AB224355

Anti-Tropomyosin 3 antibody

Name: Anti-Tropomyosin 3 antibody (ab224355) | Abcam
Brand: Abcam Limited
SKU: AB224355
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Tropomyosin 3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TPM3 aa 1-200.

View Alternative Names

Tropomyosin alpha-3 chain, Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5, hTM5, TPM3

3 Images

Supplier Data

Western blot - Anti-Tropomyosin 3 antibody (AB224355)

All lanes:

Western blot - Anti-Tropomyosin 3 antibody (ab224355) at 1/100 dilution

All lanes:

THP-1 (human monocytic leukemia cell line) cell lysate

Predicted band size: 33 kDa

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Supplier Data

Western blot - Anti-Tropomyosin 3 antibody (AB224355)

All lanes:

Western blot - Anti-Tropomyosin 3 antibody (ab224355) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 33 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 3 antibody (AB224355)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 3 antibody (AB224355)

Paraffin-embedded human skeletal muscle tissue stained for Tropomyosin 3 using ab224355 at 1/50 in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TPM3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P06753

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tropomyosin 3 also known as TPM3 is an actin-binding protein that stabilizes actin filaments. This protein plays an important role in maintaining cytoskeleton integrity and regulating cell contraction. Tropomyosin 3 has a molecular weight of approximately 32.5 kDa and is widely expressed in various tissues especially in muscle cells and in some non-muscle tissues. This protein interacts with other proteins to form complexes that modulate the dynamics of the actin filament systems.

Biological function summary

Tropomyosin 3 modulates the access of other actin-binding proteins to the actin filament by covering and uncovering binding sites. It partners with actin and troponin complexes influencing muscle contraction and cell motility. Additionally TPM3 regulates the stability and flexibility of actin cortex important for cell shape and movement. In non-muscle cells it supports the formation of cell structures needed for signaling and transportation.

Pathways

Tropomyosin 3 functions in the regulation of the actin cytoskeleton pathway and the muscle contraction pathway. It coordinates with proteins like actin and myosin to facilitate these processes. In the actin cytoskeleton pathway TPM3 balances polymerization and depolymerization of actin important for maintaining cellular structures. The muscle contraction pathway relies on TPM3 to regulate calcium binding supporting muscular function and responsiveness.

Tropomyosin 3 mutations link to conditions like congenital myopathy and nemaline myopathy. Mutations in TPM3 affect muscle fiber regulation leading to muscle weakness and structural abnormalities. Congenital myopathy involves other proteins such as nebulin which cooperate with TPM3 in maintaining muscle architecture. In nemaline myopathy improper expression or structure of TPM3 contributes to defective sarcomere formation impairing muscle function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

See full target information TPM3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 12: PubMed33535184

2021

Evaluation of the prognostic values of solute carrier (SLC) family 39 genes for patients with lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhou,Yaoqi Zhu,Huizhong Qi,Liang Liang,Hao Wu,Jingping Yuan,Qingyong Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tropomyosin 3 antibody

Western blot - Anti-Tropomyosin 3 antibody (AB224355)

Western blot - Anti-Tropomyosin 3 antibody (AB224355)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 3 antibody (AB224355)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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