Anti-TrkC antibody [EPR22959-404]

• IP

Supplier Data

Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (AB313761)

TrkC was immunoprecipitated from 0.35 mg Human hypothalamus tissue lysate with ab313761 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313761 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Human hypothalamus tissue lysate Lane 2 : Human hypothalamus tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313761 in human hypothalamus tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/30 dilution

All lanes:

Human hypothalamus tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (AB313761)

TrkC was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab313761 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313761 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse brain tissue lysate Lane 2 : Mouse brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313761 in mouse brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 6 seconds

All lanes:

Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/30 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 6s

• IP

Supplier Data

Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (AB313761)

TrkC was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab313761 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313761 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Rat brain tissue lysate Lane 2 : Rat brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313761 in rat brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 6 seconds

All lanes:

Immunoprecipitation - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/30 dilution

All lanes:

Rat brain tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-TrkC antibody [EPR22959-404] (AB313761)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 17828769). Exposure time : Lane 1 : 6 seconds, lanes 2-3 : 26 seconds.

All lanes:

Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 145 kDa

false

• WB

Supplier Data

Western blot - Anti-TrkC antibody [EPR22959-404] (AB313761)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver (PMID : 7823156). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 17828769). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 6 seconds

All lanes:

Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human hippocampus tissue lysate at 20 µg

Lane 3:

human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 100 kDa,145 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-TrkC antibody [EPR22959-404] (AB313761)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver (PMID : 7823156). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 17828769). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Rat cerebellum tissue lysate at 20 µg

Lane 5:

Rat hippocampus tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa,145 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-TrkC antibody [EPR22959-404] (AB313761)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human TrkA and human TrkB. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 6 seconds

All lanes:

Western blot - Anti-TrkC antibody [EPR22959-404] (ab313761) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human TrkC expression vector containing a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human TrkA expression vector containing a His-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human TrkB expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 145 kDa

false

Exposure time: 6s