AB124833

Anti-Tropomodulin 2 antibody [EPR6353]

Name: Anti-Tropomodulin 2 antibody [EPR6353] (ab124833) | Abcam
Brand: Abcam Limited
SKU: AB124833
Price: 300 USD
Availability: InStock
Rating: 3 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(2 Publications)

Rabbit Recombinant Monoclonal Tropomodulin 2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NTMOD, TMOD2, Tropomodulin-2, Neuronal tropomodulin, N-Tmod

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 2 antibody [EPR6353] (AB124833)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 2 antibody [EPR6353] (AB124833)

ab124833 at 1/250 dilution staining Tropomodulin 2 in paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Tropomodulin 2 antibody [EPR6353] (AB124833)

All lanes:

Western blot - Anti-Tropomodulin 2 antibody [EPR6353] (ab124833) at 1/10000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

OI-RD Scanning - Anti-Tropomodulin 2 antibody [EPR6353] (AB124833)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Tropomodulin 2 antibody [EPR6353] (AB124833)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Tropomodulin 2 antibody [EPR6353] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6353

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tropomodulin 2 also known as Tmod2 is a protein that regulates the actin cytoskeleton dynamics by capping the pointed end of actin filaments. It prevents the addition and loss of actin subunits at this end stabilizing the filament structure. With an approximate molecular weight of 40 kDa Tropomodulin 2 is expressed mainly in the brain but levels vary in different neural tissues. This protein shares functional similarities with Tropomodulin isoforms particularly in its role of filament capping.

Biological function summary

The Tropomodulin 2 protein plays an important role in maintaining neural tissue integrity by influencing actin filament stability which affects cellular structures and signaling. Its association with other Tropomodulin proteins such as Tmod1 and Tmod4 suggests a multi-protein complex role in actin filament control. This actin stabilization is vital for synaptic structure and neuronal growth which are critical processes for effective neurocommunication.

Pathways

Tropomodulin 2 is actively involved in cytoskeletal dynamics that are important for pathways like those in neurodevelopment and synaptic function. Within these pathways it interacts with proteins such as tropomyosin which also binds to actin and enhances filament stabilization. Tropomodulin 2's function in these pathways impacts cellular morphology and motility impacting overall neurological development and maintenance.

Abnormal expression or dysfunction of Tropomodulin 2 has been linked to neurological disorders including schizophrenia and neurodegenerative diseases. Variations in expression affect not only actin filament dynamics but also impact proteins like β-III spectrin which associates with the cytoskeleton abnormalities seen in these disorders. This connection highlights the importance of Tropomodulin 2 in maintaining normal neural function and how disruptions can lead to disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton (By similarity).

See full target information TMOD2

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Marine drugs 18: PubMed31963558

2020

Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihua Sun,Manqi Zhangsun,Shuai Dong,Yiqiao Liu,Jiang Qian,Dongting Zhangsun,Sulan Luo

OncoTargets and therapy 12:8961-8976 PubMed31802906

2019

Key Genes And Pathways Controlled By E2F1 In Human Castration-Resistant Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qingniao Zhou,Chengbang Wang,Yuanyuan Zhu,Qunying Wu,Yonghua Jiang,Yuanjie Huang,Yanling Hu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tropomodulin 2 antibody [EPR6353]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 2 antibody [EPR6353] (AB124833)

Western blot - Anti-Tropomodulin 2 antibody [EPR6353] (AB124833)

OI-RD Scanning - Anti-Tropomodulin 2 antibody [EPR6353] (AB124833)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise