AB229679

Anti-Tropomodulin 1 antibody - C-terminal

Name: Anti-Tropomodulin 1 antibody - C-terminal (ab229679) | Abcam
Brand: Abcam Limited
SKU: AB229679
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Tropomodulin 1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TMOD1.

View Alternative Names

D9S57E, TMOD, TMOD1, Tropomodulin-1, Erythrocyte tropomodulin, E-Tmod

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Tropomodulin 1 antibody - C-terminal (AB229679)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tropomodulin 1 antibody - C-terminal (AB229679)

4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Tropomodulin 1 (green) using ab229679 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Supplier Data

Western blot - Anti-Tropomodulin 1 antibody - C-terminal (AB229679)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-Tropomodulin 1 antibody - C-terminal (ab229679) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg

Lane 3:

IMR32 whole cell extract at 30 µg

Lane 4:

SK-N-AS whole cell extract at 30 µg

Predicted band size: 41 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human TMOD1. The exact immunogen used to generate this antibody is proprietary information.

P28289

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tropomodulin 1 also known as TMOD1 is a capping protein responsible for regulating actin filament dynamics by binding to the pointed end of actin filaments preventing filament elongation and depolymerization. This interaction involves actin-binding sites and tropomyosin-binding regions providing stability to the filament structure. Tropomodulin 1 has an approximate mass of 40 kDa and is expressed in multiple tissues predominantly found in skeletal and cardiac muscles where it plays an essential role in maintaining the integrity of muscle fibers.

Biological function summary

Tropomodulin 1 controls the length of actin filaments impacting muscle contraction and cell motility. It forms part of a larger complex with tropomyosin assisting in stabilizing thin filaments. By effectively managing actin filament architecture Tropomodulin 1 contributes to structural cellular functions supporting cell morphology and intracellular transport. Its interaction with tropomyosin facilitates the proper functioning of contractile systems in muscle cells.

Pathways

Tropomodulin 1 participates in the sarcomere organization and muscle contraction pathways where it ensures the appropriate arrangement of the actin cytoskeleton. Tropomodulin 1’s regulation of actin-tropomyosin interactions affects proteins such as troponin which plays an important role in the regulation of calcium binding during muscle contractions. These pathways are critical for muscle development and proper neuromuscular functionality.

Mutations or dysregulation of Tropomodulin 1 have been implicated in cardiomyopathies and nemaline myopathy. These conditions often result from mismanagement of actin filament lengths affecting muscle fiber stability. Tropomodulin 1’s pathological role often involves disruptions in its binding with tropomyosin and such imbalances can lead to severe muscular disorders. By affecting the actin cytoskeleton Tropomodulin 1 connections to proteins such as actin and tropomyosin become significant in understanding these muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Blocks the elongation and depolymerization of the actin filaments at the pointed end (PubMed : 38168645). The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton. May play an important role in regulating the organization of actin filaments by preferentially binding to a specific tropomyosin isoform at its N-terminus.

See full target information TMOD1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Canadian journal of physiology and pharmacology 100:422-431 PubMed34860610

2021

β-elemene relieves neuropathic pain in mice through the regulation on C-X-C motif chemokine receptor 3 and GABAA receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Dai,Zhenhua Zeng,Shuo Deng,Sanbao Zou,Tingyang Dou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tropomodulin 1 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-Tropomodulin 1 antibody - C-terminal (AB229679)

Western blot - Anti-Tropomodulin 1 antibody - C-terminal (AB229679)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise