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Rabbit Recombinant Monoclonal Troponin I fast skeletal muscle antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-Troponin I fast skeletal muscle antibody [EPR14926] - BSA and Azide free (AB190175), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Alternative names

TNNI2

Recommended products

Rabbit Recombinant Monoclonal Troponin I fast skeletal muscle antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14926
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab190175 is the carrier-free version of Anti-Troponin I fast skeletal muscle antibody [EPR14926] ab183508.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Troponin I fast skeletal muscle also known as TNNI2 is a protein involved in muscle contraction. It weighs around 21.2 kDa and is mainly expressed in fast-twitch skeletal muscle. TNNI2 acts by inhibiting actomyosin ATPase activity meaning it helps prevent muscle contraction until it receives a signal to contract. It binds to actin and plays a regulatory role in the muscle contraction process.

Biological function summary

Troponin I fast skeletal muscle interacts within the troponin complex which includes Troponin T and Troponin C. Located on the thin filament of the muscle fiber this complex modulates the contraction of striated muscle by controlling calcium binding. When calcium binds to Troponin C it induces a conformational change that displaces TNNI2 from actin allowing for muscle contraction to occur.

Pathways

Troponin I fast skeletal muscle has essential functions in the calcium modulation pathways specific to muscle contraction. It plays an important role in the regulation of muscle contraction by interacting with calcium ions and other proteins such as tropomyosin. As part of the excitation-contraction coupling pathway TNNI2 helps ensure the proper contraction of muscles in response to neural stimulation. Additionally its role in these pathways involves specific interactions with proteins like Troponin C and Troponin T which are necessary for functional muscle contraction.

Associated diseases and disorders

Abnormalities in Troponin I fast skeletal muscle can lead to muscle-related conditions such as nemaline myopathy and distal arthrogryposis. These disorders affect muscle structure and function leading to muscle weakness or deformities. Mutations in TNNI2 may result in improper interaction with other sarcomeric proteins such as tropomyosin disrupting normal muscle contraction and leading to the mentioned diseases. Understanding the role of TNNI2 in these disorders can aid in the development of diagnostic and therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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