AB250178

Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Tropomyosin 2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

TMSB, TPM2, Tropomyosin beta chain, Beta-tropomyosin, Tropomyosin-2

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Supplier Data

Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (AB250178)

This data was developed using ab180176, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Human fetal muscle lysate labeling Tropomyosin 2 using ab180176 at 1/10.

All lanes:

Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (<a href='/en-us/products/primary-antibodies/tropomyosin-2-antibody-epr12846-c-terminal-ab180176'>ab180176</a>)

Predicted band size: 33 kDa

true

Supplier Data

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (AB250178)

This data was developed using ab180176, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (<a href='/en-us/products/primary-antibodies/tropomyosin-2-antibody-epr12846-c-terminal-ab180176'>ab180176</a>) at 1/1000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HT-1080 cell lysate at 10 µg

Lane 4:

Fetal muscle lysate at 10 µg

Secondary

All lanes:

Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

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Supplier Data

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (AB250178)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

This data was developed using ab180176, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (<a href='/en-us/products/primary-antibodies/tropomyosin-2-antibody-epr12846-c-terminal-ab180176'>ab180176</a>) at 1/1000 dilution

Lane 1:

MYC and DDK-tagged Recombinant Human TPM1 protein (Full length)

Lane 2:

MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)

Lane 3:

MYC and DDK-tagged Recombinant Human TPM3 protein (Full length)

Lane 4:

GST-tagged Recombinant Human TPM4 protein (Full length)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 42 kDa

false

Exposure time: 180s

Unconjugated

Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12846

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab250178 is the carrier-free version of ab180176.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tropomyosin 2 (TPM2) is a protein that plays an important mechanical role in muscle function. It is a component of the actin filament system weighing approximately 32.7 kDa. TPM2 interacts with actin to stabilize the filament structures which are essential in muscle contraction processes. This protein is expressed mainly in muscle tissues including skeletal and cardiac muscles but is also present in other tissues. Its stability and interaction with actin filaments make it significant in maintaining sarcomere structure.

Biological function summary

TPM2 ensures proper muscle contraction by regulating the interaction between actin and myosin two key components in muscle movement. It forms a complex with actin and troponin a critical protein that regulates muscle contraction through calcium ion binding. This complex facilitates muscle fiber contraction by exposing the binding sites for myosin on actin filaments. The presence of TPM2 in the complex is essential for the contraction mechanism allowing precise control of muscle movements.

Pathways

TPM2 acts in the regulation of muscle contraction and actin cytoskeleton signaling pathways. In the muscle contraction pathway TPM2 interacts closely with tropomyosin 1 (TPM1) and the myosin motor protein playing a regulatory role. It is involved in the actin cytoskeleton signaling pathway where it helps organize actin filaments necessary for various cellular activities. Through these pathways TPM2 integrates signals essential for muscle activity and cellular architecture maintenance.

TPM2 mutations have a notable connection to conditions such as nemaline myopathy and distal arthrogryposis. Nemaline myopathy is characterized by muscle weakness and it arises due to defects in sarcomere proteins with TPM2 being a significant player. Additionally changes in TPM2 can associate with contractures and joint deformities in distal arthrogryposis. The protein connects to cellular disorders through its interactions with troponin I (TNNI2) and other structural sarcomere components highlighting its critical role in muscle integrity and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.

See full target information TPM2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free

Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (AB250178)

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (AB250178)

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (AB250178)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise