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AB133292

Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159]

5

(3 Reviews)

|

(8 Publications)

Rabbit Recombinant Monoclonal TPM1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

C15orf13, TMSA, TPM1, Tropomyosin alpha-1 chain, Alpha-tropomyosin, Tropomyosin-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (AB133292)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (AB133292)

Imunohistochemical analysis of paraffin-embedded Human muscle tissue labelling Tropomyosin 1 (alpha) with ab133292 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (AB133292)
  • WB

Supplier Data

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (AB133292)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds. The band sizes observed are consistent with PMID : 9168473, PMID : 22965424, PMID : 16765662.

All lanes:

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (ab133292) at 1/1000 dilution

Lane 1:

HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 3:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 4:

NIN/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 7:

Human skeletal muscle tissue lysate, at 20 µg

Lane 8:

Mouse skeletal muscle tissue lysate, at 20 µg

Lane 9:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 37 kDa,32 kDa

false

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (AB133292)
  • WB

Supplier Data

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (AB133292)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 20 seconds.

All lanes:

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (ab133292) at 1/1000 dilution

Lane 1:

MYC and DDK-tagged Recombinant Human TPM1 protein (Full length)

Lane 2:

MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)

Lane 3:

MYC and DDK-tagged Recombinant Human TPM3 protein (Full length)

Lane 4:

GST-tagged Recombinant Human TPM4 protein (Full length)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5159

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tropomyosin 1 (alpha) and Tropomyosin 3 are actin-binding proteins with key roles in stabilizing actin filaments within muscle and non-muscle cells. Often referred to as TPM1 and TPM3 these proteins have molecular masses of approximately 32 kDa and 33 kDa respectively. TPM1 and TPM3 are expressed in various tissues such as heart skeletal muscle and brain. They help maintain structural integrity of the cytoskeleton by interacting with actin filaments providing essential support for cellular functions.
Biological function summary

Tropomyosin isoforms bind along the length of actin filaments regulating their interaction with myosin and other actin-binding proteins. TPM1 and TPM3 form part of a complex that includes troponin contributing to the calcium-dependent regulation of muscle contraction. In non-muscle cells they regulate cell shape cytokinesis and intracellular transport. Through their role in stabilizing actin filaments these proteins influence many cellular processes.

Pathways

TPM1 and TPM3 are essential for muscle contraction and cytoskeletal dynamics. They play significant roles in the actin cytoskeleton and cardiac muscle contraction pathways. Tropomyosins interact with proteins like myosin and troponin to modulate muscle contraction. They ensure effective actin-myosin interaction by allowing proper binding and release cycles during contraction and relaxation.

TPM1 and TPM3 mutations have associations with conditions such as hypertrophic cardiomyopathy and nemaline myopathy. These genetic mutations can alter their ability to regulate actin filament function contributing to abnormal contraction and muscle weakness. In hypertrophic cardiomyopathy TPM1 mutations often occur alongside mutations in proteins like myosin heavy chain and cardiac myosin-binding protein C compounding the disease's effects and presenting critical challenges in cardiac function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to actin filaments in muscle and non-muscle cells (PubMed : 23170982). Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction (PubMed : 23170982). Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
See full target information TPM1

Additional targets

TPM3
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:1352 PubMed38409164

2024

Targeting HDAC6 to treat heart failure with preserved ejection fraction in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Ranjbarvaziri,Aliya Zeng,Iris Wu,Amara Greer-Short,Farshad Farshidfar,Ana Budan,Emma Xu,Reva Shenwai,Matthew Kozubov,Cindy Li,Melissa Van Pell,Francis Grafton,Charles E MacKay,Xiaomei Song,James R Priest,Gretchen Argast,Mohammad A Mandegar,Timothy Hoey,Jin Yang

Journal of molecular and cellular cardiology plus 6:100050 PubMed39802622

2023

Western diet triggers cardiac dysfunction in heterozygous -targeted knock-in mice: A two-hit model of hypertrophic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Edgar E Nollet,Sila Algül,Max Goebel,Saskia Schlossarek,Nicole N van der Wel,Judith J M Jans,Mark A van de Wiel,Jaco C Knol,Thang V Pham,Sander R Piersma,Richard de Goeij-de Haas,Jill Hermans,Jan Bert van Klinken,Michel van Weeghel,Riekelt H Houtkooper,Lucie Carrier,Connie R Jimenez,Diederik W D Kuster,Jolanda van der Velden

Nature food 3:74-85 PubMed37118488

2022

A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Messmer,Iva Klevernic,Carolina Furquim,Ekaterina Ovchinnikova,Arin Dogan,Helder Cruz,Mark J Post,Joshua E Flack

Pharmaceutical biology 58:1244-1251 PubMed33332205

2020

Arecoline suppresses epithelial cell viability by upregulating tropomyosin-1 through the transforming growth factor-β/Smad pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Long Li,Liqun Gu,Zhigang Yao,Yuehong Wang,Zhangui Tang,Xiaoying Wu

Respiratory research 20:53 PubMed30857524

2019

Long non-coding RNA CASC2 suppresses pulmonary artery smooth muscle cell proliferation and phenotypic switch in hypoxia-induced pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Junsong Gong,Zujun Chen,Yu Chen,Huanran Lv,Haisong Lu,Fuxia Yan,Lihuan Li,Weili Zhang,Jia Shi

Proceedings of the National Academy of Sciences of 114:11956-11961 PubMed29078393

2017

HSPB7 is indispensable for heart development by modulating actin filament assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Tongbin Wu,Yongxin Mu,Julius Bogomolovas,Xi Fang,Jennifer Veevers,Roberta B Nowak,Christopher T Pappas,Carol C Gregorio,Sylvia M Evans,Velia M Fowler,Ju Chen

Scientific reports 7:12343 PubMed28955040

2017

Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model.

Applications

WB

Species

Pig

Aleksandra Binek,Rodrigo Fernández-Jiménez,Inmaculada Jorge,Emilio Camafeita,Juan Antonio López,Navratan Bagwan,Carlos Galán-Arriola,Andres Pun,Jaume Agüero,Valentin Fuster,Borja Ibanez,Jesús Vázquez

Cell biochemistry and biophysics 67:127-38 PubMed23695782

2013

Diggin' on u(biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates.

Applications

Unspecified application

Species

Unspecified reactive species

Carrie E Rubel,Jonathan C Schisler,Eric D Hamlett,Robert M DeKroon,Mathias Gautel,Oscar Alzate,Cam Patterson
View all publications
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Product promise

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