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Mouse Monoclonal Troponin I fast skeletal muscle antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Human, Recombinant fragment samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNNI2.

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Images

Flow Cytometry - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824), expandable thumbnail
  • Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824), expandable thumbnail
  • ELISA - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824), expandable thumbnail
  • Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (AB126824), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human TNNI2. Database link P48788

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytELISAWBICC/IF
Human
Expected
Expected
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Recombinant fragment
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/200 - 1/400

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment

Dilution info

1/10000

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500 - 1/2000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200 - 1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Alternative names

Recommended products

Mouse Monoclonal Troponin I fast skeletal muscle antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Human, Recombinant fragment samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNNI2.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human TNNI2. Database link P48788
Clone number

2F12G2

Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Troponin I fast skeletal muscle also known as TNNI2 is a protein involved in muscle contraction. It weighs around 21.2 kDa and is mainly expressed in fast-twitch skeletal muscle. TNNI2 acts by inhibiting actomyosin ATPase activity meaning it helps prevent muscle contraction until it receives a signal to contract. It binds to actin and plays a regulatory role in the muscle contraction process.

Biological function summary

Troponin I fast skeletal muscle interacts within the troponin complex which includes Troponin T and Troponin C. Located on the thin filament of the muscle fiber this complex modulates the contraction of striated muscle by controlling calcium binding. When calcium binds to Troponin C it induces a conformational change that displaces TNNI2 from actin allowing for muscle contraction to occur.

Pathways

Troponin I fast skeletal muscle has essential functions in the calcium modulation pathways specific to muscle contraction. It plays an important role in the regulation of muscle contraction by interacting with calcium ions and other proteins such as tropomyosin. As part of the excitation-contraction coupling pathway TNNI2 helps ensure the proper contraction of muscles in response to neural stimulation. Additionally its role in these pathways involves specific interactions with proteins like Troponin C and Troponin T which are necessary for functional muscle contraction.

Associated diseases and disorders

Abnormalities in Troponin I fast skeletal muscle can lead to muscle-related conditions such as nemaline myopathy and distal arthrogryposis. These disorders affect muscle structure and function leading to muscle weakness or deformities. Mutations in TNNI2 may result in improper interaction with other sarcomeric proteins such as tropomyosin disrupting normal muscle contraction and leading to the mentioned diseases. Understanding the role of TNNI2 in these disorders can aid in the development of diagnostic and therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824), expandable thumbnail

    Flow Cytometry - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)

    Flow cytometric analysis of NIH/3T3 cells using ab126824 AT 1/200 (green) and negative control (red).

  • Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824), expandable thumbnail

    Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)

    All lanes: Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution

    Lane 1: HEK293 cell lysate

    Lane 2: TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 21 kDa

  • ELISA - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824), expandable thumbnail

    ELISA - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)

    Example of standard curves obtained using ab126824. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red)

  • Immunocytochemistry/ Immunofluorescence - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)

    Immunofluorescence analysis of Hela cells using ab126824
    at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824), expandable thumbnail

    Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)

    All lanes: Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution

    All lanes: Recombinant Human TNNI2 protein (AA: 1-182)

    Predicted band size: 21 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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