Rabbit Polyclonal TROVE2/SS-A antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RO60 aa 1 to C-terminus.
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Publications
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- The EMBO journal :e1049482020Receptor-mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmelia E Ohnstad et. al.PubMed 33226137
- Life sciences 242:1172072019TROVE2 strengthens the anti-inflammatory effect via macrophage polarization by estrogen induction in abdominal aortic aneurysm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanshan Chen,Yuanyuan Zhao,Huang Jin,Xiaoyu Qi,Jinrong He,Juan Huang,Yu Ding,Weiqun Chen,Chuangyan Wu,Xiangchao Ding,Shanshan Chen,Hao Zhang,Yuqiang Shang,Dunfeng Du,Jiajia ZhaoPubMed 31863777
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RO60 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P10155
Reactivity data
Select an application| ELISA | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Expected | Predicted | Predicted | Predicted |
| Rat | Expected | Tested | Expected | Expected |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs (PubMed:18056422, PubMed:26382853). Binds to endogenous Alu retroelements which are induced by type I interferon and stimulate porinflammatory cytokine secretion (PubMed:26382853). Regulates the expression of Alu retroelements as well as inflammatory genes (PubMed:26382853). May play roles in cilia formation and/or maintenance (By similarity).
Alternative names
SSA2, TROVE2, RO60, RNA-binding protein RO60, 60 kDa SS-A/Ro ribonucleoprotein, Ro 60 kDa autoantigen, Sjoegren syndrome antigen A2, Sjoegren syndrome type A antigen, TROVE domain family member 2, 60 kDa Ro protein, 60 kDa ribonucleoprotein Ro, RoRNP, Ro60 autoantigen, SS-A
Recommended products
Rabbit Polyclonal TROVE2/SS-A antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RO60 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RO60 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P10155
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
>95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TROVE2 also known as SS-A or Ro60 is a ribonucleoprotein with molecular mass approximately 60 kDa. The protein is present mainly in the cytoplasm and nucleus of eukaryotic cells. TROVE2 functions as an essential part of the Ro ribonucleoprotein complex which binds non-coding RNA to protect it from degradation. This protein plays a role in recognizing misfolded RNA molecules and assists in their correct folding or degradation ensuring RNA quality control in the cell.
- Biological function summary
TROVE2 is involved in several important cellular processes. The protein often exists as part of a complex together with La protein and small Y RNAs contributing to RNA biogenesis and stability. It helps regulate the cellular stress response by acting on misfolded RNAs and interacts with various RNA molecules through specific binding mechanisms. TROVE2's role in maintaining RNA integrity suggests it has a wider impact on the stability of the transcriptome.
- Pathways
The protein TROVE2 integrates into immune response and RNA quality control pathways. It associates with molecules involved in RNA surveillance like the La protein and modulates the cell's reaction to environmental stress and pathogenic challenges. TROVE2 influences the pathways connected to RNA processing and degradation therefore intersecting with networks ensuring proper cellular function and stress management.
- Associated diseases and disorders
TROVE2 shows a connection with autoimmune disorders such as Sjögren's syndrome and systemic lupus erythematosus (SLE). These diseases often feature the production of autoantibodies against TROVE2 associating it with immune dysregulation. The interplay between TROVE2 and the La protein is notable in these conditions as both participate in producing autoantibodies contributing to the pathology of these autoimmune syndromes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Western blot - Anti-TROVE2/SS-A antibody (ab194004)
All lanes: Western blot - Anti-TROVE2/SS-A antibody (ab194004) at 3 µg/mL
Lane 1: MCF-7 whole cell lysate
Lane 2: HeLa whole cell lysate
Lane 3: PC3 whole cell lysate
Lane 4: Mouse heart tissue
Lane 5: Mouse spleen tissue
Lane 6: Mouse lung tissue
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/5000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TROVE2/SS-A antibody (ab194004)
Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling TROVE2/SS-A with ab194004 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROVE2/SS-A antibody (ab194004)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling TROVE2/SS-A with ab194004 at dilution of 1/100.
Western blot - Anti-TROVE2/SS-A antibody (ab194004)
All lanes: Western blot - Anti-TROVE2/SS-A antibody (ab194004) at 2 µg/mL
Lane 1: HeLa whole cell lysate
Lane 2: 293T whole cell lysate
SecondaryAll lanes: Polyclonal goat anti-rabbit at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TROVE2/SS-A antibody (ab194004)
Immunocytochemistry/Immunofluorescence analysis of PC3 cells labelling TROVE2/SS-A with ab194004 at a dilution of 1/100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROVE2/SS-A antibody (ab194004)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling TROVE2/SS-A with ab194004 at 1/20 dilution.
Western blot - Anti-TROVE2/SS-A antibody (ab194004)
All lanes: Western blot - Anti-TROVE2/SS-A antibody (ab194004) at 3.4 µg/mL
Lane 1: HeLa whole cell lysate
Lane 2: Rat kidney tissue lysate
SecondaryAll lanes: Polyclonal goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROVE2/SS-A antibody (ab194004)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TROVE2/SS-A with ab194004 at 1/20 dilution.
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