EPR17805-146] ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative control: no staining on mouse liver [PMID: 2555172].
Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TrkB antibody [EPR17805-146] ab187041).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--western-blot-img445986.jpg "Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
TrkB Western blot staining using rabbit Anti-TrkB antibody
This data was developed using Anti-TrkB antibody [EPR17805-146] ab187041, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TrkB antibody [EPR17805-146] (Anti-TrkB antibody [EPR17805-146] ab187041) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Lane 4: Mouse kidney tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat heart tissue lysate at 20 µg
Lane 7: Rat lung tissue lysate at 20 µg
Lane 8: Rat kidney tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 140 kDa, 90 kDa
Exposure time: 10s
![Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--immunoprecipitation-img68758.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img53440.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img13439.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
![Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155), expandable thumbnail](https://content.abcam.com/products/images/trkb-antibody-epr17805-146-low-endotoxin-azide-free-ab223155--western-blot-img445986.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
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