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Rabbit Recombinant Monoclonal TrkC antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.

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Images

Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (AB313761), expandable thumbnail
  • Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (AB313761), expandable thumbnail
  • Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (AB313761), expandable thumbnail
  • Western blot - Anti-TrkC antibody [EPR22959-404] (AB313761), expandable thumbnail
  • Western blot - Anti-TrkC antibody [EPR22959-404] (AB313761), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PIHC-FrICC/IFFlow CytFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Mouse
Dilution info
1/30
Notes

-

Species
Rat
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation.

Alternative names

TRKC, NTRK3, NT-3 growth factor receptor, GP145-TrkC, Neurotrophic tyrosine kinase receptor type 3, TrkC tyrosine kinase, Trk-C

Recommended products

Rabbit Recombinant Monoclonal TrkC antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22959-404
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TrkC also known as neurotrophic receptor tyrosine kinase 3 (NTRK3) is a receptor protein encoded by the NTRK3 gene. It weighs approximately 85 kDa and is expressed in the central and peripheral nervous systems as well as in several non-neuronal tissues. TrkC is a member of the TRK family of tyrosine kinase receptors which includes TrkA and TrkB. As a receptor TrkC binds to neurotrophin-3 (NT-3) to initiate signal transduction processes.

Biological function summary

TrkC is critical for neuronal development and function. It is part of a signaling complex that mediates the effects of the neurotrophin-3 involved in the survival differentiation and maintenance of various neuron populations. TrkC activation leads to the phosphorylation of the receptor and downstream signaling molecules impacting cellular outcomes. It has important roles in nervous system development and in processes such as synaptic plasticity and memory formation.

Pathways

TrkC participates in neurotrophin signaling and MAPK pathways. These pathways regulate important cellular activities like growth survival and differentiation. In the context of neurotrophin signaling related proteins such as TrkA and TrkB share similar pathway components influencing similar biological processes. TrkC plays a pivotal role in modulating pathway-related cellular responses and integrating signals from NT-3 to execute proper neuronal functioning.

Associated diseases and disorders

TrkC links to various neurological conditions including neurodevelopmental and mood disorders. Dysregulation or mutations in TrkC can lead to diseases such as medulloblastoma and congenital insensitivity to pain with anhidrosis (CIPA). The connection to NT-3 is significant in these conditions as disrupted TrkC/NT-3 signaling affects neuronal survival and development contributing to disease pathogenesis. The targeting of TrkC-related pathways holds potential for therapeutic interventions targeting these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761), expandable thumbnail

    Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761)

    TrkC was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab313761 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313761 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313761 in mouse brain tissue lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 6 seconds

    All lanes: Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/30 dilution

    All lanes: Mouse brain tissue lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 6s

  • Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761), expandable thumbnail

    Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761)

    TrkC was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab313761 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313761 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: Rat brain tissue lysate
    Lane 2: Rat brain tissue lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313761 in rat brain tissue lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 6 seconds

    All lanes: Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/30 dilution

    All lanes: Rat brain tissue lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 6s

  • Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761), expandable thumbnail

    Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761)

    TrkC was immunoprecipitated from 0.35 mg Human hypothalamus tissue lysate with ab313761 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313761 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: Human hypothalamus tissue lysate
    Lane 2: Human hypothalamus tissue lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313761 in human hypothalamus tissue lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 24 seconds

    All lanes: Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/30 dilution

    All lanes: Human hypothalamus tissue lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 24s

  • Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761), expandable thumbnail

    Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with human TrkA and human TrkB.
    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 6 seconds

    All lanes: Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human TrkC expression vector containing a His-tag whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human TrkA expression vector containing a His-tag whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a human TrkB expression vector containing a His-tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 145 kDa

    Exposure time: 6s

  • Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761), expandable thumbnail

    Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17828769).
    Exposure time: Lane 1: 6 seconds, lanes 2-3: 26 seconds.

    All lanes: Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/1000 dilution

    Lane 1: Human hypothalamus tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 145 kDa

  • Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761), expandable thumbnail

    Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: liver (PMID: 7823156).
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17828769).
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 15 seconds

    All lanes: Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/1000 dilution

    Lane 1: Mouse cerebellum tissue lysate at 20 µg

    Lane 2: Mouse hippocampus tissue lysate at 20 µg

    Lane 3: Mouse liver tissue lysate at 20 µg

    Lane 4: Rat cerebellum tissue lysate at 20 µg

    Lane 5: Rat hippocampus tissue lysate at 20 µg

    Lane 6: Rat liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 100 kDa, 145 kDa

    Exposure time: 15s

  • Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761), expandable thumbnail

    Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: liver (PMID: 7823156).
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17828769).
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 6 seconds

    All lanes: Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Human hippocampus tissue lysate at 20 µg

    Lane 3: human liver tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 100 kDa, 145 kDa

    Exposure time: 6s

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Product protocols

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