AB224536

Anti-TRNT1 antibody

Name: Anti-TRNT1 antibody (ab224536) | Abcam
Brand: Abcam Limited
SKU: AB224536

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(2 Publications)

Rabbit Polyclonal TRNT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRNT1 aa 300-400.

View Alternative Names

CGI-47, TRNT1, mt CCA-adding enzyme, mt tRNA CCA-diphosphorylase, mt tRNA CCA-pyrophosphorylase, mt tRNA adenylyltransferase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRNT1 antibody (AB224536)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRNT1 antibody (AB224536)

Paraffin-embedded human stomach tissue stained for TRNT1 using ab224536 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TRNT1 antibody (AB224536)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRNT1 antibody (AB224536)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TRNT1 (green) using ab224536 at 4 μg/ml in ICC/IF.

Supplier Data

Western blot - Anti-TRNT1 antibody (AB224536)

All lanes:

Western blot - Anti-TRNT1 antibody (ab224536) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 50 kDa

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Supplier Data

Western blot - Anti-TRNT1 antibody (AB224536)

All lanes:

Western blot - Anti-TRNT1 antibody (ab224536) at 1/100 dilution

All lanes:

A549 (human lung carcinoma cell line) cell lysate

Predicted band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TRNT1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

Q96Q11

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRNT1 also known as tRNA nucleotidyl transferase 1 is a protein that attaches the CCA sequence to the 3' end of tRNA molecules. This addition is essential for transferring amino acids during protein synthesis. TRNT1 has a molecular mass of about 48 kDa. The protein is expressed in many tissues but is particularly abundant in metabolically active tissues like heart and liver where protein synthesis is high.

Biological function summary

The addition of the CCA tail is a necessary step at the end of tRNA maturation. Without this tRNA molecules cannot function in protein synthesis. TRNT1 does not work alone; it complements the function of other tRNA modifying enzymes adding the correct nucleotides to tRNA. TRNT1 frequently interacts with other molecules to ensure the processing is accurate and efficient.

Pathways

TRNA CCA addition by TRNT1 plays a role in RNA processing and protein synthesis pathways. These pathways are fundamental for cell function impacting how cells make proteins. TRNT1 interacts with other RNA-related proteins like tRNA synthetases which help link tRNA to specific amino acids. This interaction shows the connectedness of TRNT1 with proteins involved in translational machinery.

Improper function of TRNT1 associates with sideroblastic anemia with B-cell immunodeficiency periodic fevers and developmental delay (SIFD). In this context inadequate TRNT1 activity leads to defective tRNA maturation contributing to disease symptoms. TRNT1’s malfunction can also relate to other proteins in these diseases such as mutant forms of certain mitochondrial proteins exacerbating clinical features.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates (PubMed : 11504732, PubMed : 25193871, PubMed : 25640237, PubMed : 25652405, PubMed : 29454993, PubMed : 30959222, PubMed : 31011209, PubMed : 34023389). tRNA 3'-terminal CCA addition is required both for tRNA processing and repair (PubMed : 22076379, PubMed : 25640237). Promotes tRNA repair and recycling downstream of the ribosome-associated quality control (RQC) pathway by mediating addition of the tRNA 3'-terminal CCA following cleavage by ANKZF1 and repair by ELAC1 (PubMed : 31011209). Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs and tRNA-like transcripts (PubMed : 22076379, PubMed : 25640237). While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs beginning with GG are marked with CCACCA and rapidly degraded (PubMed : 22076379, PubMed : 25640237). The structural flexibility of RNA controls the choice between CCA versus CCACCA addition : following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA (PubMed : 25640237). This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle (PubMed : 25640237).. Isoform 2. Adds 2 C residues (CC-) to the 3' terminus of tRNA molecules instead of a complete CCA end as isoform 1 does (in vitro).

See full target information TRNT1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pediatrics 11:1160107 PubMed37215601

2023

Case report: Muscle involvement in a Chinese patient with -related disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Jie Wei,Yi-Dan Liu,Yan-Ling Yang,Yuan Wu,Jie-Yu Liu,Xing-Zhi Chang,Ying Hua,Yu-He Liu,Hui Xiong

eLife 9: PubMed33164744

2020

Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.

Applications

Unspecified application

Species

Unspecified reactive species

Alex J B Kreutzberger,Volker Kiessling,Catherine A Doyle,Noah Schenk,Clint M Upchurch,Margaret Elmer-Dixon,Amanda E Ward,Julia Preobraschenski,Syed S Hussein,Weronika Tomaka,Patrick Seelheim,Iman Kattan,Megan Harris,Binyong Liang,Anne K Kenworthy,Bimal N Desai,Norbert Leitinger,Arun Anantharam,J David Castle,Lukas K Tamm

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TRNT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRNT1 antibody (AB224536)

Immunocytochemistry/ Immunofluorescence - Anti-TRNT1 antibody (AB224536)

Western blot - Anti-TRNT1 antibody (AB224536)

Western blot - Anti-TRNT1 antibody (AB224536)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise