AB326623

Anti-TrkB (phospho Y817) antibody [SC0556]

Recombinant
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Rabbit Recombinant Monoclonal TrkB phospho Y817 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples.

View Alternative Names

TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB (phospho Y817) antibody [SC0556] (AB326623)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB (phospho Y817) antibody [SC0556] (AB326623)

Formalin-fixed, paraffin-embedded human kidney tissue stained for TrkB (phospho Y817) using antibody at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TrkB (phospho Y817) antibody [SC0556] (AB326623)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TrkB (phospho Y817) antibody [SC0556] (AB326623)

ICC/IF staining of TrkB (phospho Y817) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with antibody at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
The cells were incubated 30 minutes at room temperature with phalloidin tritc .
Nuclei were counterstained with DAPI.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB (phospho Y817) antibody [SC0556] (AB326623)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for TrkB (phospho Y817) using antibody at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB (phospho Y817) antibody [SC0556] (AB326623)

Formalin-fixed, paraffin-embedded mouse pancreas tissue stained for TrkB (phospho Y817) using antibody at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-TrkB (phospho Y817) antibody [SC0556] (AB326623)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : SH-SY5Y whole cell lysates
Lane 2 : Mouse brain tissue lysates
Lane 3 : Rat brain tissue lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 145 kDa
Observed band size : 140 kDa

Exposure time : 12 seconds

All lanes:

Western blot - Anti-TrkB (phospho Y817) antibody [SC0556] (ab326623) at 1/1000 dilution

Lane 1:

SH-SY5Y whole cell lysates at 20 µg

Lane 2:

Mouse brain tissue lysates at 20 µg

Lane 3:

Rat brain tissue lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 145 kDa

Observed band size: 140 kDa

false

Exposure time: 12s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SC0556

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2 (PubMed : 15494731, PubMed : 7574684). Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed : 15494731). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

See full target information NTRK2 phospho Y817

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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