Anti-Tropomyosin 1 (alpha) antibody [EPR5158] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] - BSA and Azide free (AB247885)

This data was developed using ab109505, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1 : 600 (0.343 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] - BSA and Azide free (AB247885)

This data was developed using ab109505, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1 : 600 (0.343 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] - BSA and Azide free (AB247885)

This data was developed using ab109505, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1 : 600 (0.343 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] - BSA and Azide free (AB247885)

This data was developed using ab109505, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1 : 600 (0.343 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• WB

Supplier Data

Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] - BSA and Azide free (AB247885)

This data was developed using ab109505, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (<a href='/en-us/products/primary-antibodies/tropomyosin-1-alpha-antibody-epr5158-ab109505'>ab109505</a>) at 1/1000 dilution

Lane 1:

Human heart lysate at 15 µg

Lane 2:

Mouse heart lysate at 20 µg

Lane 3:

Rat heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

• WB

Supplier Data

Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] - BSA and Azide free (AB247885)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This data was developed using ab109505, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (<a href='/en-us/products/primary-antibodies/tropomyosin-1-alpha-antibody-epr5158-ab109505'>ab109505</a>) at 1/1000 dilution

Lane 1:

MYC and DDK-tagged Recombinant Human TPM1 protein (Full length)

Lane 2:

MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)

Lane 3:

MYC and DDK-tagged Recombinant Human TPM3 protein (Full length)

Lane 4:

GST-tagged Recombinant Human TPM4 protein (Full length)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 37 kDa

false

Exposure time: 60s