AB126824

Anti-Troponin I fast skeletal muscle antibody [2F12G2]

Name: Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) | Abcam
Brand: Abcam Limited
SKU: AB126824

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(2 Publications)

Mouse Monoclonal Troponin I fast skeletal muscle antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Human, Recombinant fragment samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNNI2.

View Alternative Names

TNNI2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

Immunofluorescence analysis of Hela cells using ab126824 at 1/200 (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

Flow Cyt

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Flow Cytometry - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

Flow cytometric analysis of NIH/3T3 cells using ab126824 AT 1/200 (green) and negative control (red).

ELISA - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

ELISA

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ELISA - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

Example of standard curves obtained using ab126824. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red)

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Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

All lanes:

Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 21 kDa

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Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

All lanes:

Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution

All lanes:

Recombinant Human TNNI2 protein (AA: 1-182)

Predicted band size: 21 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2F12G2

Isotype

IgG1

Carrier free

Reacts with

Human, Mouse

Applications

ICC/IF, WB, Flow Cyt, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TNNI2.

P48788

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/10000", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Troponin I fast skeletal muscle also known as TNNI2 is a protein involved in muscle contraction. It weighs around 21.2 kDa and is mainly expressed in fast-twitch skeletal muscle. TNNI2 acts by inhibiting actomyosin ATPase activity meaning it helps prevent muscle contraction until it receives a signal to contract. It binds to actin and plays a regulatory role in the muscle contraction process.

Biological function summary

Troponin I fast skeletal muscle interacts within the troponin complex which includes Troponin T and Troponin C. Located on the thin filament of the muscle fiber this complex modulates the contraction of striated muscle by controlling calcium binding. When calcium binds to Troponin C it induces a conformational change that displaces TNNI2 from actin allowing for muscle contraction to occur.

Pathways

Troponin I fast skeletal muscle has essential functions in the calcium modulation pathways specific to muscle contraction. It plays an important role in the regulation of muscle contraction by interacting with calcium ions and other proteins such as tropomyosin. As part of the excitation-contraction coupling pathway TNNI2 helps ensure the proper contraction of muscles in response to neural stimulation. Additionally its role in these pathways involves specific interactions with proteins like Troponin C and Troponin T which are necessary for functional muscle contraction.

Abnormalities in Troponin I fast skeletal muscle can lead to muscle-related conditions such as nemaline myopathy and distal arthrogryposis. These disorders affect muscle structure and function leading to muscle weakness or deformities. Mutations in TNNI2 may result in improper interaction with other sarcomeric proteins such as tropomyosin disrupting normal muscle contraction and leading to the mentioned diseases. Understanding the role of TNNI2 in these disorders can aid in the development of diagnostic and therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNI2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Reproductive sciences (Thousand Oaks, Calif.) 27:100-109 PubMed32046398

2020

Trisomy 21 is Associated with Caspase-2 Upregulation in Cytotrophoblasts at the Maternal-Fetal Interface.

Applications

Unspecified application

Species

Unspecified reactive species

Daisy Leon-Martinez,Joshua F Robinson,Tamara Zdravkovic,Olga Genbacev,Matthew Gormley,Michael Mcmaster,Susan J Fisher,Katherine Bianco

Journal of tissue engineering and regenerative medicine 12:e918-e936 PubMed28101909

2017

Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Selwa Mokhtar Boularaoui,Khaled M A Abdel-Raouf,Noaf Salah Ali Alwahab,Megan E Kondash,George A Truskey,Jeremy Choon Meng Teo,Nicolas Christoforou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Troponin I fast skeletal muscle antibody [2F12G2]

Immunocytochemistry/ Immunofluorescence - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

Flow Cytometry - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

ELISA - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise