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AB283652

Anti-TRM1 antibody [EPR25054-72]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

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Rabbit Recombinant Monoclonal TRMT1 antibody. Suitable for WB and reacts with Rat, Human samples.

View Alternative Names

tRNA (guanine(26)-N(2))-dimethyltransferase, TRMT1

3 Images
Western blot - Anti-TRM1 antibody [EPR25054-72] (AB283652)
  • WB

Lab

Western blot - Anti-TRM1 antibody [EPR25054-72] (AB283652)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-TRM1 antibody [EPR25054-72] (ab283652) at 1/1000 dilution

All lanes:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 75 kDa

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Western blot - Anti-TRM1 antibody [EPR25054-72] (AB283652)
  • WB

Lab

Western blot - Anti-TRM1 antibody [EPR25054-72] (AB283652)

Blocking and diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

Lanes 1-3 : Merged signal (red and green). Green - ab283652 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa. ab283652 Anti-TRMT1 (TRM1) antibody [EPR25054-72] was shown to specifically react with TRMT1 (TRM1) in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266705 (knockout cell lysate ab258252) was used. Wild-type and TRMT1 (TRM1) knockout samples were subjected to SDS-PAGE. ab283652 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

The bands nearby 100 kDa could be non-specific bands.

All lanes:

Western blot - Anti-TRM1 antibody [EPR25054-72] (ab283652) at 1/1000 dilution

Lane 1:

Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

TRMT1 (TRM1) knockout HEK293T (<a href='/en-us/products/cell-lines/human-trmt1-trm1-knockout-hek-293t-cell-line-ab266705'>ab266705</a>) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TRMT1 (TRM1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-trmt1-trm1-knockout-hek-293t-cell-line-ab266705'>ab266705</a>)

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>), 1/10000 dilution

Predicted band size: 72 kDa

Observed band size: 75 kDa

false

Western blot - Anti-TRM1 antibody [EPR25054-72] (AB283652)
  • WB

Lab

Western blot - Anti-TRM1 antibody [EPR25054-72] (AB283652)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The bands nearby 100 kDa and 17 kDa could be non-specific bands.

Exposure time : 48 seconds

All lanes:

Western blot - Anti-TRM1 antibody [EPR25054-72] (ab283652) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

Hela transfected with TRM1 siRNA 1 whole cell lysate at 40 µg

Lane 3:

Hela transfected with TRM1 siRNA 2 whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 48 kDa

Observed band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25054-72

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRM1 also known as transfer RNA methyltransferase 1 is an enzyme responsible for methylating the N2 position of guanine in tRNA molecules. It has a molecular mass of approximately 49 kDa. TRM1 mostly resides in the mitochondria but can also be found in the cytoplasm. Its presence ensures proper methylation of tRNA influencing efficient and accurate protein translation.
Biological function summary

The methylation activity of TRM1 plays a role in maintaining the stability and function of tRNA molecules. As part of the cellular machinery TRM1 does not function alone; it often works together with other tRNA-modifying enzymes to ensure the fidelity of protein synthesis. This modification protects tRNA's structure facilitates proper folding and enhances interaction with ribosomal components.

Pathways

TRM1 functions significantly impact protein synthesis pathways. The enzyme is instrumental in the translation process by ensuring that tRNA molecules are correctly modified for protein assembly. TRM1 operates alongside other proteins such as TRM10 and TRMT5 within these modification pathways influencing the efficiency and accuracy of translation. It supports processes like tRNA maturation and ribosomal activity.

Alterations in TRM1 activity can relate to mitochondrial pathologies. Mutations or deficiencies in TRM1 can disrupt mitochondrial function leading to disorders such as mitochondrial myopathy. Research also connects TRM1 aberrations to neurodegenerative diseases where it may interact with proteins like PINK1 highlighting its role in neuroprotective responses. The efficiency of TRM1 in performing its tasks is vital as any dysfunction can result in profound cellular consequences affecting human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.
See full target information TRMT1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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