Rabbit Polyclonal Tropomodulin 1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TMOD1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TMOD1. The exact immunogen used to generate this antibody is proprietary information. Database link P28289
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Blocks the elongation and depolymerization of the actin filaments at the pointed end (PubMed:38168645). The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton. May play an important role in regulating the organization of actin filaments by preferentially binding to a specific tropomyosin isoform at its N-terminus.
Alternative names
D9S57E, TMOD, TMOD1, Tropomodulin-1, Erythrocyte tropomodulin, E-Tmod
Recommended products
Rabbit Polyclonal Tropomodulin 1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TMOD1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TMOD1. The exact immunogen used to generate this antibody is proprietary information. Database link P28289
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tropomodulin 1 also known as TMOD1 is a capping protein responsible for regulating actin filament dynamics by binding to the pointed end of actin filaments preventing filament elongation and depolymerization. This interaction involves actin-binding sites and tropomyosin-binding regions providing stability to the filament structure. Tropomodulin 1 has an approximate mass of 40 kDa and is expressed in multiple tissues predominantly found in skeletal and cardiac muscles where it plays an essential role in maintaining the integrity of muscle fibers.
- Biological function summary
Tropomodulin 1 controls the length of actin filaments impacting muscle contraction and cell motility. It forms part of a larger complex with tropomyosin assisting in stabilizing thin filaments. By effectively managing actin filament architecture Tropomodulin 1 contributes to structural cellular functions supporting cell morphology and intracellular transport. Its interaction with tropomyosin facilitates the proper functioning of contractile systems in muscle cells.
- Pathways
Tropomodulin 1 participates in the sarcomere organization and muscle contraction pathways where it ensures the appropriate arrangement of the actin cytoskeleton. Tropomodulin 1’s regulation of actin-tropomyosin interactions affects proteins such as troponin which plays an important role in the regulation of calcium binding during muscle contractions. These pathways are critical for muscle development and proper neuromuscular functionality.
- Associated diseases and disorders
Mutations or dysregulation of Tropomodulin 1 have been implicated in cardiomyopathies and nemaline myopathy. These conditions often result from mismanagement of actin filament lengths affecting muscle fiber stability. Tropomodulin 1’s pathological role often involves disruptions in its binding with tropomyosin and such imbalances can lead to severe muscular disorders. By affecting the actin cytoskeleton Tropomodulin 1 connections to proteins such as actin and tropomyosin become significant in understanding these muscle-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Tropomodulin 1 antibody - C-terminal (ab229679)
12% SDS-PAGE gel.
All lanes: Western blot - Anti-Tropomodulin 1 antibody - C-terminal (ab229679) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg
Lane 2: SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg
Lane 3: IMR32 whole cell extract at 30 µg
Lane 4: SK-N-AS whole cell extract at 30 µg
Predicted band size: 41 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Tropomodulin 1 antibody - C-terminal (ab229679)
4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Tropomodulin 1 (green) using ab229679 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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