AB227689

Anti-TROP2 antibody [SP293] - C-terminal

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(6 Publications)

Anti-TROP2 antibody [SP293] - C-terminal (ab227689) is a rabbit monoclonal antibody detecting TROP2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

GA733-1, M1S1, TROP2, TACSTD2, Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1

15 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human skin tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human tonsil tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human ovarian adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human lung squamous cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human prostate tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human skin squamous cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human bladder transitional cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human esophagus tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Flow Cytometry analysis of SK-BR-3 (human mammary gland adenocarcinoma cell line) cells, labeling TROP2 with ab227689 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human bladder tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling TROP2 with ab227689 at 1/50 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 minutes. The section was incubated with ab227689 for 10 mins at room temperature. The Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. Membranous staining on the mouse skin. Performed on a Leica Biosystems BOND® RX instrument. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Lab

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Blocking/Diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (ab227689) at 1/100 dilution

Lane 1:

bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg

Lane 2:

4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

LLC1 (mouse lung carcinoma) whole cell lysate at 20 µg

Lane 4:

B16F10 (Mouse skin melanoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 36 kDa

Observed band size: 37 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

All lanes:

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (ab227689) at 1/100 dilution

All lanes:

SK-BR-3 (human mammary gland adenocarcinoma cell line) cell lysate

Predicted band size: 36 kDa

false

Carrier free

Anti-TROP2 antibody [SP293] - BSA and Azide free
578 PE

PE Anti-TROP2 antibody [SP293] (C-terminal)
HRP

HRP Anti-TROP2 antibody [SP293] (C-terminal)
Alkaline Phosphatase

Alkaline Phosphatase Anti-TROP2 antibody [SP293] - C-terminal
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TROP2 antibody [SP293] - C-terminal
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TROP2 antibody [SP293] - C-terminal
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-TROP2 antibody [SP293] - C-terminal
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-TROP2 antibody [SP293] - C-terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-TROP2 antibody [SP293] - C-terminal
660 APC

APC Anti-TROP2 antibody [SP293] (C-terminal)
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-TROP2 antibody [SP293] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP293

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Boil tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature." }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Boil tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature." } } }

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Product details

What is this antibody validated in?
Anti-TROP2 antibody [SP293] - C-terminal (ab227689) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of TROP2?
Anti-TROP2 [SP293] - C-terminal (ab227689) specifically detects a band for TROP2 (UniProt: P09758) at a molecular weight of 36kDa.

Collaboration
Anti-TROP2 [SP293] - C-terminal (ab227689) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Other related products
We have a range of other formats of antibody clone [SP293] also available for your convenience: ab227689, Carrier free - ab272014, PE - ab306220, APC - ab306221, HRP - ab306222, Alkaline Phosphatase - ab309002, Alexa Fluor® 488 - ab309944, Alexa Fluor® 647 - ab310311, Alexa Fluor® 594 - ab310775, Alexa Fluor® 555 - ab312301, Alexa Fluor® 568 - ab312797, Alexa Fluor® 750 - ab321453

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TROP2 also known as TACSTD2 or tumor-associated calcium signal transducer 2 is a single-pass type I membrane glycoprotein. This protein is about 35 kDa in mass and primarily expresses in epithelial tissues. It acts as a transmembrane receptor that plays a role in signal transduction due to its domain structure binding and activation processes on the cell surface. TROP2 exhibits high expression in several types of tumors making it a potential target for therapeutic antibodies like anti-TROP2 anti trop2 or anti-TROP-2.

Biological function summary

TROP2 mediates calcium signaling and cell proliferation. It functions by being a part of a signaling complex on the cell membrane which affects multiple cellular activities such as cell growth motility and survival. Its role in maintaining the integrity of the epithelial cell layer also shows TROP2's significance in normal tissue homeostasis. The interaction of TROP2 with the extracellular matrix and other cell surface proteins support the structural integrity and communication between cells.

Pathways

TROP2 plays a central role in the PI3K/AKT and MAPK signaling pathways that regulate cell growth and survival. Within these pathways TROP2 works in concert with proteins like ERK and AKT important for transmitting extracellular signals into appropriate cellular responses. These pathways are key regulators of many physiological processes including cell cycle progression and apoptosis prevention.

TROP2 is strongly associated with several types of cancers particularly epithelial cancers like breast and pancreatic cancer. Overexpression of TROP2 in tumors often correlates with poor prognosis and aggressive disease phenotypes. Interaction between TROP2 and proteins like EGFR in cancer cell signaling pathways amplifies tumor progression and metastasis. Understanding these associations makes TROP2 a significant target in developing therapeutic strategies for cancer treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May function as a growth factor receptor.

See full target information TACSTD2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:30313 PubMed40830367

2025

Three-dimensional analysis of intraepidermal nerve fibres and Langerhans cells in keloids with a focus on pruritus.

Applications

Unspecified application

Species

Unspecified reactive species

Haruka Matsuzoe,Koh-Ei Toyoshima,Miki Takase,Ayako Tsuchiya,Mika Ikeda,Motoko Naitoh,Kazuya Kataoka,Tomoya Kawabata,Miho Ogawa,Naoki Morimoto,Takashi Tsuji

Cancer medicine 14:e70615 PubMed40059728

2025

Prognostic Value of Trop-2 Expression in Nonmetastatic Triple-Negative Breast Cancer and Correlation With Emerging Biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

William Jacot,Marie-Christine Chateau,Simon Thezenas,Séverine Guiu,Nelly Firmin,Virginie Lafont,Gwendal Lazennec,Charles Theillet,Florence Boissière-Michot

Current genomics 24:129-131 PubMed38178982

2024

Trop-2 as an Actionable Biomarker in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Cursano,Emanuele Frigo,Elham Sajjadi,Mariia Ivanova,Konstantinos Venetis,Elena Guerini-Rocco,Carmen Criscitiello,Giuseppe Curigliano,Nicola Fusco

Scientific reports 12:22498 PubMed36577919

2022

Correlation of TROP-2 expression with clinical-pathological characteristics and outcome in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hava Izci,Kevin Punie,Lise Waumans,Annouschka Laenen,Hans Wildiers,Freija Verdoodt,Christine Desmedt,Jan Ardui,Ann Smeets,Sileny N Han,Ines Nevelsteen,Patrick Neven,Giuseppe Floris

Scientific reports 12:9583 PubMed35688908

2022

TACSTD2 upregulation is an early reaction to lung infection.

Applications

Unspecified application

Species

Unspecified reactive species

Sára Lenárt,Peter Lenárt,Lucia Knopfová,Hana Kotasová,Vendula Pelková,Veronika Sedláková,Ondřej Vacek,Jana Pokludová,Vladimír Čan,Jan Šmarda,Karel Souček,Aleš Hampl,Petr Beneš

Oncology reports 40:759-766 PubMed29901160

2018

Trop2 is a potential biomarker for the promotion of EMT in human breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhao,Xingwang Kuai,Xueyi Zhou,Lizhou Jia,Jinsong Wang,Xiaobing Yang,Zhidan Tian,Xiaolei Wang,Qian Lv,Bin Wang,Youcai Zhao,Wenbin Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TROP2 antibody [SP293] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Flow Cytometry (Intracellular) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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