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Rabbit Recombinant Monoclonal TROP2 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples.

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Images

Western blot - Anti-TROP2 antibody [SP293] - BSA and Azide free (AB272014), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (AB272014), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TROP2 antibody [SP293] - BSA and Azide free (AB272014), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (AB272014), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (AB272014), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Tested
Expected

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Boil tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. ;

Species

Mouse

Dilution info

-

Notes

Boil tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. ;

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

8 products for Alternative Version

Target data

Function

May function as a growth factor receptor.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TROP2 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

SP293

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab272014 is the carrier-free version of Anti-TROP2 antibody [SP293] - C-terminal ab227689.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TROP2 also known as TACSTD2 or tumor-associated calcium signal transducer 2 is a single-pass type I membrane glycoprotein. This protein is about 35 kDa in mass and primarily expresses in epithelial tissues. It acts as a transmembrane receptor that plays a role in signal transduction due to its domain structure binding and activation processes on the cell surface. TROP2 exhibits high expression in several types of tumors making it a potential target for therapeutic antibodies like anti-TROP2 anti trop2 or anti-TROP-2.

Biological function summary

TROP2 mediates calcium signaling and cell proliferation. It functions by being a part of a signaling complex on the cell membrane which affects multiple cellular activities such as cell growth motility and survival. Its role in maintaining the integrity of the epithelial cell layer also shows TROP2's significance in normal tissue homeostasis. The interaction of TROP2 with the extracellular matrix and other cell surface proteins support the structural integrity and communication between cells.

Pathways

TROP2 plays a central role in the PI3K/AKT and MAPK signaling pathways that regulate cell growth and survival. Within these pathways TROP2 works in concert with proteins like ERK and AKT important for transmitting extracellular signals into appropriate cellular responses. These pathways are key regulators of many physiological processes including cell cycle progression and apoptosis prevention.

Associated diseases and disorders

TROP2 is strongly associated with several types of cancers particularly epithelial cancers like breast and pancreatic cancer. Overexpression of TROP2 in tumors often correlates with poor prognosis and aggressive disease phenotypes. Interaction between TROP2 and proteins like EGFR in cancer cell signaling pathways amplifies tumor progression and metastasis. Understanding these associations makes TROP2 a significant target in developing therapeutic strategies for cancer treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Western blot - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Western blot - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Blocking/Diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

    All lanes: Western blot - Anti-TROP2 antibody [SP293] - C-terminal (Anti-TROP2 antibody [SP293] - C-terminal ab227689) at 1/100 dilution

    Lane 1: bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg

    Lane 2: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: LLC1 (mouse lung carcinoma) whole cell lysate at 20 µg

    Lane 4: B16F10 (Mouse skin melanoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 36 kDa

    Observed band size: 37 kDa

    Exposure time: 10s

    Blocking/Diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling TROP2 with Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/50 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 minutes. The section was incubated with Anti-TROP2 antibody [SP293] - C-terminal ab227689 for 10 mins at room temperature. The Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used. Membranous staining on the mouse skin. Performed on a Leica Biosystems BOND® RX instrument. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Flow Cytometry (Intracellular) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Intracellular Flow Cytometry analysis of SK-BR-3 (human mammary gland adenocarcinoma cell line) cells, labeling TROP2 with Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human esophagus tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human bladder tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human skin tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for TROP2 using Anti-TROP2 antibody [SP293] - C-terminal ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TROP2 antibody [SP293] - C-terminal ab227689).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com