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AB251474

Anti-TROVE2/SS-A antibody [EPR20322] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TROVE2/SS-A antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

View Alternative Names

SSA2, TROVE2, RO60, RNA-binding protein RO60, 60 kDa SS-A/Ro ribonucleoprotein, Ro 60 kDa autoantigen, Sjoegren syndrome antigen A2, Sjoegren syndrome type A antigen, TROVE domain family member 2, 60 kDa Ro protein, 60 kDa ribonucleoprotein Ro, RoRNP, Ro60 autoantigen, SS-A

4 Images
Western blot - Anti-TROVE2/SS-A antibody [EPR20322] - BSA and Azide free (AB251474)
  • WB

Supplier Data

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] - BSA and Azide free (AB251474)

This data was developed using ab207416, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (<a href='/en-us/products/primary-antibodies/trove2-ss-a-antibody-epr20322-ab207416'>ab207416</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

Exposure time: 2s

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] - BSA and Azide free (AB251474)
  • WB

Supplier Data

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] - BSA and Azide free (AB251474)

This data was developed using ab207416, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-7 : 30 seconds; Lane 8 : 3 minutes.

All lanes:

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (<a href='/en-us/products/primary-antibodies/trove2-ss-a-antibody-epr20322-ab207416'>ab207416</a>) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Lane 3:

Rat kidney lysate at 10 µg

Lane 4:

Rat spleen lysate at 10 µg

Lane 5:

Mouse brain lysate at 10 µg

Lane 6:

Mouse kidney lysate at 10 µg

Lane 7:

Mouse spleen lysate at 10 µg

Lane 8:

Mouse heart lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] - BSA and Azide free (AB251474)
  • WB

Supplier Data

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] - BSA and Azide free (AB251474)

This data was developed using ab207416, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (<a href='/en-us/products/primary-antibodies/trove2-ss-a-antibody-epr20322-ab207416'>ab207416</a>) at 1/10000 dilution

Lane 1:

MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 3:

293T (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

Exposure time: 15s

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] - BSA and Azide free (AB251474)
  • WB

Supplier Data

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] - BSA and Azide free (AB251474)

This data was developed using ab207416, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (<a href='/en-us/products/primary-antibodies/trove2-ss-a-antibody-epr20322-ab207416'>ab207416</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

Exposure time: 2s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20322

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251474 is the carrier-free version of ab207416.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TROVE2 also known as SS-A or Ro60 is a ribonucleoprotein with molecular mass approximately 60 kDa. The protein is present mainly in the cytoplasm and nucleus of eukaryotic cells. TROVE2 functions as an essential part of the Ro ribonucleoprotein complex which binds non-coding RNA to protect it from degradation. This protein plays a role in recognizing misfolded RNA molecules and assists in their correct folding or degradation ensuring RNA quality control in the cell.
Biological function summary

TROVE2 is involved in several important cellular processes. The protein often exists as part of a complex together with La protein and small Y RNAs contributing to RNA biogenesis and stability. It helps regulate the cellular stress response by acting on misfolded RNAs and interacts with various RNA molecules through specific binding mechanisms. TROVE2's role in maintaining RNA integrity suggests it has a wider impact on the stability of the transcriptome.

Pathways

The protein TROVE2 integrates into immune response and RNA quality control pathways. It associates with molecules involved in RNA surveillance like the La protein and modulates the cell's reaction to environmental stress and pathogenic challenges. TROVE2 influences the pathways connected to RNA processing and degradation therefore intersecting with networks ensuring proper cellular function and stress management.

TROVE2 shows a connection with autoimmune disorders such as Sjögren's syndrome and systemic lupus erythematosus (SLE). These diseases often feature the production of autoantibodies against TROVE2 associating it with immune dysregulation. The interplay between TROVE2 and the La protein is notable in these conditions as both participate in producing autoantibodies contributing to the pathology of these autoimmune syndromes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs (PubMed : 18056422, PubMed : 26382853). Binds to endogenous Alu retroelements which are induced by type I interferon and stimulate porinflammatory cytokine secretion (PubMed : 26382853). Regulates the expression of Alu retroelements as well as inflammatory genes (PubMed : 26382853). May play roles in cilia formation and/or maintenance (By similarity).
See full target information RO60
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1180 PubMed40775066

2025

Regulation of cortical neurogenesis by MED13L via transcriptional priming and its implications for MED13L syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Li,Yu-Zhu Hao,Jia Cui,Xiao-Xiao Cao,Nan Wu,Ji Ma,Shuang-Yi Xie,Yi-Hsuan Pan,Xue-Lian He,Yu-Lan Zhao,Xiao-Bing Yuan
View all publications
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Product promise

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