Rabbit Polyclonal TRNT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRNT1 aa 300-400.
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Publications
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- Frontiers in pediatrics 11:11601072023Case report: Muscle involvement in a Chinese patient with -related disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCui-Jie Wei,Yi-Dan Liu,Yan-Ling Yang,Yuan Wu,Jie-Yu Liu,Xing-Zhi Chang,Ying Hua,Yu-He Liu,Hui XiongPubMed 37215601
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TRNT1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q96Q11
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates (PubMed:11504732, PubMed:25193871, PubMed:25640237, PubMed:25652405, PubMed:29454993, PubMed:30959222, PubMed:31011209, PubMed:34023389). tRNA 3'-terminal CCA addition is required both for tRNA processing and repair (PubMed:22076379, PubMed:25640237). Promotes tRNA repair and recycling downstream of the ribosome-associated quality control (RQC) pathway by mediating addition of the tRNA 3'-terminal CCA following cleavage by ANKZF1 and repair by ELAC1 (PubMed:31011209). Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs and tRNA-like transcripts (PubMed:22076379, PubMed:25640237). While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs beginning with GG are marked with CCACCA and rapidly degraded (PubMed:22076379, PubMed:25640237). The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA (PubMed:25640237). This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle (PubMed:25640237). Isoform 2. Adds 2 C residues (CC-) to the 3' terminus of tRNA molecules instead of a complete CCA end as isoform 1 does (in vitro).
Alternative names
CGI-47, TRNT1, mt CCA-adding enzyme, mt tRNA CCA-diphosphorylase, mt tRNA CCA-pyrophosphorylase, mt tRNA adenylyltransferase
Recommended products
Rabbit Polyclonal TRNT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRNT1 aa 300-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TRNT1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q96Q11
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRNT1 also known as tRNA nucleotidyl transferase 1 is a protein that attaches the CCA sequence to the 3' end of tRNA molecules. This addition is essential for transferring amino acids during protein synthesis. TRNT1 has a molecular mass of about 48 kDa. The protein is expressed in many tissues but is particularly abundant in metabolically active tissues like heart and liver where protein synthesis is high.
- Biological function summary
The addition of the CCA tail is a necessary step at the end of tRNA maturation. Without this tRNA molecules cannot function in protein synthesis. TRNT1 does not work alone; it complements the function of other tRNA modifying enzymes adding the correct nucleotides to tRNA. TRNT1 frequently interacts with other molecules to ensure the processing is accurate and efficient.
- Pathways
TRNA CCA addition by TRNT1 plays a role in RNA processing and protein synthesis pathways. These pathways are fundamental for cell function impacting how cells make proteins. TRNT1 interacts with other RNA-related proteins like tRNA synthetases which help link tRNA to specific amino acids. This interaction shows the connectedness of TRNT1 with proteins involved in translational machinery.
- Associated diseases and disorders
Improper function of TRNT1 associates with sideroblastic anemia with B-cell immunodeficiency periodic fevers and developmental delay (SIFD). In this context inadequate TRNT1 activity leads to defective tRNA maturation contributing to disease symptoms. TRNT1’s malfunction can also relate to other proteins in these diseases such as mutant forms of certain mitochondrial proteins exacerbating clinical features.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-TRNT1 antibody (ab224536)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TRNT1 (green) using ab224536 at 4 μg/ml in ICC/IF.
Western blot - Anti-TRNT1 antibody (ab224536)
All lanes: Western blot - Anti-TRNT1 antibody (ab224536) at 1/100 dilution
All lanes: A549 (human lung carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRNT1 antibody (ab224536)
Paraffin-embedded human stomach tissue stained for TRNT1 using ab224536 at 1/200 dilution in immunohistochemical analysis.
Western blot - Anti-TRNT1 antibody (ab224536)
All lanes: Western blot - Anti-TRNT1 antibody (ab224536) at 1/100 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2: NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 50 kDa
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