Anti-Trophoblast specific protein alpha antibody [EPR28905-557] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trophoblast specific protein alpha antibody [EPR28905-557] - BSA and Azide free (AB320824)

This data was developed using ab320823, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Trophoblast specific protein alpha with ab320823 at 1/1000 (0.516 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse spleen.
The section was incubated with ab320823 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trophoblast specific protein alpha antibody [EPR28905-557] - BSA and Azide free (AB320824)

This data was developed using ab320823, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Trophoblast specific protein alpha with ab320823 at 1/1000 (0.516 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse liver (PMID : 16251741).
The section was incubated with ab320823 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Trophoblast specific protein alpha antibody [EPR28905-557] - BSA and Azide free (AB320824)

This data was developed using ab320823, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue staining LOX 1 with ab317689 at a 1/2000 dilution, ab324045 anti-AP2 gamma used at 1/500 dilution and ab320823 anti-Trophoblast specific protein alpha used at a 1/1000 dilution.

Panel A : merged staining of anti-LOX 1 (green; Opal™520), anti-AP2 gamma (magenta; Opal™690) and anti-Trophoblast specific protein alpha (gray; Opal™570) on mouse placenta.

Panel B : anti-LOX 1 staining trophoblast in mouse placenta.

Panel C : ant-AP2 gamma staining trophoblast stem cell in mouse placenta.

Panel D : ant-Trophoblast specific protein alpha staining junctional zone in mouse placenta.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317689, ab324045 and ab320823 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trophoblast specific protein alpha antibody [EPR28905-557] - BSA and Azide free (AB320824)

This data was developed using ab320823, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling Trophoblast specific protein alpha with ab320823 at 1/1000 (0.516 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in junctional zone of mouse placenta (PMID : 33884443).
The section was incubated with ab320823 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Dot

Supplier Data

Dot Blot - Anti-Trophoblast specific protein alpha antibody [EPR28905-557] - BSA and Azide free (AB320824)

This data was developed using ab320823, the same antibody clone in a different buffer formulation.

Dot blot analysis of Trophoblast specific protein alpha using ab320823 at 1 : 1000 (0.516 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Trophoblast specific protein alpha antibody [EPR28905-557] (<a href='/en-us/products/primary-antibodies/trophoblast-specific-protein-alpha-antibody-epr28905-557-ab320823'>ab320823</a>) at 1/1000 dilution

All lanes:

His-tagged mouse Trophoblast specific protein alpha recombinant protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s