Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)]

• IP

Supplier Data

Immunoprecipitation - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (AB184554)

Western blot analysis of Troponin I fast skeletal muscle in Human skeletal muscle lysate immunoprecipitated using ab184554 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of Human skeletal muscle lysate.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554)

Predicted band size: 21 kDa

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• WB

Supplier Data

Western blot - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (AB184554)

All lanes:

Western blot - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilution

All lanes:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

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• WB

Supplier Data

Western blot - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (AB184554)

All lanes:

Western blot - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilution

Lane 1:

Mouse muscle lysate at 10 µg

Lane 2:

Rat muscle lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

• WB

CiteAb

Western blot - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (AB184554)

Troponin I fast skeletal muscle western blot using anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] ab184554. Publication image and figure legend from Wang, S., Zuo, H., et al., 2019, Cell Death Dis, PubMed 31243262.

ab184554 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab184554 please see the product overview.

Knockdown of Neat1 improves muscle growth in vivo.a The injection scheme for LV3-shNeat1 or LV3-shNC particles into the right or left hindlimb muscles of C57 mice. The injection was given every one week for one month. b qPCR results showing that Neat1 expression was reduced after LV3-shNeat1 particle injection, while the mRNA expression of Myog, Myhc, α-actin, and Tnni2 was significantly increased after LV3-shNeat1 particle injection. c Western blot analysis showing that the protein expression of Myog, Myhc, α-actin, and Tnni2 was increased after LV3-shNeat1 particle injection. The protein levels of these genes were quantified using ImageJ software. d Representative photograph of the three muscles from the right or left hindlimbs showing that the volume of the Qu, TA, and Gas muscles of the right hindlimb were larger than those of the left hindlimb. e Quantification of the weight of three muscles from the right or left hindlimbs of 12 injected mice showing that the weight of the Qu, TA, and Gas muscles of the right hindlimb were higher than those of the left hindlimb. f Representative photograph of myosin immunofluorescence staining in Qu, TA, and Gas muscles from the right or left hindlimb following injection with LV3-shNeat1 or LV3-shNC particles. Compared with LV3-shNC particle injection, LV3-shNeat1 particle injection increased the average cross-sectional areas of the indicated muscles. The fiber sizes of the Qu, TA, and Gas muscles were quantified using ImageJ. Relative RNA and protein levels were normalized to those of Gapdh. All values represent the mean ± s.d. of three independent experiments. *p < 0.05, **p < 0.01

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