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AB224583

Anti-Troponin T1/TNT antibody

5

(1 Review)

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(1 Publication)

Rabbit Polyclonal Troponin T1/TNT antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TNNT1 aa 1-100.

View Alternative Names

TNT, TNNT1, TnTs, Slow skeletal muscle troponin T, sTnT

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin T1/TNT antibody (AB224583)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin T1/TNT antibody (AB224583)

Negative Control : Paraffin-embedded human kidney tissue stained for Troponin T1/TNT using ab224583 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin T1/TNT antibody (AB224583)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin T1/TNT antibody (AB224583)

Negative Control : Paraffin-embedded human lymph node tissue stained for Troponin T1/TNT using ab224583 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin T1/TNT antibody (AB224583)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin T1/TNT antibody (AB224583)

Paraffin-embedded human skeletal muscle tissue stained for Troponin T1/TNT using ab224583 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin T1/TNT antibody (AB224583)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin T1/TNT antibody (AB224583)

Negative Control : Paraffin-embedded human colon tissue stained for Troponin T1/TNT using ab224583 at 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TNNT1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P13805

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Troponin T1 also known as TNT or skeletal muscle Troponin T is an important player in muscle contraction. This protein interacts with actin and tropomyosin to regulate the calcium-mediated contraction process. Troponin T1 has a molecular mass of approximately 36 kDa. It is strongly expressed in skeletal and cardiac muscles. This protein provides critical structural roles linking the components of the troponin complex together which in turn influences muscle contraction efficiency.
Biological function summary

This protein is a component of the troponin complex which also includes the proteins Troponin I and Troponin C. Troponin T1 binds tropomyosin and helps to align the troponin complex along the actin filament. This alignment is needed for the regulation of actin-myosin crossbridge formation. Through these interactions Troponin T1 allows skeletal and cardiac muscles to respond to calcium concentration changes during contraction.

Pathways

Troponin T1 participates mainly in the calcium signaling pathway and plays an important role in muscle contraction pathways. Its interaction with Troponin C affects calcium binding dynamics which is important for muscle contraction cycles. This coordination with Troponin I helps inhibit ATPase activity based on calcium presence ensuring efficient muscle relaxation following contraction.

Mutations in the Troponin T1 gene are linked to skeletal muscle diseases like nemaline myopathy. This condition affects muscle tone and strength mostly impacting infants and children. Troponin mutations also connect to hypertrophic cardiomyopathy a disorder that thickens the heart muscle potentially leading to heart failure. Mutations in genes encoding these proteins can disrupt normal muscle contraction cycles leading to these serious health issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 41:111571 PubMed36323262

2022

Nuclear stabilization of p53 requires a functional nucleolar surveillance pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine M Hannan,Priscilla Soo,Mei S Wong,Justine K Lee,Nadine Hein,Perlita Poh,Kira D Wysoke,Tobias D Williams,Christian Montellese,Lorey K Smith,Sheren J Al-Obaidi,Lorena Núñez-Villacís,Megan Pavy,Jin-Shu He,Kate M Parsons,Karagh E Loring,Tess Morrison,Jeannine Diesch,Gaetan Burgio,Rita Ferreira,Zhi-Ping Feng,Cathryn M Gould,Piyush B Madhamshettiwar,Johan Flygare,Thomas J Gonda,Kaylene J Simpson,Ulrike Kutay,Richard B Pearson,Christoph Engel,Nicholas J Watkins,Ross D Hannan,Amee J George
View all publications
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Product promise

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