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AB134838

Anti-Troponin I fast skeletal muscle antibody

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(3 Publications)

Rabbit Polyclonal Troponin I fast skeletal muscle antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TNNI2.

View Alternative Names

TNNI2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin I fast skeletal muscle antibody (AB134838)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin I fast skeletal muscle antibody (AB134838)

Immunohistochemistry analysis of formalin fixed, paraffin embedded Human Skeletal Muscle tissue labelling Troponin I fast skeletal muscle with ab134838 at 5 µg/ml.

Western blot - Anti-Troponin I fast skeletal muscle antibody (AB134838)
  • WB

Unknown

Western blot - Anti-Troponin I fast skeletal muscle antibody (AB134838)

All lanes:

Western blot - Anti-Troponin I fast skeletal muscle antibody (ab134838) at 1/200 dilution

All lanes:

Human Brain lysate

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human TNNI2. The exact immunogen used to generate this antibody is proprietary information.

P48788

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Troponin I fast skeletal muscle also known as TNNI2 is a protein involved in muscle contraction. It weighs around 21.2 kDa and is mainly expressed in fast-twitch skeletal muscle. TNNI2 acts by inhibiting actomyosin ATPase activity meaning it helps prevent muscle contraction until it receives a signal to contract. It binds to actin and plays a regulatory role in the muscle contraction process.
Biological function summary

Troponin I fast skeletal muscle interacts within the troponin complex which includes Troponin T and Troponin C. Located on the thin filament of the muscle fiber this complex modulates the contraction of striated muscle by controlling calcium binding. When calcium binds to Troponin C it induces a conformational change that displaces TNNI2 from actin allowing for muscle contraction to occur.

Pathways

Troponin I fast skeletal muscle has essential functions in the calcium modulation pathways specific to muscle contraction. It plays an important role in the regulation of muscle contraction by interacting with calcium ions and other proteins such as tropomyosin. As part of the excitation-contraction coupling pathway TNNI2 helps ensure the proper contraction of muscles in response to neural stimulation. Additionally its role in these pathways involves specific interactions with proteins like Troponin C and Troponin T which are necessary for functional muscle contraction.

Abnormalities in Troponin I fast skeletal muscle can lead to muscle-related conditions such as nemaline myopathy and distal arthrogryposis. These disorders affect muscle structure and function leading to muscle weakness or deformities. Mutations in TNNI2 may result in improper interaction with other sarcomeric proteins such as tropomyosin disrupting normal muscle contraction and leading to the mentioned diseases. Understanding the role of TNNI2 in these disorders can aid in the development of diagnostic and therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNI2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Forensic science international 301:284-288 PubMed31195249

2019

The Troponin-I fast skeletal muscle is reliable marker for the determination of vitality in the suicide hanging.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra De Matteis,Massimiliano dell'Aquila,Aniello Maiese,Paola Frati,Raffaele La Russa,Giorgio Bolino,Vittorio Fineschi

Journal of oleo science 68:141-148 PubMed30713267

2019

Investigation of the Lipid Changes That Occur in Hypertrophic Muscle due to Fish Protein-feeding Using Mass Spectrometry Imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Mizuki Morisasa,Naoko Goto-Inoue,Tomohiko Sato,Kazumasa Machida,Mina Fujitani,Taro Kishida,Kenji Uchida,Tsukasa Mori

Human molecular genetics 26:2526-2540 PubMed28453658

2017

Androgen receptor agonists increase lean mass, improve cardiopulmonary functions and extend survival in preclinical models of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Suriyan Ponnusamy,Ryan D Sullivan,Dahui You,Nadeem Zafar,Chuan He Yang,Thirumagal Thiyagarajan,Daniel L Johnson,Maron L Barrett,Nikki J Koehler,Mayra Star,Erin J Stephenson,Dave Bridges,Stephania A Cormier,Lawrence M Pfeffer,Ramesh Narayanan
View all publications
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Product promise

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