AB240056

Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TPM1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 1 publication.

View Alternative Names

C15orf13, TMSA, TPM1, Tropomyosin alpha-1 chain, Alpha-tropomyosin, Tropomyosin-1, Tropomyosin alpha-3 chain, Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5, hTM5, TPM3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] - BSA and Azide free (AB240056)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] - BSA and Azide free (AB240056)

Imunohistochemical analysis of paraffin-embedded Human muscle tissue labelling Tropomyosin 1 (alpha) with ab133292 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133292).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] - BSA and Azide free (AB240056)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133292). Blocking and Diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds. The band sizes observed are consistent with PMID : 9168473, PMID : 22965424, PMID : 16765662.

All lanes:

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (<a href='/en-us/products/primary-antibodies/tropomyosin-1-alpha-tropomyosin-3-antibody-epr5159-ab133292'>ab133292</a>) at 1/1000 dilution

Lane 1:

HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 3:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 4:

NIN/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 7:

Human skeletal muscle tissue lysate, at 20 µg

Lane 8:

Mouse skeletal muscle tissue lysate, at 20 µg

Lane 9:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 37 kDa,32 kDa

false

Supplier Data

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] - BSA and Azide free (AB240056)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 20 seconds.

All lanes:

Lane 1:

MYC and DDK-tagged Recombinant Human TPM1 protein (Full length)

Lane 2:

MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)

Lane 3:

MYC and DDK-tagged Recombinant Human TPM3 protein (Full length)

Lane 4:

GST-tagged Recombinant Human TPM4 protein (Full length)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Unconjugated

Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5159

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab240056 is the carrier-free version of ab133292.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tropomyosin 1 (alpha) and Tropomyosin 3 are actin-binding proteins with key roles in stabilizing actin filaments within muscle and non-muscle cells. Often referred to as TPM1 and TPM3 these proteins have molecular masses of approximately 32 kDa and 33 kDa respectively. TPM1 and TPM3 are expressed in various tissues such as heart skeletal muscle and brain. They help maintain structural integrity of the cytoskeleton by interacting with actin filaments providing essential support for cellular functions.

Biological function summary

Tropomyosin isoforms bind along the length of actin filaments regulating their interaction with myosin and other actin-binding proteins. TPM1 and TPM3 form part of a complex that includes troponin contributing to the calcium-dependent regulation of muscle contraction. In non-muscle cells they regulate cell shape cytokinesis and intracellular transport. Through their role in stabilizing actin filaments these proteins influence many cellular processes.

Pathways

TPM1 and TPM3 are essential for muscle contraction and cytoskeletal dynamics. They play significant roles in the actin cytoskeleton and cardiac muscle contraction pathways. Tropomyosins interact with proteins like myosin and troponin to modulate muscle contraction. They ensure effective actin-myosin interaction by allowing proper binding and release cycles during contraction and relaxation.

TPM1 and TPM3 mutations have associations with conditions such as hypertrophic cardiomyopathy and nemaline myopathy. These genetic mutations can alter their ability to regulate actin filament function contributing to abnormal contraction and muscle weakness. In hypertrophic cardiomyopathy TPM1 mutations often occur alongside mutations in proteins like myosin heavy chain and cardiac myosin-binding protein C compounding the disease's effects and presenting critical challenges in cardiac function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to actin filaments in muscle and non-muscle cells (PubMed : 23170982). Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction (PubMed : 23170982). Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

See full target information TPM1

Additional targets

TPM3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 9:1055356 PubMed36518851

2022

The copper transporter CTR1 and cisplatin accumulation at the single-cell level by LA-ICP-TOFMS.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Schoeberl,Michael Gutmann,Sarah Theiner,Mario Corte-Rodríguez,Gabriel Braun,Petra Vician,Walter Berger,Gunda Koellensperger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] - BSA and Azide free (AB240056)

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] - BSA and Azide free (AB240056)

Western blot - Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] - BSA and Azide free (AB240056)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise