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AB283664

Anti-TRM1 antibody [EPR25054-72] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TRMT1 antibody. Carrier free. Suitable for WB and reacts with Rat, Human samples. Cited in 1 publication.

View Alternative Names

tRNA (guanine(26)-N(2))-dimethyltransferase, tRNA methyltransferase 1, hTRM1, TRMT1

2 Images
Western blot - Anti-TRM1 antibody [EPR25054-72] - BSA and Azide free (AB283664)
  • WB

Lab

Western blot - Anti-TRM1 antibody [EPR25054-72] - BSA and Azide free (AB283664)

This data was developed using ab283652, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The bands nearby 100 kDa and 17 kDa could be non-specific bands.

Exposure time : 48 seconds

All lanes:

Western blot - Anti-TRM1 antibody [EPR25054-72] (<a href='/en-us/products/primary-antibodies/trm1-antibody-epr25054-72-ab283652'>ab283652</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

Hela transfected with TRM1 siRNA 1 whole cell lysate at 40 µg

Lane 3:

Hela transfected with TRM1 siRNA 2 whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 48 kDa

Observed band size: 75 kDa

false

Western blot - Anti-TRM1 antibody [EPR25054-72] - BSA and Azide free (AB283664)
  • WB

Lab

Western blot - Anti-TRM1 antibody [EPR25054-72] - BSA and Azide free (AB283664)

This data was developed using ab283652, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-TRM1 antibody [EPR25054-72] (<a href='/en-us/products/primary-antibodies/trm1-antibody-epr25054-72-ab283652'>ab283652</a>) at 1/1000 dilution

All lanes:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25054-72

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab283664 is the carrier-free version of ab283652.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRM1 also known as transfer RNA methyltransferase 1 is an enzyme responsible for methylating the N2 position of guanine in tRNA molecules. It has a molecular mass of approximately 49 kDa. TRM1 mostly resides in the mitochondria but can also be found in the cytoplasm. Its presence ensures proper methylation of tRNA influencing efficient and accurate protein translation.
Biological function summary

The methylation activity of TRM1 plays a role in maintaining the stability and function of tRNA molecules. As part of the cellular machinery TRM1 does not function alone; it often works together with other tRNA-modifying enzymes to ensure the fidelity of protein synthesis. This modification protects tRNA's structure facilitates proper folding and enhances interaction with ribosomal components.

Pathways

TRM1 functions significantly impact protein synthesis pathways. The enzyme is instrumental in the translation process by ensuring that tRNA molecules are correctly modified for protein assembly. TRM1 operates alongside other proteins such as TRM10 and TRMT5 within these modification pathways influencing the efficiency and accuracy of translation. It supports processes like tRNA maturation and ribosomal activity.

Alterations in TRM1 activity can relate to mitochondrial pathologies. Mutations or deficiencies in TRM1 can disrupt mitochondrial function leading to disorders such as mitochondrial myopathy. Research also connects TRM1 aberrations to neurodegenerative diseases where it may interact with proteins like PINK1 highlighting its role in neuroprotective responses. The efficiency of TRM1 in performing its tasks is vital as any dysfunction can result in profound cellular consequences affecting human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dimethylates a single guanine residue at position 26 of most nuclear- and mitochondrial-encoded tRNAs using S-adenosyl-L-methionine as donor of the methyl groups (PubMed : 10982862, PubMed : 28784718, PubMed : 37204604, PubMed : 39786990). tRNA guanine(26)-dimethylation is required for redox homeostasis and ensure proper cellular proliferation and oxidative stress survival (PubMed : 28784718).
See full target information TRMT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 7:28040-58 PubMed27057631

2016

Age-related effects of X-ray irradiation on mouse hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Arianna Casciati,Katalin Dobos,Francesca Antonelli,Anett Benedek,Stefan J Kempf,Montserrat Bellés,Andrea Balogh,Mirella Tanori,Luis Heredia,Michael J Atkinson,Christine von Toerne,Omid Azimzadeh,Anna Saran,Geza Sáfrány,Mohammed A Benotmane,M Victoria Linares-Vidal,Soile Tapio,Katalin Lumniczky,Simonetta Pazzaglia
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com