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AB249806

Anti-TRNP1 antibody [EPR11958] - BSA and Azide free

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Rabbit Recombinant Monoclonal TRNP1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

C1orf225, TRNP, TRNP1, TMF-regulated nuclear protein 1

2 Images
Western blot - Anti-TRNP1 antibody [EPR11958] - BSA and Azide free (AB249806)
  • WB

Supplier Data

Western blot - Anti-TRNP1 antibody [EPR11958] - BSA and Azide free (AB249806)

This data was developed using ab174303, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TRNP1 antibody [EPR11958] (<a href='/en-us/products/primary-antibodies/trnp1-antibody-epr11958-ab174303'>ab174303</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 23 kDa

Observed band size: 36 kDa

false

Immunoprecipitation - Anti-TRNP1 antibody [EPR11958] - BSA and Azide free (AB249806)
  • IP

Supplier Data

Immunoprecipitation - Anti-TRNP1 antibody [EPR11958] - BSA and Azide free (AB249806)

This data was developed using ab174303, the same antibody clone in a different buffer formulation.

Immunoprecipitation. ab174303 at 1/1000 labeling TRNP1 immunoprecipitated from HeLa cell lysate using ab174303 at 1/10.

All lanes:

Immunoprecipitation - Anti-TRNP1 antibody [EPR11958] (<a href='/en-us/products/primary-antibodies/trnp1-antibody-epr11958-ab174303'>ab174303</a>)

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11958

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249806 is the carrier-free version of ab174303.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRNP1 also known as Ternary Complex Factor Small Nuclear Ribonucleic Protein 1 is a protein involved in DNA-binding activities. It has a mass of approximately 25 kDa. TRNP1 is expressed in various tissues most predominantly in the brain and neural tissues. It acts as a transcription factor binding to specific DNA sequences and influences the expression of genes involved in brain development and neural cell proliferation.
Biological function summary

TRNP1 regulates the proliferation and differentiation of neural progenitor cells. This protein does not appear to form part of a larger protein complex but it directly affects cellular processes that shape the growth and arrangement of the cerebral cortex during embryonic development. Its expression levels can control the cellular architecture influencing neural tube closure and cortical folding.

Pathways

TRNP1 plays a role in neural development pathways and interacts significantly with the Wnt signaling pathway. It regulates genes that are important for maintaining the balance between neural progenitor cell proliferation and differentiation. In this pathway TRNP1 may have an interaction with proteins like Wnt and β-catenin that control cellular growth decisions affecting brain size and function eventually.

Alterations in TRNP1 levels or function relate to disorders such as microcephaly and possibly certain neurodevelopmental dysplasias. It has been suggested that imbalances in TRNP1 activity can disrupt cortical development leading to abnormal brain development. While comprehensive studies are ongoing TRNP1 potentially interacts with proteins associated with these disorders marking its importance in neural developmental research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex. Regulates neural stem cells proliferation and the production of intermediate neural progenitors and basal radial glial cells affecting the process of cerebral cortex gyrification. May control the proliferation rate of cells by regulating their progression through key cell-cycle transition points (By similarity).
See full target information TRNP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com