Mouse Monoclonal Troponin I fast skeletal muscle antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Human, Recombinant fragment samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNNI2.
IgG1
Mouse
Preservative: 0.05% Sodium azide
Constituents: PBS
Liquid
Monoclonal
Flow Cyt | ELISA | WB | ICC/IF | |
---|---|---|---|---|
Human | Expected | Expected | Tested | Tested |
Mouse | Tested | Expected | Expected | Expected |
Recombinant fragment | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/200 - 1/400 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment | Dilution info 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Select an associated product type
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
TNNI2
Mouse Monoclonal Troponin I fast skeletal muscle antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Human, Recombinant fragment samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNNI2.
IgG1
Mouse
Preservative: 0.05% Sodium azide
Constituents: PBS
Liquid
Monoclonal
2F12G2
Affinity purification Protein G
Purified from tissue culture supernatant.
Blue Ice
1-2 weeks
+4°C
-20°C
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This supplementary information is collated from multiple sources and compiled automatically.
Troponin I fast skeletal muscle also known as TNNI2 is a protein involved in muscle contraction. It weighs around 21.2 kDa and is mainly expressed in fast-twitch skeletal muscle. TNNI2 acts by inhibiting actomyosin ATPase activity meaning it helps prevent muscle contraction until it receives a signal to contract. It binds to actin and plays a regulatory role in the muscle contraction process.
Troponin I fast skeletal muscle interacts within the troponin complex which includes Troponin T and Troponin C. Located on the thin filament of the muscle fiber this complex modulates the contraction of striated muscle by controlling calcium binding. When calcium binds to Troponin C it induces a conformational change that displaces TNNI2 from actin allowing for muscle contraction to occur.
Troponin I fast skeletal muscle has essential functions in the calcium modulation pathways specific to muscle contraction. It plays an important role in the regulation of muscle contraction by interacting with calcium ions and other proteins such as tropomyosin. As part of the excitation-contraction coupling pathway TNNI2 helps ensure the proper contraction of muscles in response to neural stimulation. Additionally its role in these pathways involves specific interactions with proteins like Troponin C and Troponin T which are necessary for functional muscle contraction.
Abnormalities in Troponin I fast skeletal muscle can lead to muscle-related conditions such as nemaline myopathy and distal arthrogryposis. These disorders affect muscle structure and function leading to muscle weakness or deformities. Mutations in TNNI2 may result in improper interaction with other sarcomeric proteins such as tropomyosin disrupting normal muscle contraction and leading to the mentioned diseases. Understanding the role of TNNI2 in these disorders can aid in the development of diagnostic and therapeutic strategies.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Flow cytometric analysis of NIH/3T3 cells using ab126824 AT 1/200 (green) and negative control (red).
All lanes: Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution
Lane 1: HEK293 cell lysate
Lane 2: TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 21 kDa
Example of standard curves obtained using ab126824. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red)
Immunofluorescence analysis of Hela cells using ab126824
at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
All lanes: Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution
All lanes: Recombinant Human TNNI2 protein (AA: 1-182)
Predicted band size: 21 kDa
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