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AB238789

Anti-TROP2 antibody [SP294] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TROP2 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GA733-1, M1S1, TROP2, TACSTD2, Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1

16 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human skin tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human lung tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling TROP2 with ab227690 at 1/100 dilution (2.47 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human bladder carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227690 for 30 mins at room temperature. This image was generated using ab227690, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human cervix tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human breast ductal cell carcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TROP2 with ab227690 at 1/100 dilution (2.47 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on epithelium of the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227690 for 30 mins at room temperature. This image was generated using ab227690, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human prostate tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human bladder tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human breast tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP294] - BSA and Azide free (AB238789)

Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227690).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP294

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Boil tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature.</p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab238789 is the carrier-free version of ab227690.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TROP2 also known as TACSTD2 or tumor-associated calcium signal transducer 2 is a single-pass type I membrane glycoprotein. This protein is about 35 kDa in mass and primarily expresses in epithelial tissues. It acts as a transmembrane receptor that plays a role in signal transduction due to its domain structure binding and activation processes on the cell surface. TROP2 exhibits high expression in several types of tumors making it a potential target for therapeutic antibodies like anti-TROP2 anti trop2 or anti-TROP-2.
Biological function summary

TROP2 mediates calcium signaling and cell proliferation. It functions by being a part of a signaling complex on the cell membrane which affects multiple cellular activities such as cell growth motility and survival. Its role in maintaining the integrity of the epithelial cell layer also shows TROP2's significance in normal tissue homeostasis. The interaction of TROP2 with the extracellular matrix and other cell surface proteins support the structural integrity and communication between cells.

Pathways

TROP2 plays a central role in the PI3K/AKT and MAPK signaling pathways that regulate cell growth and survival. Within these pathways TROP2 works in concert with proteins like ERK and AKT important for transmitting extracellular signals into appropriate cellular responses. These pathways are key regulators of many physiological processes including cell cycle progression and apoptosis prevention.

TROP2 is strongly associated with several types of cancers particularly epithelial cancers like breast and pancreatic cancer. Overexpression of TROP2 in tumors often correlates with poor prognosis and aggressive disease phenotypes. Interaction between TROP2 and proteins like EGFR in cancer cell signaling pathways amplifies tumor progression and metastasis. Understanding these associations makes TROP2 a significant target in developing therapeutic strategies for cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as a growth factor receptor.
See full target information TACSTD2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1162669 PubMed37207208

2023

Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Charles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo Vilar
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com