Rabbit Recombinant Monoclonal Tropomodulin 2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | Flow Cyt | WB | |
---|---|---|---|
Human | Tested | Not recommended | Tested |
Mouse | Predicted | Not recommended | Predicted |
Rat | Predicted | Not recommended | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton (By similarity).
NTMOD, TMOD2, NTMOD, Tropomodulin-2, Neuronal tropomodulin, N-Tmod
Rabbit Recombinant Monoclonal Tropomodulin 2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR6353
Affinity purification Protein A
1.28 x 10-10 M
Blue Ice
+4°C
+4°C
Do Not Freeze
ab248026 is the carrier-free version of Anti-Tropomodulin 2 antibody [EPR6353] ab124833.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Tropomodulin 2 also known as Tmod2 is a protein that regulates the actin cytoskeleton dynamics by capping the pointed end of actin filaments. It prevents the addition and loss of actin subunits at this end stabilizing the filament structure. With an approximate molecular weight of 40 kDa Tropomodulin 2 is expressed mainly in the brain but levels vary in different neural tissues. This protein shares functional similarities with Tropomodulin isoforms particularly in its role of filament capping.
The Tropomodulin 2 protein plays an important role in maintaining neural tissue integrity by influencing actin filament stability which affects cellular structures and signaling. Its association with other Tropomodulin proteins such as Tmod1 and Tmod4 suggests a multi-protein complex role in actin filament control. This actin stabilization is vital for synaptic structure and neuronal growth which are critical processes for effective neurocommunication.
Tropomodulin 2 is actively involved in cytoskeletal dynamics that are important for pathways like those in neurodevelopment and synaptic function. Within these pathways it interacts with proteins such as tropomyosin which also binds to actin and enhances filament stabilization. Tropomodulin 2's function in these pathways impacts cellular morphology and motility impacting overall neurological development and maintenance.
Abnormal expression or dysfunction of Tropomodulin 2 has been linked to neurological disorders including schizophrenia and neurodegenerative diseases. Variations in expression affect not only actin filament dynamics but also impact proteins like β-III spectrin which associates with the cytoskeleton abnormalities seen in these disorders. This connection highlights the importance of Tropomodulin 2 in maintaining normal neural function and how disruptions can lead to disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-Tropomodulin 2 antibody [EPR6353] ab124833, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Tropomodulin 2 antibody [EPR6353] (Anti-Tropomodulin 2 antibody [EPR6353] ab124833) at 1/10000 dilution
Lane 1: Fetal brain tissue lysate at 10 µg
Lane 2: SH-SY5Y cell lysate at 10 µg
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
This data was developed using Anti-Tropomodulin 2 antibody [EPR6353] ab124833, the same antibody clone in a different buffer formulation.Anti-Tropomodulin 2 antibody [EPR6353] ab124833, at 1/250 dilution, staining Tropomodulin 2 in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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